Biomedical subjects

Chuanyu Liang

Publications and source records attributed to Chuanyu Liang.

At least 19 recordsLinked to original sources

PubMed2006

[Anesthesia selection in hypophysectomy by endoscopic transnasal sphenoidal approach].

OBJECTIVE: To explore the anesthesia selection in resection of pituitary neoplasms by endoscopic transnasal sphenoidal approach. METHOD: Fifty-six cases with pituitary tumor underwent transnasal sphenoidal endoscopic surgery . Among the 56 cases, 8 cases accepted general anesthesia and 48 cases accepted local anesthesia and sedation. RESULT: All patients had no complains of surgery. 48 cases who accepted local anesthesia and sedation were completed surgery successfully, without graining and struggling during the operation. CONCLUSION: Local anesthesia and sedation is a simple, safe and good selection for resection of pituitary neoplasms by endoscopic transnasal sphenoidal approach.

Adolescent↗

PubMed2006

[Endoscopic repair of cerebrospinal fluid rhinorrhea].

OBJECTIVE: To investigate the best treatment of cerebrospinal fluid rhinorrhea. METHOD: A retrospective study of 45 consecutive patients who presented with CSF leak,was conducted. Forty-two patients were treated with transnasal endoscopic approach, three with double approach transnasal and extranasal. The fascia lata, the myoplasm, the free middle turbinate graft and the free inferior turbinate graft were used for fistula repairing. Four repair techniques as onlay technique, inlay technique, underlay technique and obliteration technique were used. RESULT: Postoperative follow-up lasted from 5 to 84 months. The overall success rate after the first operation was 97.8%. Three patients got meningitis,one patient got local infection in frontal sinus, but all of them were cured. CONCLUSION: In our study, The outcome was very good in endoscopic repair of a small fistula in anterior cerebrospinal, a fistula in dorsum delta and a fistula in slope with a free graft of autogenous. But it needed a perfect choose in operation approach, repair technique and graft material.

Adolescent↗

PubMed2006

[Cloning human heat shock protein 90beta-cDNA and constructing its eukaryon vector].

The purpose of this study was to clone human HSP90beta cDNA and construct its eukaryote expression vector . The total RNA was isolated by TRIzol Reagent (Invitrogen) from human NPC and its cDNA was gained by RT-PCR. The purified RT-PCR products and PGEM-T Easy Vector were ligated and transformed into XL1-blue E. coli bacteria. The white clones were selected and the plasmid was purified, which was further identified by double enzyme digestion and sequenced. PGEM-hHSP90beta and pcDNA3.1(+) DNA were digested by AflII and Xbal respectively. After purification, the two fragments obtained were ligased by using T4 DNA ligase (Fermentas). This recombinant DNA was then transformed into E. coli Competent Cells XL1-blue and positive clones were selected on the LB agarose plate containing Ampr (100 microg/ml). Single clones were identified by double digestion with AflII and Xbal, and two fragments with the size 5.4 kb and 2.1 kb were produced as expected. The hHSP90beta gene was successfully inserted into the eukaryote expression vector pcDNA3.1(+) by the recombination technique in vitro.

Cloning, Molecular↗

PubMed2005

[Study on chondrogenesis of rabbit bone marrow-derived mesenchymal stem cells by centrifuge-tube culture in vitro].

OBJECTIVE: To investigate the feasibility of constructing tissue engineered cartilage by inducing bone marrow-derived mesenchymal stem cells (MSCs) in centrifuge-tube culture. METHOD: By Percoll density gradient centrifuge and monolaryer culture in vitro MSCs were isolated from rabbit bone marrow and were purified. Some MSCs were seeded into centrifuge tube culture and allowed to form three-dimensional aggregates in a chemically defined medium mainly including transforming growth factor- b1 (TGF-beta1, 10 ng/ml), dexamethasone (10-7), ascorbic acid (50 microg/ml), 1% insulin, 1% selenium and 1% transferring. At day 7, 14, 21, aggregates were take out and specific markers of differentiation of cartilage were detected by the appearance of toluiding blue merachromasia and the immunohistochemistry detection of type II collagen and in situ hybridization detection of collagen type II mRNA. RESULT: MSCs seeded into centrifuge tube culture became analogous cartilage tissue after 7-21 days, their specific markers of chondrogeneses were positive in the appearance of toluiding blue merachromasia and the immunohistochemistry detection of type II collagen and in situ hybridization detection of collagen type II mRNA. CONCLUSION: MSCs have the capacity to undergo chondrogenic differentiation.

