Biomedical subjects

Catherine Charcosset

Publications and source records attributed to Catherine Charcosset.

4 recordsLinked to original sources

PubMed2006

Membrane processes in biotechnology: an overview.

Membrane processes are increasingly reported for various applications in both upstream and downstream technology, such as the established ultrafiltration and microfiltration, and emerging processes as membrane bioreactors, membrane chromatography, and membrane contactors for the preparation of emulsions and particles. Membrane systems exploit the inherent properties of high selectivity, high surface-area-per-unit-volume, and their potential for controlling the level of contact and/or mixing between two phases. This review presents these various membrane processes by focusing more precisely on membrane materials, module design, operating parameters and the large range of possible applications.

Bioreactors↗

PubMed2006

Influence of the formulation for solid lipid nanoparticles prepared with a membrane contactor.

Solid lipid nanoparticles (SLN) were introduced in the 1990s as an alternative to microemulsions, polymeric nanoparticles, and liposomes. The SLN are reported to have several advantages, i.e., their biocompatibility and their controlled and targeted drug release. In this paper, we present a new process for the preparation of SLN using a membrane contactor to allow large scale production. The lipid phase is pressed, at a temperature above the melting point of the lipid, through the membrane pores allowing the formation of small droplets. The lipid droplets are then detached from the membrane pores by the aqueous phase flowing tangentially to the membrane surface. The SLN are formed by the following cooling of the preparation below the lipid melting point. The influence of the aqueous phase and lipid phase formulations on the lipid phase flux and on the SLN size are studied. It is shown that SLN are obtained with a lipid phase flux between 0.21 and 0.27 m3/h.m2, SLN size between 175 and 260 nm. The advantages of this new process are demonstrated to be its facility of use and its scaling-up ability.

Algorithms↗

PubMed2005

Preparation of solid lipid nanoparticles using a membrane contactor.

Solid lipid nanoparticles (SLN) were introduced at the beginning of the 1990s, as an alternative to solid nanoparticles, emulsions and liposomes in cosmetic and pharmaceutical preparations. The present study investigates a new process for the preparation of SLN using a membrane contactor. The lipid phase is pressed, at a temperature above the melting point of the lipid, through the membrane pores allowing the formation of small droplets. The aqueous phase circulates inside the membrane module, and sweeps away the droplets forming at the pore outlets. SLN are formed by the following cooling of the preparation to room temperature. The influence of process parameters (aqueous phase and lipid phase temperatures, aqueous phase cross-flow velocity and lipid phase pressure, membrane pore size) on the SLN size and on the lipid phase flux is investigated. It is shown that the membrane contactor allows the preparation of SLN with a lipid phase flux between 0.15 and 0.35 m3/h m2, and a mean SLN size between 70 and 215 nm. The advantages of this new process are its facility of use, the control of the SLN size by an appropriate choice of process parameters, and its scaling-up abilities.

Drug Compounding↗

PubMed2005

A new process for drug loaded nanocapsules preparation using a membrane contactor.

In this paper, we describe a new process for the preparation of drug loaded nanocapsules using a membrane contactor which may be scaled up for industrial applications. Nanocapsules are prepared according to the nanoprecipitation method. The organic phase (solvent, polymer, oil, and drug) is pressed through the pores of an ultrafiltration membrane via the filtrate side. The aqueous phase (water and surfactant) circulates inside the membrane module, and sweeps away the nanocaspules forming at the pore outlets. Two model drugs are selected for the preparation of drug loaded nanocapsules: indomethacin and vitamin E. It is shown that indomethacin loaded nanocapsules with a mean diameter of 240 nm and vitamin E loaded nanocapsules with a mean diameter of 230 nm are obtained with a 150,000 daltons ultrafiltration membrane, a transmembrane pressure of 3 bar, and a crossflow rate of 1.7 m.s(- 1). High fluxes are also obtained (around 0.6 m3/h.m2), leading to the preparation of 1.8 10(- 3) m3 drug loaded nanocapsules in 8 min. The advantage of this membrane contactor compared to other processes for drug loaded nanocapsules preparation is shown to be its scale-up ability.

Anti-Inflammatory Agents, Non-Steroidal↗