Biomedical subjects

C Wester

Publications and source records attributed to C Wester.

4 recordsLinked to original sources

PubMed2006

Gene-expression profiling of HIV-1 infection and perinatal transmission in Botswana.

Perinatal transmission of human immunodeficiency virus (HIV)-1 represents a major problem in many regions of the world, especially Southern Africa. With the exception of viral and proviral load, the role for maternal cofactors in perinatal transmission outcome is largely unknown. In this study, an assessment was made of peripheral blood mononuclear cells (PBMC) gene-expression profiles to better understand transcriptional changes associated with HIV-1 infection and perinatal transmission among young adult mothers with infants in Botswana. Peripheral blood mononuclear cells specimens were used from 25 HIV+ drug naive and 20 HIV- healthy mothers, similar in age and location, collected in 1999-2000 and 2003, and processed with the exact same methods, as previously described. Expression profiling of 22 277 microarray gene probes implicated a broad initiation of innate response gene-sets, including toll-like receptor, interferon-stimulated and antiviral RNA response pathways in association with maternal HIV-1 infection. Maternal transmission status was further associated with host genes that influence RNA processing and splicing patterns. In addition to real-time polymerase chain reaction validation of specific genes, enriched category validation of PBMC profiles was conducted using two independent data sets for either HIV-1 infection or an unrelated RNA virus, severe acute respiratory virus infection. HIV-1 pathogen-specific host profiles should prove a useful tool in infection and transmission intervention efforts worldwide.

Adolescent↗

PubMed2003

Validation of the clinical bulbocavernosus reflex.

AIMS: To validate the clinically obtained bulbocavernosus reflex (BCR) by comparing it to the results of clitoroanal reflex (CAR) electrodiagnostic testing. MATERIALS AND METHODS: Thirty three women with detrusor overactivity underwent clinical examination and electrodiagnostic testing including evaluation of CAR latency using a Nicolet Viking IIe electrodiagnostic instrument, stimulating the paraclitoral area on each side in turn with paired stimuli while recording anal sphincter compound muscle action potentials bilaterally using surface patch electrodes. The results of clinical and electrodiagnostic testing were compared. RESULTS: The BCR was clinically present on at least one side in 26 (79%) women, and thought to be clinically bilaterally absent in 7 (21%). Electrophysiologic testing revealed CARs to be at least unilaterally present in 30 (91%) patients. When the BCR was clinically present, a CAR was recorded in 92% of patients. Of the seven women with clinically absent BCR, just one had absent CARs. CONCLUSIONS: In this sample of women with detrusor overactivity, the clinical BCR did not have strong correlation with the electrically obtained reflex.

Action Potentials↗

PubMed1998

Normal pelvic floor physiology.

This article outlines the normal physiology of the female pelvic floor, including normal urinary storage and voiding, normal colorectal storage, and defecation. Physiologic changes during a woman's lifetime that may affect bladder and bowel function are also considered. An important framework for understanding the normal physiology of the female pelvic floor is provided, so the reader may gain a more thoughtful approach to the recognition and treatment of pelvic floor pathophysiology.

Colon↗

PubMed1997

Influence of visual distractors on detectability of liver nodules on contrast-enhanced spiral computed tomography scans.

RATIONALE AND OBJECTIVES: The authors evaluated the ability of observers to identify simulated nodules placed electronically on normal contrast material-enhanced computed tomography (CT) scans of the liver to assess the effect of nodule size and polarity on detection and localization. METHODS: Seven readers evaluated two sets of CT scans that contained 80 stimuli each. The simulated nodules were either darker or brighter than the contrast-enhanced liver and were 5.6-8.0 mm in diameter. Readers were asked to find the most suspicious-looking nodule on each section and rate the likelihood that the chosen location actually contained a nodule. RESULTS: The fraction of nodules found by each observer was substantially greater for dark nodules than for bright ones (0.679 +/- 0.03 vs 0.345 +/- 0.045, respectively [mean +/- standard error]). This difference was consistent for all nodule sizes. Additional analyses (including receiver operating characteristic curves of conditional responses) suggested that the presence of bright blood vessels distracted the readers and decreased their ability to find bright nodules. CONCLUSION: Normal vascular structures on contrast-enhanced CT scans of the liver impair an observer's ability to detect bright liver nodules.

Humans↗