Biomedical subjects

Christin Tse

Publications and source records attributed to Christin Tse.

3 recordsLinked to original sources

PubMed2005

Predicting protein druggability.

The ability to predict whether a particular protein can bind with high affinity and specificity to small, drug-like compounds based solely on its 3D structure has been a longstanding goal of structural biologists and computational scientists. The promise is that an accurate prediction of protein druggability can capitalize on the huge investments already made in structural genomics initiatives by identifying highly druggable proteins and using this information in target identification and validation campaigns. Here we discuss the potential utility of tools that characterize protein targets and describe strategies for the optimal integration of protein druggability data with bioinformatic approaches to target selection.

Binding Sites↗

PubMed2002

Semaphorin 3F gene from human 3p21.3 suppresses tumor formation in nude mice.

Loss of heterozygosity on human chromosome 3p21.3 is a frequent occurrence in many tumor types. In a previous study, our laboratory demonstrated that an 80-kb P1 clone from chromosome 3 suppresses the tumorigenicity of the mouse fibrosarcoma cell line A9. Two cDNAs corresponding to genes encoded on this P1 clone, semaphorin 3F (SEMA3F) and N23, were tested for their effects on in vitro and in vivo growth characteristics after transfection into mouse A9 cells. Transfection of SEMA3F cDNA resulted in complete loss of tumorigenicity in nude mice, whereas transfection of N23 had no effect. Moreover, SEMA3F also functioned to block apoptosis of transfected A9 cells treated with Taxol or Adriamycin. The human ovarian adenocarcinoma cell line HEY showed a similar result as A9 cells, but the small cell lung cancer line GLC45 was unaffected by expression of SEMA3F.

Adenocarcinoma↗

PubMed2002

Human Semaphorin 3B (SEMA3B) located at chromosome 3p21.3 suppresses tumor formation in an adenocarcinoma cell line.

The short arm of chromosome 3 has been shown to exhibit high loss of heterozygosity in several types of cancer including ovarian, kidney, lung, and testicular cancers. In particular, overlapping homozygous deletions in lung cancers have been identified in region 3p21.3. Semaphorin 3B, a gene that resides within this region, has been proposed to be involved in tumorigenesis. To address this hypothesis, we have examined the effects of semaphorin 3B on HEY cells, an ovarian cancer cell line. HEY cells expressing semaphorin 3B exhibited a diminished tumorigenicity in BALB/c nu/nu mice. Semaphorin 3B also severely reduced the anchorage independence of HEY cells. These results demonstrate a role for semaphorin 3B in tumor suppression.

Adenocarcinoma↗