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Biomedical subjects

C van Peteghem

Publications and source records attributed to C van Peteghem.

7 recordsLinked to original sources
PubMed1999

Development of a simple method for the determination of genistein, daidzein, biochanin A, and formononetin (biochanin B) in human urine.

A simple method was developed for the determination of free and/or total isoflavones daidzein, genistein, and their respective 4'-methoxy derivatives biochanin A and formononetin (biochanin B) at low levels in human urine. A solid-phase extraction on octadecyl silica (C(18)) columns was used for the isolation of the phytoestrogens from the matrix. An extraction on a ChemElut 1010 column connected on-line to a Florisil cartridge by a Teflon stopcock was used for effective eluate purification. A mixture of dichloromethane and ethyl acetate was used for elution of the isoflavones from the columns in tandem. The isoflavones were determined as trimethylsilyl (TMS) ethers using GC/MS-SIM after separation on an HP-5MS fused silica column. TMS ethers were obtained by using BSTFA containing 1% of TMCS. For the determination of free isoflavones 6-hydroxyflavone was used as internal standard, whereas robigenin was used in the case of total isoflavone determination. Recoveries for free isoflavones under study varied from 63.5 to 89.6% at the 25 ng mL(-)(1) level and from 63.5 to 89. 2% at the 5 ng mL(-)(1) level in urine. Analytical curves were linear between 5 and 25 ng mL(-)(1). Detection limits varied from 1 ng mL(-)(1) for formononetin to 2.3 ng mL(-)(1) for daidzein. Recoveries for total isoflavone determination after enzymatic hydrolysis with glucuronidase from Helix pomatia ranged from 56.5 to 77.1% at the 25 ng mL(-1) level.

Anticarcinogenic Agents↗
PubMed1991

Survey of chemical exposures in a viscose rayon plant.

Measurements of carbon disulphide and hydrogen sulphide in a Belgian viscose rayon plant, using personal monitoring with charcoal tubes for carbon disulphide and impingers for hydrogen sulphide, showed considerable differences in the average exposure for the different jobs (3-147 mg m-3 for carbon disulphide and 0-9 mg m-3 for hydrogen sulphide). Air pollutants such as mercury and sulphuric acid, which have been reported in some other studies, were not present. The lead in blood concentration found in four plumbers ranged from 28 to 49 micrograms 100 ml-1. The implications of these findings for industrial hygiene and epidemiology are discussed.

Air Pollutants, Occupational↗
PubMed1990

Accumulation of carbon disulphide metabolites.

Biological monitoring for carbon disulphide (CS2) exposure performed using the iodine-azide test (IAT) and 2-thiothiazolidine-4-carboxylic acid (TTCA) test in urinalysis of workers with high exposure to CS2 (112-142 mg/m3, n = 34), workers with low exposure (4-7 mg/m3, n = 16), and non-exposed university workers (n = 10). Pre-shift and post-shift urine specimens were collected on three consecutive days in the exposed and for only one day in the non-exposed. According to the findings the specificity and the sensitivity seem to be low for the IAT and high for the TTCA test. Contrary to a previous report all pre-shift urine samples showed negative IATs. The TTCA test was positive in pre-shift urine even after 32 to 63.5 h without exposure, and values tended to increase during consecutive days of exposure in highly exposed workers. The possible health implications of these findings should be further investigated.

Air Pollutants, Occupational↗
PubMed1989

Quantitative analysis of anabolics on thin-layer chromatographic plates using image analysis techniques.

A new detection system is introduced for the quantitative analysis of thin-layer chromatographic plates, which is based on a relatively simple, cheap but advanced image analysis system. Both one- and two-dimensional plates can be analysed. Recording and analysis can also be performed from photographs or even slides. Applications are shown for a number of samples containing anabolic compounds.

Anabolic Agents↗
PubMed1989

Thin-layer chromatographic method for the detection of anabolics in fatty tissues.

A modification of the method of Verbeke [J. Chromatogr., 177 (1979) 69] is presented. The fatty tissue is dissolved in hexane, partitioned against methanol-sodium acetate buffer (pH 5.2) and extracted with dichloromethane. The crude extract is then purified on a disposable C18 column. The final extract together with a set of reference compounds is spotted on two opposite sides of a high-performance thin-layer chromatographic plate (10 X 10 cm), which is developed with two different eluents in two opposite directions. This mode of operation has the advantage that more reference compounds can be spotted and that the identification is based on two independent chromatographic runs. Also the total analysis time is decreased.

Adipose Tissue↗
PubMed1989

Chemiluminescence immunoassay for the detection and quantification of methyltestosterone residues in muscle tissue.

Chemiluminescence as a detection method for immunoassay has successfully been applied to the measurement of methyltestosterone (MT) residues in muscle tissue. The sample is digested enzymatically, extracted with diethyl ether and purified on a Lipidex-5000 column. An optional clean-up utilized disposable C18 columns. As the luminescent label the N-(4-aminobutyl)-N-ethylisoluminol conjugate of MT was used. The antiserum was raised in a rabbit against MT-3-carboxymethyloxime-bovine serum albumin. The detection limit of the assay was 14 +/- 7 pg (n = 13), with a limit of quantification in muscle tissue of 0.125 ppb.

Animals↗