Biomedical subjects

Chunlin Hou

Publications and source records attributed to Chunlin Hou.

12 recordsLinked to original sources

PubMed2006

[Development of the tissue-autografting].

OBJECTIVE: To introduce the current situation and prospect of the tissue-autografting, such as the flaps, muscle flaps, and bone(periosteum) flaps, and its application in reparative and reconstructive surgery. METHODS: Based on our own experiences and combined with the review of the literature at home and abroad, the latest development of the tissue-autografting was analyzed. RESULTS: The femoral anterolateral flap, latissimus dorsi muscle flap, upper arm lateral flap, scap flap, temporal fascial flap and perforator flap are the frequently used in clinic. Of all the perforator flap had such advantages as better repair of the recipient sites and less damage of the donor site. Because of more advantages of the free myocutaneous flap transplantation, it substituted the free muscle transplantation. The latissimus dorsi muscle myocutaneous flap was the most frequently used in the transplant of the vessels, with preserved function of the thoracodorsal nerve or with repair of the defected tissues by the bridge. The most common donor sites of the bone were ribs, iliac bone, fibula and scapula, so the severe bone defects or the bone non-union, femoral head ischemic necrosis, and the bone graft from the tumor removal could be managed with the bones from those sites. CONCLUSION: The autografting in repairing the tissue defect has become one of the most important surgical techniques in reparative and reconstructive surgery.

Humans↗

PubMed2006

[Research advance in prevention of postoperative adhesion using carboxymethylcellulose].

OBJECTIVE: To study the prevention of postoperative adhesion using carboxymethylcellulose (CMC). METHODS: The literature was reviewed extensively, concerning the physical and chemical characters of CMC, the mechanism of preventing adhesion, the effect on healing of anastomoses, the experimental researches and the clinical application. RESULTS: CMC was a polysaccharide with favorable biocompatibility, biodegradation, stable physical and chemical characters, and it can prevent postoperative adhesion. CONCLUSION: CMC is with a wide outlook in prevention of postoperative adhesion.

Carboxymethylcellulose Sodium↗

PubMed2006

[Experimental study on the artificial bladder reflex arc established in therapy of flaccid bladder after spinal cored injury].

OBJECTIVE: To establish the artificial bladder reflex arc by the normal body reflex pathway above the horizon of spinal cord injury to reinnervate the flaccid bladder and restore bladder micturition function. METHODS: An intradural microanastomosis was performed on the L6 ventral root to the S2 ventral root. After axonal regeneration,the "patellar ligament-spinal cord center-bladder" reflex pathway was reestablished. A long-term function of the reflex arc was observed in the nerve electrophysiological experiment, detrusor electromyography experiment, and urodynamic testing 8 months after anastomosis. RESULTS: Trains of the stimuli (200 microV, 5 ms) in the left L6 dorsal root and the nerve at the anastomosized site resulted in motor evoked potential from the distal to the anastomosized site before and after the spinal cord was destroyed horizontally between S1 and S4 segment levels in 2 Beagle dogs. The figure and amplitude of the evoked potential were similar to those of the control and general stability which showed a noninterventional wave. The urodynamic test revealed a rapid increase of the bladder pressure and a minor increase in the abdominal pressure. This showed that the bladder detrusor mainly resulted in the pressure increase. The bladder pressure increased to 60% of the normal on average compared with the controls when resulted in the left L6 dorsal root and the nerve anastomosized site were stimulated. CONCLUSION: The long-term observation by the nerve electrophysiological experiment, detrusor electromyography experiment, and urodynamic test indicate that the new artificial reflex arc can be established successfully. The somatic motor axons can regenerate into the parasympathetic endoneurial tubes of the autonomic nerve.

Animals↗

PubMed2005

[Evaluation of biological safety of continuous carbon-fiber reinforced polyolefin as hard tissue repair].

OBJECTIVE: To evaluate the biological safety of continuous carbon-fiber reinforced polyolefin as hard tissue repair material. METHODS: Biocompatibility of the material was evaluated through hemolysis test, pyrogen test, skin irritation test, cytotoxicity test, ames test, in vitro chromosome aberration test, and bone marrow cells Micronuclei test. RESULTS: No obvious hemolysis, pyrogenic characteristics, sensitivity, cytotoxicity, and mutagenicity were observed. CONCLUSION: The continuous carbon-fiber reinforced polyolefin composite material is of good biological safety. It meets all the demand made by biological safety as hard tissue repair material.

Animals↗

PubMed2005

[Tissue flap transferring for wound repair of the clavicle].

