Biomedical subjects
Publications and source records attributed to C Y Liao.
The melting behavior of Ge nanocrystals embedded within SiO2 is evaluated using in situ transmission electron microscopy. The observed melting-point hysteresis is large (+/-17%) and nearly symmetric about the bulk melting point. This hysteresis is modeled successfully using classical nucleation theory without the need to invoke epitaxy.
BACKGROUND: The human skin, especially the sebaceous gland, is a steroidogenic organ similar to the gonads and adrenal cortex, possessing all the enzymes required for steroid sex-hormone synthesis and metabolism. Factors regulating cutaneous steroidogenesis associated with disease status remain largely unknown. OBJECTIVE: We hypothesized that transcription factors involved in sex formation and regulation of steroidogenesis in the classical steroidogenic organs are also expressed in the sebaceous glands. Their possible role in the pathogenesis of acne were investigated. METHODS: We used reverse transcription polymerase chain reaction (RT-PCR), in situ hybridization and Western blotting to analyse the expression of SF-1, WT-1, SRY, SOX-9 and DAX-1 mRNAs and their proteins in cultured human sebocytes and the facial skin of acne patients. RESULTS: The in situ hybridization study showed SOX-9 mRNA mainly localized in basal keratinocytes, the basal layer of the sebaceous glands and eccrine glands. Immortalized human sebaceous gland cells (SZ95) expressed mRNA for SOX-9, WT-1 and DAX-1 but not for SF-1 or SRY. The expression of DAX-1 protein was slightly inhibited by 10(-6) m oestradiol (E2) at 6 h but enhanced by 10(-6) m dihydrotestosterone (DHT) at 48 h. The facial expression of SOX-9 seemed to be higher in the acne-prone male patients, while DAX-1 was stronger in subjects without acne, although both were statistically insignificant. CONCLUSION: Our findings confirm the expression of some sex-determining genes in human sebaceous glands. Further studies on a larger patient population including the normal controls are needed to elucidate the functional significance of these transcription factors in the pathogenesis of acne.
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We present a description of the dynamics of a supercooled binary Lennard-Jones liquid in term of the potential landscape of the system. The slowing down of the dynamics in supercooled liquids near the kinetic glass transition is related to the existence of basins in the potential landscape. The inherent structures that are the local potential minima in the configuration space obtained by a quench process are employed to represent the configurations of the basins. We present time correlation functions of the inherent structure, both the self and the coherent part, as a function of wave vector. We also calculated the mean-square displacement, and the non-Gaussian parameter of the van Hove self-correlation function. Compared with the dynamics of original configurations, the short-time relaxation has almost been eliminated by the quench process. However, the long-time alpha relaxations remain essentially the same. We conclude that the long-time alpha relaxation is the result of cross-basin transition in the potential landscape in the configuration space.
Previously, we showed that vaccination with the glycoprotein D (gD) gene of pseudorabies virus (PrV) delivered by Escherichia coli induced protective immune responses. In this study, we report that oral DNA vaccination with attenuated Salmonella choleraesuis carrying the PrV gD gene conferred protective immunity in mice against PrV. Moreover, co-delivery of the prothymosin alpha gene carried by S. choleraesuis enhanced the vaccine efficacy. Our results thus demonstrate for the first time, to our knowledge, the effectiveness of oral DNA vaccination using S. choleraesuis as a delivery vehicle and the potential usefulness of prothymosin alpha as a DNA vaccine adjuvant.
The short wavelength density fluctuation of DLPC (dilaurylphosphatidylcholine) bilayers close to full hydration has been studied by the inelastic x-ray scattering technique below and above the main transition temperature. The analysis based on a generalized three effective eigenmode theory allows us to construct the dispersion relation of the high frequency sound mode for the first time. The marked softening of the excitation near k = 14 nm(-1), corresponding to the lipid chain-chain correlation peak in the structure factor, in the L(alpha) phase implies prevalent occurrences of short-wavelength in-plane motions of lipid chains that might be of importance for transportation of small molecules across membranes.
