Biomedical subjects
Publications and source records attributed to Chengde Mao.
This paper reports a novel DNA six-point-star motif assembled from only three different DNA single-strands. This motif readily assembles into hexagonal two-dimensional arrays with high connectivity. Such a high connectivity could potentially improve the array stability.
Two types of DNA star motifs (tiles) can recognize and associate with like tiles to form 2D arrays but exclude unlike tiles even though the local interactions between any two tiles are exactly the same.
We have developed a strategy for preparing antibody nanoarrays by DNA templating. In the resulting tetragonal antibody arrays, each spot is a similarly orientated, individual antibody molecule (IgG). The separation between two adjacent IgG molecules is only approximately 20 nm.
Branched structures with long DNA duplex arms have been constructed through biotin-streptavidin binding and characterized by gel electrophoresis and atomic force microscopy (AFM) imaging.
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A subtle balance of flexibility and stress is found to be critical for a DNA nanostructure to be a good self-assembly block.
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A three-point-star DNA motif has been designed and constructed, which can self-assemble into hexagonal two-dimensional lattices. The resulting lattices are up to 1 mm.
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A combination of a DNA ligase and a restriction endonuclease provides a DNA polymerase activity, which might suggest a novel strategy for polymer synthesis.
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We present a designed cyclic DNA motif that consists of six DNA double helices that are connected to each other at two crossover sites. DNA double helices with 10.5 nucleotide pairs per turn facilitate the programming of DNA double crossover molecules to form hexagonally symmetric arrangements when the crossover points are separated by seven or fourteen nucleotide pairs. We demonstrate by atomic force microscopy well-formed arrays of hexagonal six-helix bundle motifs both in 1D and in 2D.
The use of DNA as programmable building blocks to achieve highly-definable nanostructures, termed as DNA nanotechnology, has offered great opportunities to control matter at a very small scale. Fast growing researches along this direction has led to the emergence of several significant branches of DNA nanotechnology. This review intends to summarize the recent developments in this area. Several topics will be discussed with a focus on the works from the authors' group, including (1) DNA self-assembly and DNA-directed self-assembly, (2) DNA nanomachines, (3) DNA-based electronics, (4) DNA assisted nanofabrications, and (5) DNA computations.
A strategy has been developed for switching chemical reactions between two identical reagents on the basis of DNA duplex-triplex transition. In response to the change of solution pH, a DNA complex changes its conformation and repositions chemical reagents that are conjugated with DNA strands. As a result, chemical reactions are reprogrammed. This strategy is expected to be applicable to sophisticated chemical syntheses.
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A pair of DNA nanocircles has been constructed to model gears. The nanogears can move against each other continuously. Each gear consists of a DNA duplex circle and three single-stranded teeth. The teeth on the two gears are brought together by linker strands, which are complementary to the gear teeth. The teeth are separated from each other by removal of the linker strands with a strand displacement mechanism.