OBJECTIVE: To investigate the result of tissue flap transferring for wound repair of the clavicle. METHODS: From 1994 to 2000, 3 patients (1 with clavicle osteosynthesis, 1 with chronic clavicle osteomyelitis, and 1 with radioactive ulcer in clavicular region accompanied by chronic osteomyelitis of clavicle) were reconstructed with turnover adipofascial flap, myocutaneous flap of pectoris, and myocutaneous flap of latissimus dorsal respectively. The outcome was observed. The operation principles of tissue flaps transferring for wound repair of the clavicle were summarized. RESULTS: Follow-ups were done for 2 months to 7 years. All tissue flaps survived well and the wounds in clavicular region were healed well. There was no recurrence of chronic clavicle osteomyelitis. CONCLUSION: Turnover adipofascial flap, myocutaneous flap of pectoris and latissimus dorsal are often used for wound repair of the clavicle. Most of the wounds of the clavicle can be repaired by turnover adipofascial flap. Myocutaneous flap of pectoris and latissimus dorsal are more suitable for wound repair with chronic clavicle osteomyelitis. In the case of radioactive ulcer of the clavicular region, myocutaneous flap of latissimus dorsal transposition is a better alternative for wound repair.

Adult↗

PubMed2005

[Turnover adipofascial flap for wound repair of the extremities].

OBJECTIVE: To introduce the clinical experience of local distally based turnover adipofascial flap for small to medium size wound of the extremities. METHODS: From 1994 to 2003, 33 cases of distally based longitudinal neuro-veno-adipofascial turnover adipofascial flap (axial perforator pattern in 26, random pattern in 7) were transferred in the forearm (19) and lower leg (14). These flaps were all raised in the forearm or lower leg as local flaps. The length (pedicle plus flap) was 9 to 18 cm and the width was 3 to 4 cm, with L/W ratio of 3 to 5 : 1. After transferring by 180 degrees turnover, a split-thickness skin graft was used to cover the fascia surface. RESULTS: All the 33 flaps survived. The donor sites were closed directly, and the recipient sites were covered with full- or split-thickness skin grafts. Both donor and recipient sites healed primarily. CONCLUSION: Turnover adipofascial flap is a simple and reliable method for small to medium size wound of the extremities.

Adolescent↗

PubMed2005

[Osteomyocutaneous latissimus dorsi scapular combined flap with vascularized rib to repair the large defect of tibia].

OBJECTIVE: To design a combined flap of subscapular axis including vascularized lateral scapular, rib and latissimus dorsi to repair the large defect of tibia. METHODS: The patient was a 39-year-old man who got a posttraumatic 12 cm defect of tibia after primary debridement and external fixation because of open fracture 5 months ago. There was a 12 cm x 6 cm scar involved the proximal medial segment of tibia. After resection of scar and fibular tissue over the bone defect floor, a latissimus dorsi myocutaneous flap 14 cm x 5 cm pedicled with subscapular artery-thoracodorsal artery, a flap 12.5 cm on the outside of the scapular pedicled with thoracodorsal artery, and 6th rib flap 13 cm by serratus were prepared. The tibialis posterior and saphenous vein were used for anastomosis. A proximal anatomic plate was applied to the fixation of tibia. RESULTS: The compound flap survived the operation. The follow-up period was 2 years. Bone union occurred 6 months after operation. CONCLUSION: This combined flap is successful and can provide alternative to the resolution of large defect of tibia.

Adult↗

PubMed2005

[Therapy progress of atonic bladder after spinal cord injury].

OBJECTIVE: To study the advance in the treatment of atonic bladder after spinal cord injury. METHODS: The recent literature concerned was extensively reviewed and some methods of therapy for atonic bladder were introduced. RESULTS: No effective method of therapy was found for atonic bladder after spinal cord injury. When compared with clean intermittent catheterization, pharmacologic therapy, compressive micturition and detrusor function reconstruction, the establishment of an artificial bladder reflex arc may have a potential of controllable micturition. CONCLUSION: To establish an artificial bladder reflex may provide an update of current therapeutic concepts for atonic bladder after spinal cord injury.

Humans↗

PubMed2004

[Research on carboxymethyl chitosan acting as the adjuvant for implantable degradable microspheres].

