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C Wiegand

Publications and source records attributed to C Wiegand.

34 records · Page 2Linked to original sources
PubMed1990

Characterization of the coated vesicle uncoating ATPase: tissue distribution, association with and activity on intact coated vesicles.

We have analyzed the uncoating process of clathrin-coated vesicles (CV) performed by an ATPase (UA; apparent molecular mass 70 kDa) prepared from various mammalian tissues. Our data show that this enzyme removes the clathrin coat from isolated, intact coated vesicles, as seen by sedimentation analysis on gels and also by electron microscopy. The isolated UA does not discriminate between CV from homologous or heterologous tissues. This finding implies that the brain-specific insertion in clathrin light chains cannot be essential for the binding of brain UA to target vesicles. Polyclonal antibodies were raised against UA and were found to inhibit UA activity. Immunoblotting of purified CV and immunoblotting of CV in situ indicate that a subpopulation of CV contains bound UA. However, most of the uncoating enzyme is not associated with coated structures in mammalian tissue culture cells. Our data support the hypothesis that the 70 kDa uncoating ATPase is responsible for the in vivo uncoating of coated vesicles.

Adenosine Triphosphatases↗
PubMed1987

[Possibility of errors in conventional Doppler sonography of the carotid arteries].

Results of Doppler sonography and angiography were compared in 100 consecutive patients with ischaemia in the area of carotid arterial supply. In 15 there were important differences. Three Doppler "occlusions" were angiographically demonstrated to be carotid arterial stenoses; significant additional information was provided by angiography in two patients with stenoses at the origin of the internal carotid artery, while in ten--despite unremarkable Doppler findings--angiography revealed disease in the carotids.

Adolescent↗
PubMed1987

Labeling of structural elements at the ventral plasma membrane of fibroblasts with the immunogold technique.

We investigated the organization of two structural elements (microfilaments and clathrin coated structures) at the ventral plasma membrane in fibroblasts. The methods employed included exposure of the inner face of the ventral plasma membrane by lysis-squirting, labeling the exposed structures with specific antibodies conjugated with colloidal gold, and platinum-carbon replica techniques. The results demonstrate that this regimen combines good ultrastructural preservation with unambiguous identification of structural elements associated with the plasma membrane. This method is therefore reliable and can be generally used to explore in detail the ultrastructural organization of the plasma membrane.

Actin Cytoskeleton↗
PubMed1986

[Knowledge of central pontine myelinolysis].

By a case study, the authors point out the significance of a too rapid correction of hyponatremia for the development of central pontine myelinolysis. The regional anatomy of the lesion is discussed, and reference is made to the differential diagnostic spectrum.

Adult↗
PubMedDate not supplied

[Cannabinoid intake by passive smoking].

The presence of cannabinoids in the urine can be produced by passive inhalation of hashish resp. marihuana smoke. The height depends on the intensity of exposure. Under extreme conditions concentrations between 40 to 50 ng/ml of cannabinoids had been found in the urine. In regard to the maximum variation-coefficient of the immunoassay methods, which is about 30%, we think it is useful to define a threshold value of 65 ng/ml of cannabinoids to distinguish between active and passive inhalation.

Adult↗