Animals↗

PubMed2005

[Repair of thyroid cartilage defects with chondrocyte-allogenous acellular cartilaginous matrix composite in rabbits].

OBJECTIVE: To investigate the feasibility of repairing thyroid cartilage defects by implantation of chondrocyte-allogenous acellular cartilaginous matrix (chondrocyte-ACM) composite in rabbits. METHODS: The thyroid chondrocytes were isolated and co-cultured in vitro with allogenous acellular cartilaginous matrix (ACM) to form the chondrocyte-ACM composite. The composite was analyzed histologically and was used to repair defects of thyroid cartilage. Eighteen New Zealand adult rabbits were made the defect models of thyroid cartilage at the two sides and divided into three groups. The defects were repaired with chondrocyte-ACM composite in the experimental group (n= 6), with simple ACM in the ACM group (n=6) and without any material in the control group (n=6). The animals were sacrificed at 8 weeks after operation. The specimens were evaluated histologically. RESULTS: In vitro, the growth of chondrocytes was observed on the surface of allogenous acellular cartilaginous matrix and no chondrocytes grew inside the matrix. The defect filled with muscle and connective tissues in control group; the lymphocyte infiltration was observed in the matrix and no new cartilage formation occurred at 8 weeks after operation in simple ACM group and experimental group. So the defect repair of rabbits thyroid cartilage failed. CONCLUSION: The allogenous acellular cartilaginous matrix failed to serve as a scaffold for chondrocytes both in vitro and in vivo. The allogenous acellular cartilaginous matrix should be improved.

Animals↗

PubMed2005

[The treatment of sinusitis in patients with nasopharyngeal carcinoma after radiotherapy and the relative factors].

OBJECTIVE: To explore the surgery way on sinusitis with nasopharyngeal carcinoma (NPC) caused by radiotherapy and the relative factors. METHOD: Forty-five cases of NPC patients with sinusitis caused by radiotherapy were treated by endoscopic sinus surgery in our department from 1998 to 2003. There were 24 males and 21 females, ranging in age from 34 to 45 years old. The clinical data of the patients were analysed retrospectively. RESULT: All the patients were followed up for a period of 1 to 4 years after operation. 22 cases (48.9%) were cured, 18 cases (40%) were improved, but the other 5 cases (11.1%) were no effective. No serious complications and tumor recurrence occurred. CONCLUSION: The incidence of sinusitis in patients with NPC after radiotherapy is very high which interferes the effect of radiotherapy on NPC. Endoscopic sinus surgery is an effective method to open sinus ostium. It is feasible and favorable.

Adult↗

PubMed2005

[Clinical analysis of infectious mononucleosis misdiagnosised as tonsillitis].

OBJECTIVE: Discussing the clinical features of infectious mononucleosis misdiagnosised as tonsillitis. METHOD: We used the methods of variant lymphocyte count, heterophil agglutination test and EBVCA-IgA detection to diagnose 6 cases of infectious mononucleosis misdiagnosised as tonsillitis. RESULT: Six cases of infectious mononucleosis misdiagnosised as tonsillitis are cured without upper airway obstruction. CONCLUSION: We must pay attention to the variant lymphocyte count when tonsillitis is diagnosed. Some cases of infectious mononucleosis in convalescence or without hepatic functional lesion and renal functional lesion are easily misdiagnosised and missed.

Adult↗

PubMed2005

[Study on the expression of HSP70 and HSP90beta in nasopharyngeal carcinoma and the clinical significance].