As a kind of biomaterial, carboxymethyl chitosan (CMC) has excellent biodegradable and bioacceptable capabilities using. This study was aimed to probe into the feasibility of CMC to prepare the implantable sustained release Ciprofloxacin Hydrochloride (CPX) microspheres(MS), and to go further into the pharmaceutic technology, the morphology and the characteristics of in vitro release of the microspheres. First, we prepared the microspheres by emulsification and cross-linking technology. Then, scanning electron microscopy (SEM), infrared spectrum (IR) and differential thermal analysis (DTA) were used to detect the structure and morphology of the MS. The in vitro release of CPX/CMC-MS and the CPX content of the MS were detected through continuous-flow releasing system. We found that the structure and morphology of the MS were affected by the conditions of preparation such as emulsification and cross-linking temperature, ionic strength and stirring speed, that the releasing time of CPX was more than 7 days, and that the releasing behaviors of the microspheres conformed to the Higuchi model. So we drew the conclusions that CMC could be used as a kind of absorbable and implantable adjuvant for sustained release, the technology of emulsification and cross-linking was proved to be feasible, stable and simple.

Absorbable Implants↗

PubMed2004

[Experimental and clinical studies on selective sacral rhizotomy in treatment of spastic bladder after spinal cord injury].

OBJECTIVE: To observe the effect of selective sacral rhizotomy in treating spastic bladder after spinal cord injury and to explore the mechanism and the best surgical method of different sacral rhizotomies. METHODS: The spastic bladder models were established in 12 male dogs and were divided into 4 groups according to the different rhizotomies of the sacral nerve as the following: rhizotomy of the anterior root of S2 (group A), rhizotomy of the anterior root of S2 and half of the anterior root of S3 (group B), rhizotomy of the anterior roots of S2 and S3 (group C), and total rhizotomy of the nerve roots of S2-4 (group D). By urodynamic examination and electrophysiological observation, the changes of all functional data were recorded and compared between pre-rhizotomy and post-rhizotomy to testify the best surgical method. In clinical trial, according to the results of the above experiments, rhizotomy of the anterior root of S2 or one of the half-anterior root of S3 were conducted on 32 patients with spastic bladder after spinal cord injury. The mean bladder capacity, the mean urine evacuation and the mean urethra pressure were (120 +/- 30), (100 +/- 30)ml and (120 +/- 20) cm H2O, respectively before rhizotomy. RESULTS: After rhizotomy, the bladder capacity in 4 groups amounted to (150 +/- 50), (180 +/- 50), (230 +/- 50), and (400 +/- 50) ml, respectively; and the urine evacuation volume were (130 +/- 30), (180 +/- 50), (100 +/- 50) and (50 +/- 30)ml, respectively. In the treated 32 patients, the mean bladder capacity were raised to 410 ml, and the mean urine evacuation volume were also increased to 350 ml. Incontinence of urine disappeared in all patients. After 22-month follow-up on 13 patients, no recurrence was observed. CONCLUSION: The effect of selective sacral rhizotomy in treating spastic cord injury is significant and worthy of further studies.

Adult↗

PubMed2002

Surgical management for sciatic nerve injury.

OBJECTIVE: To summarize surgical treatments and their corresponding curative effects on sciatic nerve injuries. METHODS: Surgical treatments on sciatic nerve injury were performed in 28 patients from January 1990 to July 2000. The treatments included neurolysis, neurolysis plus partial nerve anastomosis, nerve anastomosis and nerve transplantation. The curative effect was evaluated according to Sunderland criteria. RESULTS: Of 28 cases, 22 patients were followed up with a follow-up period of 13 months to 5 years (average 30 months). Of 22 nerves, 7 were excellent, 5 good, 7 fair and 3 poor, with an excellence rate of 54.5%. CONCLUSIONS: The fair results of sciatic nerve injury are related to its structural character. Surgical exploration should be performed if nerve function does not recover 3 months after primary operation and if Tinel's sign and electromyogram show no signs of nerve regeneration. Electrophysiological monitoring in the operation is useful in electing surgical methods and predicting the results of nerve anastomosis.

Adolescent↗

PubMed2002

[Bladder controller in bladder function reconstruction of paraplegic dogs].

OBJECTIVE: To observe whether bladder controller can restore bladder function in paraplegic dogs. METHODS: Four dogs were studied after their T(10) spinal cord was transected. Electrodes implanted around S(2) bilaterally were connected to subcutaneous recievers. Microsurgical techniques were employed in dorsal rhizotomy at S(1 - 3) intradural segment. After daily stimulation, the results of bladder controller were evaluated by micturition and vesicography. RESULTS: Micturition was given under electrical stimulation with a urine volume of 80 - 140 ml per time. The mode of micturition was post-stimulus voiding. Vesicography showed that the bladder was filled and bladder neck was open in the micturition course of electric stimulation. Residual urine volume was 15 - 20 ml. CONCLUSION: Bladder controller together with a sacral deafferentation procedure can restore bladder function of paraplegic dogs.

Animals↗