Biomedical subjects

Christian David

Publications and source records attributed to Christian David.

7 recordsLinked to original sources

PubMed2007

Extraordinary optical transmission in the ultraviolet region through aluminum hole arrays.

We investigated the extraordinary optical transmission phenomenon in the UV range by fabricating large-area, free-standing aluminum hole arrays using extreme UV interference lithography and shadow thermal evaporation. Transmission spectra show strong peaks in the UV region resulting from both surface plasmon polariton and localized surface plasmon excitations. The results indicate that the high plasmon frequency of Al is directly responsible for the presence of strong resonance peaks in the UV region, which supports the role of plasmonic phenomena in the extraordinary transmission. The simple fabrication method enables large-area production of such structures for research and industrial production purposes.

Journal Article↗

PubMed2006

Growth cone navigation in substrate-bound ephrin gradients.

Graded distributions of ephrin ligands are involved in the formation of topographic maps. However, it is still poorly understood how growth cones read gradients of membrane-bound guidance molecules. We used microcontact printing to produce discontinuous gradients of substrate-bound ephrinA5. These consist of submicron-sized protein-covered spots, which vary with respect to their sizes and spacings. Growth cones of chick temporal retinal axons are able to integrate these discontinuous ephrin distributions and stop at a distinct zone in the gradient while still undergoing filopodial activity. The position of this stop zone depends on both the steepness of the gradient and on the amount of substrate-bound ephrin per unit surface area. Quantitative analysis of axon outgrowth shows that the stop reaction is controlled by a combination of the local ephrin concentration and the total amount of encountered ephrin, but cannot be attributed to one of these parameters alone.

Animals↗

PubMed2005

X-ray beam-position monitoring in the sub-micrometre and sub-second regime.

It is demonstrated that X-ray beam positions can be extracted from two-dimensional profiles with sub-pixel resolution. Beam-position measurements utilizing a self-designed low-cost two-dimensional detector have been performed at two synchrotron radiation beamlines of the Swiss Light Source. The effective detector pixel size was 4.8 microm and the resolution achieved for the beam position was about 5 nm. At a data rate of 25 frames per second, periodic variations of the beam position could be detected with a frequency resolution below 0.1 Hz. This allowed, for example, the influence of a turbo-pump in the X-ray optics hutch on the beam position to be quantified, and even minute variations related to the electron beam in the storage ring could be detected.

Journal Article↗

PubMed2004

The partial Talbot effect and its use in measuring the coherence of synchrotron X-rays.

The Talbot effect is the self-imaging, at distances D multiple of D(R), of the intensity downstream of a periodic object. Earlier work with hard synchrotron radiation X-rays showed the variation with D of the fundamental Fourier component of intensity to be a good measurement of beam coherence. Any higher-order Fourier coefficients I (D, m > 1) would be periodic with a reduced period D(Rm) = D(R)/m for an ideally coherent incident beam (partial Talbot effect). The degree of coherence gamma(x) is sampled through the ratio of I (D, m) at D = 0 and multiples of D(Rm). This requires the Fourier coefficient for D = 0, which is not accessible for a phase object (no contrast at D = 0). However, the ratio of the slopes of I (D, m) at D = 0 and D = pD(Rm) also provides this information. Furthermore, a characterization of gamma(x) is possible, provided an assumption is made on its shape, using only the ratio of the Fourier coefficient I (D, m) of two images a distance pD(Rm) apart. Experiments with one- and two-dimensional phase gratings and a mixed (amplitude and phase) two-dimensional grating confirm that the partial Talbot effect approach is viable. It requires a reduced range of distances, and yields important results directly, obviating the need for computer fits. In particular, 8% of the beam intensity was found to have very low coherence in the vertical direction, probably due to monochromator imperfection.

Algorithms↗

PubMed2004

Cell behaviour on micropatterned substrata: limits of extracellular matrix geometry for spreading and adhesion.

Cell adhesion, spreading and migration require the dynamic formation and dispersal of contacts with the extracellular matrix (ECM). In vivo, the number, availability and distribution of ECM binding sites dictate the shape of a cell and determine its mobility. To analyse the geometrical limits of ECM binding sites required for cell attachment and spreading, we used microcontact printing to produce regular patterns of ECM protein dots of defined size separated by nonadhesive regions. Cells cultured on these substrata adhere to and spread on ECM regions as small as 0.1 microm2, when spacing between dots is less than 5 microm. Spacing of 5-25 microm induces a cell to adapt its shape to the ECM pattern. The ability to spread and migrate on dots > or =1 microm2 ceases when the dot separation is > or =30 microm. The extent of cell spreading is directly correlated to the total substratum coverage with ECM-proteins, but irrespective of the geometrical pattern. An optimal spreading extent is reached at a surface coating above 15%. Knowledge of these geometrical limits is essential for an understanding of cell adhesion and migration, and for the design of artificial surfaces that optimally interact with cells in a living tissue.

3T3 Cells↗

PubMed2003

Optimized staircase profiles for diffractive optical devices made from absorbing materials.

We report on the optimization of staircase grating profiles for the case of absorbing grating materials. Using a simple numerical algorithm, we determined the grating parameters, maximizing the first-order diffraction efficiency for different numbers of staircase steps. The results show that there is a significant difference between the staircase profiles for nonnegligible and negligible absorption. The obtained solutions are of importance for diffractive optics in the soft-x-ray and extreme-ultraviolet ranges.

Journal Article↗

PubMed2003

Diamond planar refractive lenses for third- and fourth-generation X-ray sources.

The fabrication and testing of planar refractive hard X-ray lenses made from bulk CVD diamond substrates is reported. The lens structures were generated by electron-beam lithography and transferred by reactive-ion etching into the diamond. Various lens designs were fabricated and tested at 12.4 and 17.5 keV photon energy. Efficiencies of up to 71% and gains of up to 26 were achieved. A line focus of 3.2 micro m (FWHM) was measured. These lenses should be able to withstand the extreme flux densities expected at the planned fourth-generation X-ray sources.

Journal Article↗