Biomedical subjects

Catherine Amiel

Publications and source records attributed to Catherine Amiel.

6 recordsLinked to original sources

PubMed2006

New self-assembled nanogels based on host-guest interactions: characterization and drug loading.

We show here, for the first time, that two neutral polymers may completely associate together in water to spontaneously form supramolecular nanoassemblies (nanogels) of spherical shape. The cohesion of these stable structures of about 200 nm is based upon a "lock and key" mechanism: inclusion complexes are formed between the hydrophobic alkyl chains grafted on a polysaccharide (dextran) and the molecular cavities contained in a poly-cyclodextrin polymer. Production yields reached 95%. It was established that all the alkyl chains were included within the cyclodextrins' cavities in these nanoassemblies. The multivalent character of the interactions between the two polymers ensures the stability of the nanoassemblies. Moreover, empty cyclodextrin units remained accessible for the inclusion of compounds of interest such as benzophenon or tamoxifen.

Antineoplastic Agents, Hormonal↗

PubMed2006

Rheological and structural characterization of the interactions between cyclodextrin compounds and hydrophobically modified alginate.

Interactions in semidilute solutions of a hydrophobically modified alginate (HM-alginate) in the presence of hydroxypropyl-beta-cyclodextrin (HP-beta-CD) monomer or a beta-cyclodextrin polymer (poly(beta-CD)) have been characterized at different temperatures with the aid of rheology and small-angle neutron scattering (SANS). The viscosity results for the HM-alginate/HP-beta-CD system reveal progressive deactivation of the hydrophobic associations as the concentration of HP-beta-CD increases. For the HM-alginate/poly(beta-CD) system, on the other hand, addition of poly(beta-CD) sets up bridges between adjacent polymer chains and thereby strengthens the associative network. A novel shear-thickening effect is observed at fairly high shear rates for the HM-alginate/poly(beta-CD) system, and this feature is influenced by temperature. Elevated temperature induces higher chain mobility and the formation of weaker network associations. Analyses of the SANS data disclosed that the association strength in HM-alginate/poly(beta-CD) mixtures increases strongly with increasing cosolute concentration, whereas no effect or a moderate weakening of the strength can be traced in HM-alginate/HP-beta-CD solutions upon addition of HP-beta-CD. The value of the correlation length xi is virtually not affected by the addition of cosolute for the HM-alginate/poly(beta-CD) system, whereas the decoupling of hydrophobic moieties of the polymer upon addition of HP-beta-CD gives rise to a smaller value of xi, suggesting that the size of the heterogeneity patches is reduced. The SANS results suggest that compact association structures are formed in the HM-alginate/poly(beta-CD) solutions.

Alginates↗

PubMed2006

Macromolecular assemblies based on coupled inclusion complex and electrostatic interactions.

Macromolecular assemblies are elaborated by mixing beta-cyclodextrin-containing polymer (polybetaCD), dextran sulfate polyanion (NaDxS), and cationic amphiphiles which are adamantane derivatives (Ada1 or Ada2) in aqueous medium. These components are assembled via coupled inclusion complex interactions (adamantyl group with cyclodextrin cavity) and electrostatic attractive interactions (positive charges of Ada with negative charge of NaDxS). The structural properties are studied by viscometry and small angle neutron scattering. Ternary aggregates with larger size and lower compacities are observed as the cation concentration is increased, until phase separation occurs. The results are in good agreement with a core-shell association mechanism, the core being made of one polybetaCD chain, the shell of NaDxS chains, and the Ada amphiphiles being distributed more or less homogeneously inside the cyclodextrin cavities. The nature of the Ada counterions has a strong influence on the association as Ada1 with I(-) counterions give smaller and less compact aggregates than Ada2 with Br(-) counterions.

Binding, Competitive↗

PubMed2005

Ternary complex formation in aqueous solution between a beta-cyclodextrin polymer, a cationic surfactant and DNA.

Polyelectrolyte complexes have been elaborated by mixing in water neutral poly(beta-CD), a cationic surfactant (DTAC) and herring sperm DNA fragments. The driving forces for the poly(beta-CD)/DTAC/DNA association in aqueous solution are, on the one hand, reversible inclusion interactions between the CD cavities of poly(beta-CD) and the alkyl group of DTAC, leading to the formation of a polycation and, on the other hand, electrostatic interactions between the opposite charges of the cationic surfactant and anionic DNA. Viscometry and SANS have been used to prove the occurrence of such ternary complexes in dilute aqueous solutions.

Carbohydrate Sequence↗

PubMed2005

Associative pH sensitive system.

New associative pH sensitive systems composed of a modified dextran bearing 2-carboxycyclohexyl carboxyl groups and neutral (p betaCD) or positively charged (p betaCDN(+))beta-cyclodextrin-co-epichlorhydrin copolymers have been synthesized and their properties were investigated as a function of pH by phase diagrams and viscosimetry. The affinity between the CD cavities and the hydrophobic guests (2-carboxycyclohexyl carboxyl groups) has been studied as a function of pH. The system with the neutral p betaCD shows a pH dependent behavior due to the lowest affinity of the guest at pH above 5 for the cavity. Associative phase separation is produced at low pH whereas soluble complexes occur at higher pH. The opposite trend is observed with the system containing the cationic p betaCDN(+). This behavior is due to the combination of electrostatic and inclusion complex interactions in this system. Addition of salt, by screening the electrostatic interactions, also strongly influences the response of the system.

Dextrans↗

PubMed2004

Polymer bilayer formation due to specific interactions between beta-cyclodextrin and adamantane: a surface force study.

The purposes of this study are to utilize the interactions between an adamantane end-capped poly(ethylene oxide) (PEO) and a cationic polymer of beta-cyclodextrin to build polymer bilayers on negatively charged surfaces, and to investigate the interactions between such layers. The association of this system in solution has been studied by rheology, light scattering, and fluorescence measurements. It was found that the adamantane-terminated PEO (PEO-Ad) mixed with the beta-cyclodextrin polymer gives complexes where the interpolymer links are formed by specific inclusion of the adamantane groups in the beta-cyclodextrin cavities. This results in a higher viscosity of the solution and growth of intermolecular clusters. The interactions between surfaces coated with a cationized beta-cyclodextrin polymer across a water solution containing PEO-Ad polymers were studied by employing the interferometric surface force apparatus (SFA). In the first step, the interaction between mica surfaces coated with the cationized beta-cyclodextrin polymer in pure water was investigated. It was found that the beta-cyclodextrin polymer adsorbs onto mica and almost neutralizes the surface charge. The adsorbed layers of the beta-cyclodextrin polymer are rather compact, with a layer thickness of about 60 A (30 A per surface). Upon separation, a very weak attractive force is observed. The beta-cyclodextrin solution was then diluted by pure water by a factor of 3000 and a PEO-Ad polymer was introduced into the solution. Two different architectures of the PEO-Ad polymer were investigated: a four-arm structure and a linear structure. After the adsorption of the PEO polymer onto the beta-cyclodextrin layer reached equilibrium, the forces were measured again. It was found that the weak repulsive long-range force had disappeared and an attractive force caused the surfaces to jump into contact, and that the compressed layer thickness had increased. The attractive force is interpreted as being due to a specific recognition between the hydrophobic adamantane groups on the PEO-Ad polymer and the hydrophobic cavity in the beta-cyclodextrin molecules. Furthermore, the attractive force observed on separation has increased significantly, which is a further indication of a specific interaction between the beta-cyclodextrin polymer and the adamantane groups.

Adamantane↗