Biomedical subjects
Publications and source records attributed to Chang-xin Wu.
SSCP analysis was commanded on the whole sequence of porcine LHbeta gene except the upstream control region. No polymorphic site was found in the 3 exons and 3' control region. But there were 2 polymorphic sites in the 1367 site of intron 1 and 1823 site of intron 2. The gene frequencies of the 2 sites in the Erhualian,Yorkshire and Landrace populations were analyzed.
The recognizing sequence of restriction enzyme includes palindrome and nonpalindromic, and DNA is double helix complementary strands. So the recognizing sequences of palindrome enzyme in two strands of DNA were identical,and can be considered of one sequence. But for nonpalindromic restriction enzyme,the recognizing sequences of two strands of DNA were not identical. Therefore the true recognizing sequences are not only one. In this experiment,an enzyme cleavage reaction was carried out which confirmed that the true recognizing sites/sequences of nonpalindromic enzyme are two instead of one.
As the molecular biology has been applied in animal genetics and breeding,it is important that we research major genes on quantitative traits for animal breeding by transgenic technology. In this paper,the research advance of FABP genes, obese gene, leptin gene, MCRs genes, LPL gene, HSL gene affecting meat quality in animals are reviewed.