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C Zalut

Publications and source records attributed to C Zalut.

At least 19 recordsLinked to original sources
PubMed1980

Micro quantitative Edman manual sequencing.

A manual Edman technique is described which allows sequential quantitative determination of from 3 to 10 amino terminal residues on quantities of peptides or proteins down to one nanomole. This is achieved by a fast, efficient method of obtaining the anilinothiazolinone or phenylthiohydantoin amino acid, and quantitating by either back hydrolysis and amino acid analysis or by a new, rapid, high resolution, quasi-isocratic, high-pressure liquid chromatographic procedure. The overall method has been extensively tested successfully on both peptides and proteins of known and unknown amino-terminal sequence and the results included here. In addition, a wide variety of applications relevant to primary structure analysis such as sequencing blocked polypeptides, use of denaturing agents as coupling buffers, reduction of protein or peptide losses on consecutive sequencing and peptide mixture analysis are all incorporated in the methodology outlined.

Amino Acid Sequence↗
PubMed1980

A high-speed, quasi-isocratic high-pressure liquid chromatography system for protein sequence analysis.

A simple, of less than 20 min duration, quasi-isocratic high-pressure liquid chromatographic system designed for effective analysis of phenylthiohydantoin-amino acids derived from Edman sequencing is described. A second 30-min quasi-isocratic system is presented which may be used when sequenced samples contain extraneous 254 nm-absorbing material. Conditions affecting chromatogram reproducibility are also examined.

Amino Acid Sequence↗