Catherine E Willett | PubMed Health

PubMed2004

Expression of the winged helix/forkhead gene, foxn4, during zebrafish development.

The winged-helix/forkhead transcription factor gene, foxn4, is expressed in the nervous system of developing and adult zebrafish. Prominent expression sites include the olfactory placode, the basal layer of the olfactory epithelium, the neuroepithelium of the developing retina, the germinal zone of the differentiated eye, regions of motoneuron development in the neural tube and periventricular regions of the brain. The adult thymus is the only major site of foxn4 expression outside of the nervous system.

Amino Acid Sequence↗

PubMed2004

T cells and the thymus in developing zebrafish.

The expression of genes encoding T cell receptor (TCR) alpha was used to follow the development of the thymus and to analyze the distribution of T cells in zebrafish. In the thymus, expression was first detected, by in situ hybridization, at four days post fertilization. In RNA extracted from whole fish, TCRalpha transcripts were also detected at four days and reached adult levels at three weeks. At six weeks, TCRalpha was expressed throughout the thymus, whereas rag1 expression was localized to the peripheral regions. Expression of TCRalpha outside the thymus was detected at nine days. In adult peripheral organs, the greatest expression was in the pronephros, mesonephros and intestine; expression in the spleen became greater as fish age. Three new, relatively highly expressed, TCR Valpha families were identified.

Amino Acid Sequence↗