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Biomedical subjects

C Ye

Publications and source records attributed to C Ye.

97 records · Page 6Linked to original sources
PubMed1992

Increased pulmonary artery elastolytic activity in adult rats with monocrotaline-induced progressive hypertensive pulmonary vascular disease compared with infant rats with nonprogressive disease.

In a rat model of pulmonary hypertension induced by monocrotaline, medial hypertrophy of the pulmonary arteries is associated with enhanced production (synthesis) of insoluble elastin relative to accumulation and an increased number of elastin fragments, features suggestive of an elastolytic process. In the present study, we measured and characterized pulmonary artery (PA) elastolytic activity at time points before as well as coincident with the progression of medial hypertrophy in monocrotaline-injected adult male Sprague-Dawley rats. We also determined whether medial hypertrophy is preceded by ultrastructural changes in elastin. Since medial hypertrophy develops but fails to progress in rats injected with monocrotaline at 8 days of age, we assessed whether, compared with adult rats, there were also structural and biochemical differences in elastin and elastolytic activity. A twofold increase in elastolytic activity per milligram tissue was observed 2 days after monocrotaline injection in adult rats (p less than 0.01), and there was an increased number of breaks in the internal elastic lamina (IEL) at 4 days (p less than 0.05) (i.e., before the development of medial hypertrophy). Associated with the progression of medial hypertrophy between 16 and 28 days after monocrotaline injection, there was a further threefold increase in elastolytic activity per milligram tissue by 28 days (p less than 0.01). Susceptibility of the elastolytic activity to specific inhibitors suggested that one or more serine elastases is involved. In infant rats in which medial and right ventricular hypertrophy fail to progress in severity between 16 and 28 days after monocrotaline injection, we did not measure an increase in elastolytic activity, nor was there evidence of an increase in the number of breaks in the IEL at 4 days, suggesting a lack of increased elastolytic activity at an earlier time point. The total content of PA elastin in infant rats, although increased compared with control rats (p less than 0.01), was not associated with heightened production and appeared ultrastructurally as thicker laminae (p less than 0.05) rather than as fragments previously reported in adult rats.

Age Factors↗
PubMed1992

New developments in the pulmonary circulation in children.

This article reviews new advances in the diagnosis, treatment, and pathophysiology of pulmonary hypertension. In diagnosis, Doppler echocardiography has been refined and its accuracy increased, and magnetic resonance imaging is being developed as a new tool. Prostacyclin and calcium channel blockers remain mainstays of short-term therapy, and single-lung transplantation has become a new therapeutic option. Newer pharmacotherapeutics are largely directed at reversing acute vasoconstriction and include approaches related to ATP and endothelial-derived relaxing factor. In pathophysiology, recent studies have shown the role of elastase in mediating the initiation and progression of pulmonary hypertension, the regulation of gene expression of collagen, elastin, endothelin, and growth factors including transforming growth factor-B and insulinlike growth factor-I, and mechanisms of signal transduction of smooth muscle cell proliferation. The migratory smooth muscle cell phenotype has been studied in terms of altered expression of endothelial and smooth muscle extracellular matrix components, including hyaluronan and fibronectin, respectively.

Child↗
PubMed1991

[Pharmacological study on an irreversible ligand of opioid receptors, A-alpha-CAM, and its reaction with the sulfhydryl group].

A-alpha-CAM is an irreversible partial agonist of opiate receptors. Its binding to opioid receptors from P2 membrane preparations of rat brain and its effect on isolated tissues (GPI, MVD, RVD and RbVD) could not be washed away, indicating the irreversible nature of its binding. A-alpha-CAM inhibited the electrically elicited contraction of GPI, a pure agonist, with an IC50 of 2.6 mumol/L, and this effect could not be antagonized by Nx. A-alpha-CAM acted as a partial agonist on MVD with an IC50 of 0.153 mumol/L. With RVD and RbVD, A-alpha-CAM acted as an antagonist with PA2 values of 7.4 and 7.7 respectively. Possibly, covalent binding of A-alpha-CAM with the sulfhydryl groups of opioid receptors is the biochemical mechanisms responsible for its irreversible action.

Animals↗
PubMed1989

Plasmid expression and maintenance during long-term starvation-survival of bacteria in well water.

Strains of enteric bacteria and pseudomonads containing plasmid R388::Tnl721 (Tpr, Tcr) or pRO101 (Hgr, Tcr) were starved for over 250 days in sterile well water to evaluate effects of starvation-survival on plasmid expression and maintenance. Viable populations dropped to between approximately 0.1 and 1% of the initial populations. Escherichia coli(pRO101) and Pseudomonas cepacia(pRO101) lost both viability and plasmid expression at a lower rate than strains containing R388::Tnl721. Three patterns of host-plasmid interaction were detected: (i) no apparent loss of plasmid expression, (ii) loss of plasmid expression on initial recovery with subsequent expression upon resuscitation, and (iii) loss of capability to produce functional plasmid resistance.

Culture Media↗
PubMedDate not supplied

Oral HCFU for prevention of hepatic metastasis in an experimental colorectal carcinoma.

BACKGROUND: An animal experimental study was performed to clarify the preventive effect of oral HCFU (1-hexylcarbamoyl-5-fluorouracil) on hepatic metastasis. MATERIALS AND METHODS: A total of 180 BALB/C mice received intraportal injection of Colon26 cancer cells followed by oral daily administration of HCFU to 5 groups divided according to the dose of HCFU, ranging from 0 (controls) to 7.5 mg/Kg for 21 days. These groups were independently assigned to 3 sets to investigate survival rates and degree of hepatic metastasis. RESULTS: Mean survivals in the treated groups were statistically longer than the controls except the 7.5 mg/Kg group. HCFU 7.5 and 5 mg/Kg groups showed the minimum liver weight on the 12th and 16th day, respectively these were significantly smaller than those of controls (p < 0.0001 in both). CONCLUSION: It is considered that oral HCFU could effectively suppress hepatic metastasis of the colon26 cancer cells and prolong the life of animals. This finding suggests the promising clinical application of oral HCFU for colorectal cancer after curative resection.

Administration, Oral↗