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Christian von Buchwald

Publications and source records attributed to Christian von Buchwald.

10 recordsLinked to original sources
PubMed2006

Human papillomavirus and pterygium. Is the virus a risk factor?

BACKGROUND: Pterygium is a disease of unknown origin and pathogenesis that might be vision threatening. It is characterised by a wing-like conjunctival overgrowth of the cornea. Several studies have investigated human papillomavirus (HPV) as a risk factor for the development of pterygia, but the results are inconclusive. AIM: To investigate a large sample of pterygia for the presence of HPV in order to clarify the putative association between pterygia and HPV. METHODS: 100 specimens of pterygium from Danish patients and 20 normal conjunctival biopsy specimens were investigated for the presence of HPV with PCR technique using beta-globin primers to access the quality of the extracted DNA and the HPV primers MY09/11 and GP5+/6+. HPV-positive specimens underwent subsequent HPV typing with type-specific HPV primers and further investigation with DNA in situ hybridisation (ISH). RESULTS: 90 of 100 investigated pterygia proved suitable for HPV analysis by PCR. As beta-globin could not be amplified, 10 specimens were excluded from the study. 4 of 90 pterygia harboured HPV. HPV type 6 was identified in all four HPV-positive pterygia. The 20 normal conjunctival biopsy specimens were beta-globin positive and HPV negative. All four pterygia that were HPV type 6 positive were DNA ISH negative. CONCLUSIONS: The low presence of HPV DNA in pterygia does not support the hypothesis that HPV is involved in the development of pterygia in Denmark.

Adult↗
PubMed2006

Human papillomavirus in normal conjunctival tissue and in conjunctival papilloma: types and frequencies in a large series.

AIM: To examine conjunctival papilloma and normal conjunctival tissue for the presence of human papillomavirus (HPV). METHODS: Archival paraffin wax-embedded tissue from 165 conjunctival papillomas and from 20 histological normal conjunctival biopsy specimens was analysed for the presence of HPV by PCR. Specimens considered HPV positive using consensus primers, but with a negative or uncertain PCR result using type-specific HPV probes, were analysed with DNA sequencing. RESULTS: HPV was present in 86 of 106 (81%) beta-globin-positive papillomas. HPV type 6 was positive in 80 cases, HPV type 11 was identified in 5 cases and HPV type 45 was present in a single papilloma. All the 20 normal conjunctival biopsy specimens were beta-globin positive and HPV negative. CONCLUSION: There is a strong association between HPV and conjunctival papilloma. The study presents the largest material of conjunctival papilloma investigated for HPV and the first investigation of HPV in normal conjunctival tissue. HPV types 6 and 11 are the most common HPV types in conjunctival papilloma. This also is the first report of HPV type 45 in conjunctival papilloma.

Adolescent↗
PubMed2006

The Danish national guidelines for treatment of oral squamous cell carcinoma.

The treatment strategy for oral squamous cell carcinoma in Denmark has traditionally varied between the different head and neck oncology centres. A study group within the Danish Society for Head and Neck Oncology (DSHHO) was formed with the aim of optimising and standardising the treatment strategy. The approach was to use single modality treatment for stage I, stage II and some stage III and combined modality treatment for stage III and IV. Surgery was the preferred treatment when it was considered possible to perform a radical excision of the tumour and possible lymph node metastases with acceptable aesthetic and functional outcome. The implementation of a recognised national guideline facilitates prospective studies on a large well-characterised cohort. This increases the possibility of obtaining valid data on parameters such as morbidity, loco-regional control and survival. In addition the establishment of a reference program facilitates national monitoring of the treatment using defined indicators and standards.

Carcinoma, Squamous Cell↗
PubMed2003

[An extensive case of maxillary pneumosinus dilatans].

We present a 40-year-old male with an aggressive case of maxillary pneumosinus dilatans. Our examination including rhinoscopy showed that the nasal wall including the middle turbinate was displaced towards the nasal septum obliterating the middle meatus. A reopening of the affected maxillary sinus was performed endoscopically with success. At the follow-up examination and exposure to high altitude simulation in an underpressure chamber 15 months postoperatively the patient had no symptoms and there were no signs of exopthalmus or facial swelling.