OBJECTIVE: To study the expression of HSP70 and HSP90beta and its clinical significance in human nasopharyngeal carcinoma. METHOD: Immunohistochemical method SP was used to detect the expression of HSP70 and HSP90beta in 50 cases and its clinical significance were studied. RESULT: The positive rate of HSP70 and HSP90beta were 72% and 56% respectively. Analysis of patients, survival demonstrated that the prognosis of NPC with HSP70 (-) and HSP90beta (-) expression were significantly better than the others. CONCLUSION: The overexpression of HSP70 and HSP90beta were probably concerned with the occurrence, development and prognosis of nasopharyngeal carcinoma.

Adolescent↗

PubMed2005

[A randomized controlled trail on vasodilators and steroids for sudden sensorineural hearing loss].

OBJECTIVE: To assess the therapeutic efficiency of dextran and steroids for sudden sensorineural hearing loss (SSHL) and to investigate the correlation factors of the prognosis. METHOD: In this multi-center clinical trial, 100 patients who were diagnosed of SSHL were randomly divided into dextran treatment group and steroid treatment group. Eleven correlation factors of the prognosis and therapeutic efficiency of SSHL were analyzed through a logistic regression model. RESULT: Four factors entered the logistic regression model. They were the mean value of high frequency of hearing threshold, vertigo, pattern of hearing loss and treatment programme. CONCLUSION: Steroid was more effective in the treatment of SSHL compared with dextran.

Adolescent↗

PubMed2005

[Surgical treatment of peripheral facial nerve paralysis: analysis of 51 cases].

OBJECTIVE: To investigate the surgical treatment of peripheral facial nerve paralysis as well as the factors which affect the operation effect. METHOD: Fifty-one cases of peripheral facial nerve paracusis were analyzed retrospectively. RESULT: Forty-two cases were followed up. Among them, twenty-two cases who received facial nerve decompression reached House I-II degree (22/36), 2 cases facial nerve anastomosis reached House I-II degree (2/4), and the function recovery of 2 cases who received facial nerve grafting > III degree both. CONCLUSION: Intensive and timely surgery is an efficient way in treating of peripheral facial nerve paralysis.

Adolescent↗

PubMed2005

[Pathological and ultrastructural observation on the epithelia of nasal cavity and nasal sinuses in chronic sinusitis and nasal polyps after endoscopic sinus surgery].

OBJECTIVE: To research the restoration characteristics of epithelia in nasal cavity and nasal sinuses with chronic sinusitis and nasal polyps after endoscopic sinus surgery,and to provide basis for improving the treatment after operation. METHOD: Twenty patients with chronic sinusitis and nasal polyps after endoscopic sinus surgery were observed. The "Vesicle tissues" situated ostiomeatal complex (OMC) were observed with light and transmission electron microscopy after operation of 7-14 days. RESULT: (1) The "Vesicle tissues" were off white or buff, some contained liquid, and some were parenchyma under the endoscopy. (2) The epithelia of "Vesicle tissues" were pseudostratified ciliated columnar epithelium (16/20), and some were stratified squamous epithelium (2/20), some were squamous metaplasia in pseudostratified ciliated columnar epithelium (2/20). Lamina propria were edema. Inflammation cells infiltrated epithelial lining and subepithelial lining. We found mucous gland, mixed gland and glandular duct under subepithelial lining,and small vascular ectasia under light microscopy. (3) Transmission electron microscope: epithelia were pseudostratified ciliated columnar epithelium, cilia were in good order, microvilli located between cilia. The cross section of majority cilia were 9 + 2 form microtubule, but in some cilia we could not find microtubule and could only find one central microtubule. Golgi complex were up growth, and endoplasmic reticulum expand, and multi-mitochondrion were coagulation degeneration in epithelial cells. Some mitochondrion were 1-2 degree swelling and the lysosome increased. Inflammation cells infiltrated epithelial lining. Majority were neutrophil and lymphocyte, but we also found eosinophil and plasmacyte. CONCLUSION: "Vesicle tissues" is the manifestation of active epithelia regeneration in nasal cavity and nasal sinuses after endoscopic sinus surgery, it's the earlier period of imbalance regeneration of the epithelization in postoperative cavity. "Vesicle tissues" is similar to the nasal polyp histopathology, and it is phenomena of limited pathological rebuilding in earlier period. Cleaning "Vesicle tissues" can effectively control the nasal polyps relapse and impel the postoperative cavity to epithelization.

