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Biomedical subjects

C Yee

Publications and source records attributed to C Yee.

68 records · Page 4Linked to original sources
PubMed1981

Synthesis of pro-collagen type II by a xenotransplanted human chondroblastic osteosarcoma.

The chondroblastic component of a human osteosarcoma was established as a transplantable tumor in nude athymic mice for the first time. The collagen biosynthetic production of the excised tumor was studied in organ culture. The majority of the collagen secreted in the culture media was pro-Type II as identified by diethylaminoethyl cellulose (DEAE cellulose) chromatography, gel electrophoresis, and cyanogen bromide cleavage. This system should be useful for producing large quantities of human pro-Type II collagen and for studying neoplastic cell switching of collagen synthesis.

Adult↗
PubMed1978

Characteristics of the Fc receptor induced by herpes simplex virus.

Studies of the Fc receptor induced by herpes simplex virus using the 7S EA rosette assay reveal that (i) the percentage of cells showing receptor activity is multiplicity-dependent; (ii) the percentage of cells rosetting is independent is independent of the cell cycle phase of the host cell; (iii) RNA and viral protein synthesis, and probably glycosidation, are required for the induction of the receptor but not viral DNA synthesis; and (iv) the receptor is neuraminidase-resistant but trypsin-sensitive.

Binding Sites, Antibody↗
PubMed1977

Oncogenicity of a nude mouse cell line transformed by a human papovavirus.

Primary cultures of NIH nude mouse (nu/nu) kidney cells were transformed with a human papovavirus (MMV). The transformed cell line expressed T-antigen, and MMV DNA was found to be associated with the cell DNA. When NIH nu/nu mice were inoculated with the transformed cells, they developed tumors at the injection site but failed to generate detectable levels of T-antibody. A control group of nu/+ littermates rejected the tumor inoculum but mounted an antibody response to T-antigen. It was proposed that nude mouse cells may be a suitable system to test oncogenicity of in vitro transformed cells.

Animals↗
PubMed1976

Hamster ependymomas produced by intracerebral inoculation of a human papovavirus (MMV).

Ependymomas were produced in 3 of 11 newborn hamsters inoculated intracerebrally with a human papovavirus (MMV) isolated from a malignant lymphoma of the brain of a child with Wiskott-Aldrich syndrome. One of the tumors was serially transplanted into weanling hamsters. Cells from the transplanted tumors and from cell cultures derived from these tumors contained an intranuclear "T" antigen that reacted with simian virus 40 T antibody.

Animals↗
PubMed1976

Automated analysis of antigen-stimulated lymphocytes.

Human peripheral blood lymphocytes were stimulated with phytohemagglutinin and tuberculin-purified protein derivative to determine if flow microfluorometric techniques had the sensitivity to detect high and low levels of blastogenic response. The deoxyribonucleic acid content of the lymphocytes was analyzed after culture. Blastogenic response was found to be measurable both sensitively and reproducibly. Tritiated thymidine incorporation measurements were also made. Preliminary experiments using inactivated Herpesvirion (type I) as a stimulant showed blastogenic response detectable by flow microfluorometric deoxyribonucleic acid measurements. Refinements of this technique may prove to be useful in the study of lymphocyte response to viral antigens in patient populations.

Autoanalysis↗
PubMed1975

Evaluation of the lymphocyte blastogenic response by rapid flow analysis.

The data presented show that the blastogenic response of human peripheral lymphocytes to mitogens and specific antigens can be easily evaluated with rapid flow analysis of mithramycin stained cells. The precision, reproducibility and rapidity of the method make it highly suitable to study lymphocyte response to antigens in patient populations.

Cell Division↗
PubMed1975

Antibodies to herpesviruses in patients with cervical cancer and controls.

The indirect immunofluorescent test was used to detect antibodies to herpesviruses types 1 and 2, cytomegalovirus and EBV in sera from patients with cervical carcinoma, condyloma acuminatum and controls from Cali, Colombia, and a control group from the USA. No significant differences were found in the prevalence of antibodies to viral capsid antigens of HSV-1 and CMV among the groups studied. However, titres for HSV-2 were higher in the three groups from Cali (cervical carcinoma, condyloma and controls) than in the control groups from USA. High EBV antibody titres (VCA and early antigens) were found in the three groups from Cali. Antibodies to early antigens of CMV were detected in a sub-sample of the subjects and with higher frequency in patients with cervical cancer. The significance of these findings is discussed.

Antibodies, Viral↗
PubMedDate not supplied

Vitamin A inhibits some aspects of systemic disease due to local x-radiation.

We have previously reported that supplemental vitamin A ameliorates the stress response to a wide variety of noxious agents. The present study was carried out to determine how supplemental vitamin A influences the course of radiation sickness in C3H female mice subjected to 3000 R irradiation of one lower hind limb. All mice ingested a chow diet containing about 13,000 units of vitamin A/kg diet (about half as preformed vitamin A and half as beta-carotene) which supports normal growth, development, and reproduction of normal mice. One hundred fifty thousand units of vitamin A/kg chow was added for the vitamin A supplemented mice. All mice ate and drank ad libitum. The supplemental vitamin A feeding was begun either 3 days before radiation or immediately after radiation. There were no significant differences in the effects of these two regimens. The supplemental vitamin A prevented the weight loss, moderated the adrenal hypertrophy, prevented the thymic involution, and lessened the lymphopenia due to radiation. We conclude that supplemental vitamin A has both prophylactic and therapeutic benefits in radiation-induced disease.

Adrenal Glands↗
PubMedDate not supplied

Unusual intranuclear inclusions in malignant fibrous histiocytoma: presence in primary tumor, metastases, and xenografts.

We describe previously unreported intranuclear inclusions in 2 cases of malignant fibrous histiocytoma. The inclusions were found in 2-10% of the tumor cells removed from the patients and in 2-10% of the cells examined in tumor tissue xenotransplanted in nude mice. By stereo electron microscopy the inclusions are closely packed undulating fibrils 18-23 nm in diameter. They are sometimes associated with fibrillary bodies. They closely resemble the inclusions reported in some animals inoculated with serum from patients with non-A non-B hepatitis; however, their nature at present is unknown.

Adult↗