PharmaDiscovery: closing the productivity gap.
There is no doubt that a more efficient dissemination of scientific knowledge and the removal of drug discovery silos will aid the deepening productivity crisis.
Biomedical Research↗
Biomedical subjects
Charlotte Carey
Publications and source records attributed to Charlotte Carey.
Single-molecule detection technologies in miniaturized high-throughput screening: fluorescence intensity distribution analysis.
Single-molecule detection technologies are becoming a powerful readout format to support ultra-high-throughput screening. These methods are based on the analysis of fluorescence intensity fluctuations detected from a small confocal volume element. The fluctuating signal contains information about the mass and brightness of the different species in a mixture. The authors demonstrate a number of applications of fluorescence intensity distribution analysis (FIDA), which discriminates molecules by their specific brightness. Examples for assays based on brightness changes induced by quenching/dequenching of fluorescence, fluorescence energy transfer, and multiple-binding stoichiometry are given for important drug targets such as kinases and proteases. FIDA also provides a powerful method to extract correct biological data in the presence of compound fluorescence.
Alcohol Dehydrogenase↗