Adult↗

PubMed2005

[In vitro study of the directed inducing cartilage cells from rat bone mesenchymal stem cells on scaffolds].

This study was conducted to assess the cell proliferation and cell cycle of the directed inducing cartilage cells from rat bone mesenchymal stem cells on collagen sponge and compare the results with those of the plane inducing cartilage cells. The MSCs were isolated from rat bone marrow by density gradient centrifuge and adhesive property, and then were induced into chondrogenic differentiation by adding transforming growth factor-beta1 (TGF-beta1), dexamethasone, transferring and insulin on collage sponge and plane condition. Immunohistochemistry was applied to detect the expression of collagen type II on the scaffolds and plane conditions. The cell proliferation and cell cycle on different conditions were detected with MTT automated colormetric microassay and flow cytometry. The differentiation of MSCs toward chondrogenic phenotype was verified by the positive result of collagen type II on the scaffolds and plane condition. The cell viability in scaffolds was better than that in plane conditions. The S phase and G2 phase were 30 percent in whole cell cycle on the scaffold, which was different from that of the control groups. The method of using the Using directed inducing cartilage from bone MSCs combined with collage sponge to construct tissue-engineered cartilage is feasible; it is better than the method of plane directed inducing.

Animals↗

PubMed2005

[A clinical observation on the effect of irrigating solution of Shuang Huang Lian on ESS for chronic sinusitis].

OBJECTIVE: To investigate the effect of irrigating solution of Shuang Huang Lian on ESS for chronic sinusitis. METHOD: In 201 cases with chronic sinusitis (type II) treated by ESS From August 2001 to August 2004, the Shuang Huang Lian Liquid and 0.9% N.S. were respectively used in 101 cases and 100 cases to irrigate the operated sinus cavity, then a comparison was made between the two groups about the irrigating solution with above six months followed up. RESULT: The total effective ratio in group Shuang Huang Lian was significantly higher than that in group of 0.9% N.S. (P < 0.05). CONCLUSION: Irrigating solution of Shuang Huang Lian used after operation can improve the therapeutic effect of ESS for chronic sinusitis.

Adolescent↗

PubMed2004

[Clinical analysis on 18 cases of primary nasal cavity and sinuses T/NK lymphomas].

OBJECTIVE: To investigate the clinical characteristics and treatment effect of primary sinonasal T/NK lymphomas. METHOD: Eighteen cases of primary sinonasal T/NK lymphomas were reviewed retrospectively. The clinical behavior, clinical diagnostic characteristics and prognosis were studied. RESULT: The clinical symptoms of these patients included nasal obstruction, epistaxis, nasal discharge, intumescence, ulcer and fever so on. Clinical diagnosis was easy to be misdiagnosed. The prognosis of patients that higher stage and higher malignant were bad. CONCLUSION: Sinonasal T/NK lymphomas are a distinct clinicopathological category and the clinical symptoms are nonspecificness. We should pay attention to distinction and avoiding misdiagnosis. The prognosis is relative to pathological category and clinical stage.

Adolescent↗

PubMed2004

[Expression of VEGF-C and VEGFR-3 in human laryngeal squamous cell carcinomas and cervical metastatic lymph node tissues].

