Biomedical subjects

Chun-Hong Wang

Publications and source records attributed to Chun-Hong Wang.

5 recordsLinked to original sources

PubMed2006

Protein-imprinted polymer with immobilized assistant recognition polymer chains.

Here we introduce a new method for preparing a protein-imprinted polymer with immobilized assistant recognition polymer chains as an additional element of monomer to create effective recognition sites. In this work the bovine serum albumin was used as template and the template protein was selectively assembled with immobilized assistant recognition polymer chains from their library, numerous limited length polymer chains with randomly distributed recognition sites and immobilizing sites. These assemblies of protein and immobilized assistant recognition polymer chains would be adsorbed by the macro porous adsorbent spheres and immobilized by cross-linking polymerization. After removing the template, binding sites that were complementary to the target protein in size, shape and position of recognition groups were exposed, and their confirmation was preserved by the cross-linked structure. The synthesized imprinted polymer was used to adsorb BSA from protein mixtures, and showed a high selectivity.

Biocompatible Materials↗

PubMed2005

[Apolipoproptein E-CI-CII gene cluster and its effect on serum lipid levels].

OBJECTIVE: To investigate the allele frequencies of the apolipoprotein (apo)E-CI-CII cluster gene in Hans of Chinese, and its relation to serum lipid levels. METHODS: The study subjects consisted of healthy individuals (male: 196, female: 154; average age: 63.6 +/- 12.0 years) who were randomly selected from health screening. ApoE genotypes were identified by multi-AMRS PCR and both the ApoCI promoter polymorphisms and AvaII polymorphisms of the apoCII gene were detected by using PCR-RFLP. The serum lipid levels were determined using an automatic chemistry analyzer. RESULTS: For ApoE gene, the frequency of E3/3 was 73.4%, it represents the most common genotype from of the polymorphism. The frequencies of E2/2, E2/4 and E4/4 genotypes were all smaller than 1%. For the ApoCI locus, the frequencies of H1/ 1, H2/2 genotypes were 79.7% and 1.7%, respectively. Both the ApoE and ApoCI polymorphisms showed the lack of association with plasma levels in this population. The linkage disequilibrium between ApoE with ApoCI wasn't found, either. The allele frequencies of ApoCII gene were T1 : 70.7% and T2 : 29.3%, respectively. The TG level in subjects carrying with T1/2 (1.49 +/- 0.96 mmol/l) was significant higher than that in subjects with T1/1 (1.29 +/- 0.72 mmol/l), P < 0.05. No significant differences were found between male and female in genotype distribution and allele frequencies of the ApoE-CI-CII gene cluster. CONCLUSION: This result suggested that it might be one of the factors in explaining the lower prevalence rate of atherosclerosis in the China population.

Adult↗

PubMed2004

Sulfotransferase 1A1 (SULT1A1) polymorphism and breast cancer risk in Chinese women.

BACKGROUND: Sulfonation catalyzed by sulfotransferase enzymes plays an important role in chemical defense mechanisms against various xenobiotics but also bioactivates carcinogens. A major human sulfotransferase, SULT1A1, catalyzes the sulfation of a variety of phenolic and estrogenic compounds. A functional polymorphism of the SULT1A1 gene has been implicated in a decreased activity and thermostability when the wild-type arginine (Arg) at codon 213 is substituted by a histidine (His). METHODS: We investigated the association between the His allele and the risk breast cancer in 213 cases and 430 matched controls in Chinese women, and the interaction between His allele and endogenous estrogen and dietary mutagens exposure factors were also determined by use of logistic regression analysis. RESULTS: There was no significant difference in genotypes between the cancer patients and control populations. However, the frequency of the His allele in cases (13.6%) were significant higher than that in controls (9.5%), P = 0.03. Compared with women carrying the Arg/Arg genotype, the adjusted odds ratio (OR) of Arg/His was 1.48 (95% CI = 0.59-3.31) and His/His was 2.28 (95% CI = 0.69-9.58), P trend was 0.04. The adjusted OR of Arg/His + His/His was 2.60 (95% CI = 1.12-6.05). His allele strengthen the effect of endogenous estrogen exposure with interaction index r > 1, and weaken the effect of heterocyclic amines and polycyclic aromatic hydrocarbons derived from dietary with interaction index r > 1, both were multiplicative interaction model. CONCLUSIONS: Our findings suggest that the SULT1A1 His allele was positively associated with the risk of breast cancer in Chinese women. And there was interaction between SULT1A1 polymorphism and related exposure factors.

Adult↗

PubMed2004

[Genetic association of apoE and apoCI gene polymorphisms with coronary heart disease].

OBJECTIVE: To study the genetic association of apolipoprotein (apo) E and apoCI gene polymorphisms with coronary heart disease (CHD) in China. METHODS: apoE genotypes were identified by multiplex amplification refractory mutation system (multi-ARMS) and the apoCI promoter polymorphisms were detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) in 186 cases with CHD (age: 65.0 +/- 10.5 years) and 350 controls (age: 63.6 +/- 8.3 years). The haplotype frequencies were estimated. RESULTS: The frequencies of apoE E4/3 genotype (26.9%) and epsilon4 (14.5%) in CHD group were significantly higher than that in the control group (12.6%, 7.0%), P <0.05. The significant difference was also found for the apoCI locus and the CHD group showed higher rate of both for the H2 allele and genotypes, carrying this allele. Estimation of the haplotype frequencies indicated that the association between the apoE-CI haplotype and CHD was significantly strong. The apoE-epsilon4/apoCI-H2 was estimated to be responsible for 9.86% of CHD. CONCLUSION: When the subjects carrying both epsilon4 and H2 alleles, they would have higher risk of suffering from CHD than controls.

Adult↗

PubMed2003

[Meta-analysis for relationship between apoE gene polymorphism and coronary heart disease].

OBJECTIVE: To evaluate relationship between ApoE gene polymorphism and coronary heart disease (CHD). METHODS: Meta-analysis was applied with a random-effect model for the collected data. RESULTS: Difference in pooled frequencies, d, of apoE genotypes E3/2, E4/2, E3/3, E4/3 and E4/4 between case and control groups were 2.3%, -0.8%, -8.5%, 10.5% and 0.9%, respectively. Difference in pooled frequencies, d, of apoE alleles epsilon2, epsilon3 and epsilon4 were -1.5%, -4.2% and 5.8%, respectively, with a statistical significance between four groups. CONCLUSIONS: apoE gene polymorphism was involved in coronary heart disease. Persons with apoE E3/3 genotype or epsilon3 allele were not susceptible to CHD, but those with apoE E4/4 genotype or epsilon4 allele had higher risk suffering from CHD than others.

Apolipoproteins E↗