Adult↗
PubMed2002

[Sentinel node biopsy in head and neck cancer. Is there an indication for use of this new surgical technique in the treatment of head and neck cancer?].

Management of the clinically N0 neck in head and neck squamous cell carcinoma remains controversial. Since lymph node metastasis reduces the five-year survival by up till 50%, the need for an accurate diagnostic procedure is necessary. The sentinel node, being the initial lymph node to which the primary tumour drains, is believed to be invaded by early metastasis. Identification of the sentinel node by means of a dye or isotope or a combination, and surgical removal followed by histological examination verifies the involvement of the regional glands. At present few and limited materials on head and neck cancers exist, primarily on oral and oropharyngeal cancers. The techniques used are various and not without difficulties. To evaluate the feasibility of the sentinel node biopsy in head and neck cancer, it is proposed to perform a nationwide cohort study that comprises all minor oral and oropharyngeal cancers (T1, T2, N0).

Carcinoma, Squamous Cell↗
PubMed2002

[Oral focal epithelial hyperplasia. Any risk of confusion with oral condylomas?].

INTRODUCTION: Focal epithelial hyperplasia (FEH) is a disease of the oral mucosa caused by human papilloma virus (HPV) types 13 and 32. It is not very common in Denmark and thus not well known to most physicians. It is rarely seen in Caucasians, but in other parts of the world, especially in the southeast Mediterranean countries, among the native Indians of South and Central America, and in Eskimos the disease is rather common. FEH presents with exophytic lesions of the oral mucosa and may be rather easily confused with papillomas or condylomas, and therefore perhaps give rise to the suspicion of sexual abuse in children. We describe easy ways of making an accurate diagnosis of FEH and other HPV-related diseases. MATERIAL AND METHODS: We present five patients who were diagnosed with FEH by in situ hybridisation on biopsies or by conducting PCR with HPV type-specific consensus primers followed by analysis of the restriction fragment length polymorphism. RESULTS: FEH could be diagnosed in all five patients by the two procedures described. In three cases, both methods were applied and gave identical results. Three patients were infected with HPV type 32 and two patients with HPV type 13. Co-infection with more than one HPV type was not found. DISCUSSION: Many physicians rarely encounter FEH, and will thus have difficulty in reaching an accurate diagnosis. In doubtful cases, oral papillomatous or exophytic lesions should be subjected to HPV typing. When suspicion of oral condylomas is raised, particularly in children, accurate diagnosis is necessary to rule out suspected sexual abuse. Viral DNA for HPV typing can be easily obtained, simply by scraping the lesion.

Adolescent↗
PubMedDate not supplied

Sentinel node biopsy: the technique and the feasibility in head and neck cancer.

Management of the clinically N(0) neck in head and neck squamous cell carcinoma is still under debate. Tumour spread to the neck is the most important prognostic factor in head and neck cancer patients. The sentinel node technique comprises the identification of the sentinel node by means of dye or isotope or a combination, and surgical removal followed by histological examination. We have reviewed the preliminary reports indicating that sentinel node identification is technically feasible in head and neck cancer surgery, i.e. in solitary and unilaterally oral and pharyngeal cancer stages T1 and T2 with clinical N(0). However, the existing reports enrole observational studies, thus randomised trials should be considered to gain maximum valid data to prove that sentinel node biopsy has an effect on parameters such as loco-regional control and survival.

Carcinoma, Squamous Cell↗
PubMedDate not supplied

Unexpected expansive paranasal sinus mucormycosis.

Mucormycosis infection of the paranasal sinuses is a very rare but serious condition. Patients are usually immunocompromised by systemic disease. We report an unexpected case of expansive paranasal sinus mucormycosis in an immunocompetent European treated successfully with surgical debridement and intravenous amphotericin B. The pathogenesis, diagnosis and treatment of paranasal sinus mucormycosis is discussed.

Anti-Bacterial Agents↗