OBJECTIVE: To study the gene expression of vascular endothelial growth factor C (VEGF-C) and vascular endothelial growth factor receptor-3 (VEGFR-3) in laryngeal squamous carcinoma and their relation with clinic pathologic features. METHOD: VEGF-C and VEGFR-3 gene expression in 30 cases of normal laryngeal mucosa tissue (NLM), primary laryngeal carcinoma (PLC) and cervical lymph node (CLN) were examined by reverse transcription polymerase chain reaction (RT-PCR). RESULT: The gene expression of VEGF-C and VEGFR-3 are different among normal laryngeal mucosa tissue, primary laryngeal carcinoma and cervical lymph node in the same patient. In primary laryngeal carcinoma, expression of VEGF-C, VEGFR-3 were significantly higher in lymph node positive group than in lymph node negative group and associated with histological grade of differentiation; The gene expression of VEGF-C, VEGFR-3 were not associated with age, sex, site, T stage. CONCLUSION: VEGF-C, VEGFR-3 have prognostic significance in laryngeal carcinoma.

Aged↗

PubMed2004

[Diagnosis and following-up and analysis of pathogenesis for congenital hearing loss].

OBJECTIVE: To investigate the incidence and the relevant high-risk factors of congenital hearing loss for infants from well-baby nursery and newborn intensive care unit. To explore the scientific and feasible mode of early diagnosis, following-up and early intervention. METHODS: Two-stage universal newborn hearing screening ( UNHS ) were performed by using transient otoacoustic emission ( TEOAE ). Auditory brainstem responses ( ABRs) and 40 Hz auditory event related potentials (40 Hz-AERPs) was used as diagnostic tests for those failed re-screening at three-month old. Two kinds of infants who had abnormal ABRs or high-risk factors of hearing loss received following-up and routine audiological evaluation from 6 months to 3 years of age. Each infant with congenital hearing loss received personalized intervention. RESULTS: The incidence of congenital hearing loss among infants who received UNHS was 5.73 per thousand. The incidence of congenital hearing loss was 20.02 per thousand in NICU and 3.67 in WBN. CONCLUSIONS: Whether passed the UNHS or not , infants from NICU should receive ABR and 40 HzAERP tests. The following-up population includes infants with abnormal ABRs or high-risk factors of hearing loss. Following-up and early intervention should be personalized.

Early Diagnosis↗

PubMed2003

[Proteomics analysis and biochemical activity detection on facial nerve regeneration fluid].

OBJECTIVE: To explore the protein components and the biological neutrional activity upon motor neuron, so that take a general, basic study on the nerve regeneration microenvironment which be important to facial nerve regeneration. METHOD: The animal model of rabbit facial regeneration microenvironment is established. Advanced technology of two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) was used to study on the structural proteins in the regeneration fluid, as well as the motor neuron culture was used to test the biological activity in it. RESULT: 850 +/- 78 protein spots were detected in the regeneration fluid. The body area, neurite length and OD value of experimental group are higher than that of control group. CONCLUSION: We got the protein image of facial nerve regeneration fluid by 2-D PAGE, and proved that many kinds and number of proteins in the regeneration fluid have neutrional activity on motor neuron.

Animals↗

PubMed2003

[An analytical study and report on 23 cases of neurolemmoma in the head and neck].

OBJECTIVE: To improve the understanding of the diagnosis and treatment of neurolemmoma in the head and neck by exploring its clinical features. METHOD: Among all cases treated surgically, 15 cases of neurolemmoma in the neck were by the approach of lateral pharyngotomy, one case of neurolemmoma in the nasal cavity by lateral rhinotomy, three cases by mouth. Two cases of facial neurolemmoma were resected by the combined approach (mastoid process, middle cranial fossa and posterior cranial fossa) and radical mastoidectomy respectively. One case of neurolemmoma in the pyriform sinus was completely removed by the forceps under esophagoscope. One case of neurolemmoma in the trachea was removed by tracheotomy. RESULT: Twenty cases were followed up from 3 months to 7 years and 19 cases did not recur. One case of neurolemmoma in the nasal cavity and sinus recured 3 years after operation. One case developed Horner's syndrome postoperatively and disappeared after two months. One case developed hoarseness. CONCLUSION: It is difficult to diagnose neurolemmoma before operation. Diagnosis of the disease is based on pathological examination. Resection of the tumor should be performed as soon as possible under careful protection of the function of the nerve in order to avoid severe complications.

Adolescent↗