Biomedical subjects

Christian Mulder

Publications and source records attributed to Christian Mulder.

7 recordsLinked to original sources

PubMed2006

Can transgenic maize affect soil microbial communities?

The aim of the experiment was to determine if temporal variations of belowground activity reflect the influence of the Cry1Ab protein from transgenic maize on soil bacteria and, hence, on a regulatory change of the microbial community (ability to metabolize sources belonging to different chemical guilds) and/or a change in numerical abundance of their cells. Litter placement is known for its strong influence on the soil decomposer communities. The effects of the addition of crop residues on respiration and catabolic activities of the bacterial community were examined in microcosm experiments. Four cultivars of Zea mays L. of two different isolines (each one including the conventional crop and its Bacillus thuringiensis cultivar) and one control of bulk soil were included in the experimental design. The growth models suggest a dichotomy between soils amended with either conventional or transgenic maize residues. The Cry1Ab protein appeared to influence the composition of the microbial community. The highly enhanced soil respiration observed during the first 72 h after the addition of Bt-maize residues can be interpreted as being related to the presence of the transgenic crop residues. This result was confirmed by agar plate counting, as the averages of the colony-forming units of soils in conventional treatments were about one-third of those treated with transgenic straw. Furthermore, the addition of Bt-maize appeared to induce increased microbial consumption of carbohydrates in BIOLOG EcoPlates. Three weeks after the addition of maize residues to the soils, no differences between the consumption rate of specific chemical guilds by bacteria in soils amended with transgenic maize and bacteria in soils amended with conventional maize were detectable. Reaped crop residues, comparable to post-harvest maize straw (a common practice in current agriculture), rapidly influence the soil bacterial cells at a functional level. Overall, these data support the existence of short Bt-induced ecological shifts in the microbial communities of croplands' soils.

Carbon↗

PubMed2006

Driving forces from soil invertebrates to ecosystem functioning: the allometric perspective.

The European soil policy is being focussed towards a more conscious and sustainable use of the soil, taking into account ecological, economical and societal dimensions. Living soil organisms are reliable bioindicators, as they provide the best reflection of the soil system, ecological services and ecosystem functioning therein. These most complex (bio)physical systems indicate, among others, the energy flow. Such processes can be described by rather simple power law relationships. In fact, the average body mass (dry weight) can be seen as an inherent species property, while population density is a much more flexible parameter reflecting ecosystem state. In this study, I review the interactions between these items in relation to feedbacks and conjectured relationships which can be seen as ecological networks. From this novel perspective, allometry can be used as an integrated measure for the anthropogenic influence on landscapes and related food webs. Allometry is, therefore, a perfect surrogate for land use intensity in modelling of field effects for restoration ecology and conservation biology. Robust correlations will be addressed between the density dependence of invertebrates and the ability of soil systems themselves to recover after disturbance. Quantitative indicators of soil community composition and related ecological services are proposed and their application for ecological risk assessment is illustrated.

Animals↗

PubMed2005

Nonparasitic Nematoda provide evidence for a linear response of functionally important soil biota to increasing livestock density.

Soil acidity, nutrient availability and livestock density have a major influence on the belowground ecological community. As fast decomposition rates are due mostly to bacterial-based pathways and slower decomposition rates mostly to fungal-based pathways, it is helpful to condense empirical information in the so-called Nematode Channel Ratio (NCR). The NCR is shown to be a good indicator of efficiency in soil decomposition processes. We argue that in intensive agroecosystems, other fungivore members of the decomposer food web may outcompete the hyphal-feeding nematodes. We demonstrate how the NCR can be used to set ecological standards for sustainable use of the soil in agroecosystems. To summarize the interactions between the microbial resources and the decomposer nematofauna according to increasing land management, we propose the use of the fifth percentile as proxy for a sustainable environmental quality of grasslands on sandy soils, and the NCR mean as the upper threshold for low-stocked farms.

Animals↗

PubMed2005

Legislation and ecological quality assessment of soil: implementation of ecological indication systems in Europe.

Nationally and internationally there are an increasing number of legal initiatives to protect the ecological processes in soil. This article describes the legal situation concerning the protection of natural functions of soil in Europe (mainly The Netherlands (since 1987) and Germany (since 1998)). Examples of processes to be protected comprise the element cycles, the degradation of pollutants, and the conversion of organic matter. These processes are performed by organisms, and therefore, there is a growing consensus that protection of soil biodiversity is necessary for the survival of humans and the above-ground ecosystems. It is discussed that protection of soil ecosystems needs requires definitions and aims for the ecological quality of soil (as already provided for aquatic ecosystems). Taking into account that most soils are privately owned, legal initiatives are necessary to clarify responsibilities and to direct research toward the implementation of reproducible and standardized methods to determine the ecological quality of soil.

Conservation of Natural Resources↗

PubMed2005

Considerations for the use of soil ecological classification and assessment concepts in soil protection.

In comparison with other environmental compartments, the protection of soil was identified relatively late as an important issue. Even later, policy makers and the general public considered the ecological functions of the soil. However, recently, the central role of these functions, as well as the importance of the soil organism community, has become obvious in several countries in Europe and in the European Union itself. In this contribution, basic considerations referring to use of ecological endpoints for the classification and assessment of soils are discussed. The article begins with two goals of protection: to protect soil so that it can function and to protect soil as a habitat for organisms necessary for these functions. Afterward, the quality of soil from is discussed from a biological point of view. Finally, on the basis of experience from limnology and botany, recommendations are made for monitoring programs. In other articles of this series, details of the entire approach to ecological classification and assessment are outlined.

Animals↗

PubMed2005

Ecological classification and assessment concepts in soil protection.

Evaluation of ecological soil quality requires the integration of physical, chemical, and biological variables. From a biological point of view, the maintenance of biodiversity and the assurance of life support functions are the main goals of protection to secure sustainable land use. To reach these goals, biological concepts for the classification and assessment of soils were developed in various countries, most of them based on similar concepts in limnology and aquatic ecotoxicology, which are already routinely applied. In this article, an overview of existing concepts, as well as a detailed description of the two most promising ones (BISQ from The Netherlands and BBSK from Germany), is given, followed by a discussion of the properties essential to an ideal biological concept for soil protection. Finally, recommendations for further research and a basic outline of a monitoring program ready for immediate use are provided.

Animals↗

PubMed2005

The use of nematodes in ecological soil classification and assessment concepts.

Although there has been extensive applied agricultural research (research on plant-parasitic species has a long tradition), insufficient taxonomical knowledge, especially of free-living nematodes, is a serious problem concerning the use of nematodes in soil classification and assessment. However, due to their essential and various roles in ecosystem functioning and their high diversity and abundance, interest in using these organisms for the assessment of soil quality is increasing. In particular in The Netherlands, but also in other countries (e.g., Germany, United Kingdom), progress in taxonomy is being achieved and evaluation strategies are being elaborated. While examples exist for the successful use of nematodes as part of a community approach comprising several organism groups, much work concerning the establishment of an adequate reference database remains to be done. This article is a general overview of the suitability and application of soil nematodes in soil assessments.

Animals↗