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A double haploid (DH) population and a recombinant inbred (RI) population derived from a cross between a japonica male parent Azucena and indica female parents, IR64 and IR1552 respectively, were used in both field and pot experiments for detecting QTLs and epistasis for rice panicle length in different genetic background and different environments. Panicle length (PL) was measured at maturity. QTLs for PL were detected using single marker analysis and interval mapping. Epistasis effects on the trait were also analyzed. Nine QTLs were detected in DH population, including 5 QTLs detected from field experiment and 4 from pot experiment, among them 3 QTLs mapped on chromosomes 1 and 4 were identified in both field and pot experiments. No significant epistasis effect was detected for PL. Four QTLs were detected in RI population, among them 2 from field condition and 2 from pot experiment. 6 pairs of epistasis loci were detected in RI population. One QTL mapped on chromosome 4 and two pairs of epistatic loci were detected in both field and pot experiments. One QTL on chromosome four was identified in both populations, and one marker locus RG323 on chromosome one was involved in additive effect in DH population, but epistasis effects in RI population.
We propose a phenomenological model for the intermediate scattering function (ISF) associated with density fluctuation in low temperature water. The motivation is twofold: to extract various physical parameters associated with the ISF computed from extended simple-point-charge model water at supercooled temperatures, and to apply this model to analyze high resolution inelastic x-ray scattering data of water in the future. The ISF of the center of mass of low temperature water computed from 10 M-step molecular dynamics (MD) data shows clearly time-separated two-step relaxation with a well-defined plateau in-between. We interpret this result as due to the formation of a stable hydrogen-bonded, tetrahedrally coordinated cage around a typical molecule in low temperature water. We thus model the long-time cage relaxation by the well-known Kohlrausch form exp[-(t/tau)(beta)] with an amplitude factor which is a k-dependent Debye-Waller factor A(k), and treat the short-time relaxation as due to molecular collisional motions within the cage. The latter motions can be described by the generalized Enskog equation, taking into account the confinement effect of the cage. We shall show that the effect of the confinement changes the collisional dynamics by modifying certain input parameters in the kinetic theory by a factor [1-A(k)](1/2). We solve the generalized Enskog equation approximately but analytically by a Q-dependent triple relaxation time kinetic model. This kinetic model was previously shown to account for the large k behavior of Rayleigh-Brillouin scattering from moderately dense, simple fluids. We find that our model fits well with the MD generated collective as well as single-particle ISFs. For the short-time collisional dynamics, we fix values of the hard sphere diameter sigma and pair correlation function at contact g(sigma), without introducing any adjustable parameters. The calculated ISFs reproduce the correct Brillouin peak frequencies at low k values. From the long-time dynamics, we deduce values of the Debye-Waller factor A(k), the Kohlrausch exponent beta(k), and the cage relaxation time tau(k).
X-linked agammaglobulinemia (XLA), an inherited disease, is caused by mutations in the Bruton's tyrosine kinase (BTK). The absence of functional BTK leads to failure of B-cell differentiation; this incapacitates antibody production in XLA patients, who suffer from recurrent, sometimes lethal, bacterial infections. BTK plays an important role in B-cell development; it interacts with several proteins in the context of signal transduction. Point mutation in the BTK gene that leads to deletion of C-terminal 14 aa residues of BTK SH3 domain was found in a patient family. To understand the role of BTK, we studied binding of BTK SH3 domain (aa 216-273, 58 residues) and truncated SH3 domain (216-259, 44 residues) with proline-rich peptides; the first peptide constitutes the SH3 domain of BTK, while the latter peptide lacks 14 amino acid residues of the C terminal. Proline-rich peptides selected from TH domain of BTK and p120cbl were studied. It is known that BTK TH domain binds to SH3 domains of various proteins. We found that BTK SH3 domain binds to peptides of BTK TH domain. This suggests that BTK SH3 and TH domains may associate in inter- or intramolecular fashion, which raises the possibility that the kinase may be regulating its own activity by restricting the availability of both its ligand-binding modules. We also found that truncated SH3 domain binds to BTK TH domain peptide less avidly than does normal SH3 domain. Also, we show that the SH3 and truncated SH3 domains bind to peptide of p120cbl, but the latter domain binds weakly. It is likely that the truncated SH3 domain fails to present to the ligand the crucial residues in the correct context, hence the weaker binding. These results delineate the importance of C-terminal in binding of SH3 domains and indicate also that improper folding and the altered binding behavior of mutant BTK SH3 domain likely leads to XLA.
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