Biomedical subjects

Chun-hua Fu

Publications and source records attributed to Chun-hua Fu.

4 recordsLinked to original sources

PubMed2005

[Association between Bacteroides forsythus in the infected root canals and clinical symptoms of chronic apical periodontitis].

OBJECTIVE: To investigate the distribution of Bacteroides forsythus in root canals with chronic apical periodontitis and to determine its associations with clinical symptoms. METHODS: Thirty-eight tooth root canals from 31 subjects were studied with a 16S rDNA-directed polymerase chain reaction (PCR). These teeth were classified into symptomatic and asymptomatic groups according to the clinical symptoms and signs, including spontaneous pain, percussion pain, sinus tract and swelling, respectively. RESULTS: Ten of the 38 root canal samples were positive for B. forsythus. The prevalence of B. forsythus was 26.3% for 38 root canals, 45.5% for spontaneous pain group, 39.1% for percussion pain group, 29.4% for sinus tract group, 42.9% for swelling group, respectively. Significant positive associations were observed between B. forsythus in infected root canals and the spontaneous pain, percussion pain, and swelling of apical periodontitis, respectively (OR=infinity, 9, 12; P<0.05). There was no significant association between B. forsythus and sinus tract of apical periodontitis (OR=1.33). CONCLUSION: B. forsythus colonized in the infected root canals. It is the putative pathogen of apical periodontitis.

Adolescent↗

PubMed2005

[Colonization relationship between Porphyromonas gingivalis and Bacteroides forsythus in the infected root canals with chronic apical periodontitis].

PURPOSE: To detect the distribution of P. gingivalis and B. forsythus in the infected root canals from Chinese chronic apical periodontitis, and investigate the colonization relationship between them in the root canals. METHODS: P. gingivalis and B. forsythus in the root canal samples of thirty-eight teeth with chronic apical periodontitis from 31 subjects who were referred to the Sichuan University West China Dental Hospital for dental treatment were studied with a 16S rDNA-directed polymerase chain reaction method. Fisher's exact tset was used to detect B. forsythus in the infected root canals with or without P. gingivalis. OR was used to analyse the relationship between them. RESULTS: The prevalence in 38 teeth was 39.5% for P. gingivalis, and 26.3% for B. forsythus, respectively. Significant positive relationship was shown in the combination of P. gingivalis and B. forsythus (OR=12, P<0.05). CONCLUSION: Both P. gingivalis and B. forsythus colonized in the root canals with chronic apical periodontitis and there was a positive relationship between P. gingivalis and B. forsythus in the infected root canal flora.

Bacteroidaceae Infections↗

PubMed2005

[Association of estrogen receptor gene polymorphisms and primary trigeminal neuralgia].

OBJECTIVE: To investigate the association of estrogen receptor (ER) gene polymorphism and primary trigeminal neuralgia. METHODS: By polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), ER gene polymorphism was analyzed in 20 trigeminal neuralgia (TR) patients and 20 control individuals, and the distribution of ER genotype was compared in TR group and control group. RESULTS: There was no significant difference in frequencies of allele and genotype in XbaI or PvuII polymorphism or XbaI with PvuII polymorphisms together between TR group and control group (P > 0.05). The genotypic distribution of Xx or PpXx in TR group was higher than control group, and it was contary to xx, ppxx or Ppxx in TR group and control group. CONCLUSION: XbaI or PvuII polymorphism may be related to TR. Women with PpXx genotype may be a dangerous factor to primary trigeminal neuralgia.

Female↗

PubMed2004

[Construction of eukaryotic expression plasmid pSecTag2B-msBlyS expressing mouse soluble B lymphocyte stimulator].

OBJECTIVE: The purpose of this study was to clone the soluble form of the mouse BlyS (msBlyS) and insert it into a eukaryotic expression vector pSecTag2B in order to further elucidat the antitumor activity induced by msBlyS expressed by the recombined plasmid pSecTag2B-msBlyS. METHODS: Full length cDNA of mouse soluble BlyS (msBlyS) was amplified by reverse transcription-PCR from total RNA of mouse spleen. The PCR product was ligated directly with linearized vector pCR2.1 supplied in the TA cloning kit. The recombined plasmid pCR2.1-msBlyS which was selected and identified using blue-white screening method and restriction map analysis and the purified original plasmid pSecTag2B were both cut by HindIII and EcoR I. The digested fragments were extracted and purified from low-melting temperature agarose and ligated by T4DNA ligase. The recombined plasmid pSecTag2B-msBlyS were isolated and identified by restricted endonuclease cutting and Sanger dideoxy DNA sequencing. RESULTS: The sequencing data indicated that inserted msBlyS gene had correct DNA sequence and orientation. CONCLUSION: Eukaryotic expression vector pSecTag2B. Expressing mouse BlyS have successfully been cloned. This will provide us an opportunity to do further research work on BlyS.

Animals↗