Biomedical subjects

Cornelia Amălinei

Publications and source records attributed to Cornelia Amălinei.

7 recordsLinked to original sources

PubMedDate not supplied

[New data about ITO cells].

Ito cells (the fat-storing cells) are stellate cells located in the space of Disse. They can be selectively stained with gold chloride, but their distinguishing feature in routine histological preparations is the presence of multiple lipid droplets in their cytoplasm. Ito cells have the capacity to accumulate exogenously administered vitamin A as retinyl esters in lipid droplets. The quantity of vitamin A, which is stuck into Ito cells, decreases progressively in liver injury, like chronic alcohol intake. Also, the fat-storing cells are the principal cellular source of collagen and other extracellular substances in normal as well as fibrotic liver. Liver injury is followed by an increase in the number of the fat-storing cells.

Adipocytes↗

PubMedDate not supplied

[Granular cells of the hepatic capillary sinuses--receptors].

Pit cells or hepatic natural killer (NK) cells represent an organ-associated NK cell population. They are situated in liver sinusoids and exert high spontaneous cytotoxic activity against tumor cell lines and may act as a primary defense barrier to metastasing tumor cells and to virus infections. Pit cells express two types of receptors on their cell membrane. One type activates NK cell killing (NCR or natural cytotoxicity receptors, as NKp46, NKp44, NKp30, NKG2D) by recognizing ill-defined molecules on target cells. The second type of receptors inhibits the lytic pathway by recognition of self class I MHC molecules and are represented by KIR or killer cell Ig-like receptors, as KIR2D, CD94/NKG2. Pit cells express on their cell membrane and other type of molecules as CD2, CD54, CD11a/CD18 and are CD3 negative.

Animals↗

PubMedDate not supplied

[Morphea associated with toxoplasmosis].

Circumscribed scleroderma or morphea is a rare disease that involves limited areas of skin and usually is not associated with visceral lesions. Its etiology is still debated, but its appearance signifies a particular immunological status. We present a case of a male patient, which developed morphea lesions during the infection with Toxoplasma gondii. Clinical manifestation was characteristic for the classic plaque-type of morphea. Diagnosis was confirmed by the histopathologic examine of the cutaneous lesions. The evolution of lesions was correlated with antibodies titre for Toxoplasma gondii, and local administration of corticosteroids accelerated the evolution to cutaneous lesions stabilization.

Administration, Cutaneous↗

PubMedDate not supplied

Aspects of the humoral and cellular immune response in hepatitis associated with cholelithiasis.

AIM: To investigate the immunological reactions of the liver in various gall bladder disease. METHODS: IgG, IgA and IgM were measured in the blood and bile of patients with cholecystectomy. Immunofluorescence and electron microscopy techniques were used. RESULTS: The tests showed high blood values of immunoglobulins and their presence in the bile. The immunofluorescence was positive for IgG in the hepatic sinusoids. The electron microscopy showed aspects of "cellular cooperation". CONCLUSIONS: On the basis of our results we can affirm that in cholelithiasis there exists an immune response to the persistent antigens, with the release of circulating immune complexes.

Bile↗

PubMedDate not supplied

Adenosquamous cervical carcinoma morphological characteristics.

Adenosquamous carcinomas range between 5-25% of cervical cancers and are composed by an admixture of malignant squamous and glandular elements. The aim of our study was to identify some common characteristics and to evaluate the correlation between the degrees of differentiation of the two components. We analyzed 15 cases diagnosed in a 6 years period. The age ranged between 26 years and 67 years (mean age 46.5 years). Paraffin embedding, followed by HE staining were performed. Differential diagnosis with endometrioid adenocarcinoma of the cervix with squamous metaplasia was made. Four cases (26.66%) were subtyped as clear cell adenosquamous carcinomas and 2 cases (13.33%) were subtyped as glassy cell carcinomas, exhibiting finely granular ground glass type cytoplasm. 93.33% of cases exhibited a poorly differentiated squamous component and 66.66% of cases exhibited a well differentiated glandular component. 20% (3 cases) presented prominent lymphoplasmacytic and eosinophilic inflammatory tumoral infiltrate. Squamous intraepithelial lesions in overlying epithelium was observed in 4 cases (26.66%). One case presented extension to the uterine body. One case, diagnosed as glassy cell subtype, presented regional lymph node metastases. Our study concluded the occurrence of adenosquamous cervical carcinomas at a similar age with squamous cervical carcinomas in the investigated group of patients. As adenosquamous cervical carcinomas are considered expressions of a biphasic differentiation of a single pluripotential sub-columnar reserve cell, a similar degree of differentiation of the two components would be expected. Although, we registered a degree of variability in grading of the two components, with a tendency of squamous component toward poorly differentiated aspect and a slightly dominant aspect of well differentiated glandular pattern.

Adult↗

PubMedDate not supplied

[Diagnostic difficulties in mammary gland adenosis. Case report].

Tumor adenosis represents a rare lesion, which most frequent appears as a palpable mass, with more than the usual dimensions. The paper presents a case of tumor adenosis of the mammary gland of a 16 years girl. Macroscopically, the tumor was well delimited, with gray-white surface and a nodular aspect on section. The tissue was processed by routine technique and stained with hematoxylin-eosine (H&E). There were also performed immunohistochemical (Actin SM, CK, S100) and special stainings (silver impregnation). Microscopically, there were observed several histologic types of adenosis, the most frequent being sclerosing adenosis. Through silver impregnation it was observed that the basement membrane, which surrounded the tubes, was intact. Immunohistochemically, there were evidentiated the epithelial and myoepithelial components of the tubes. The presence of these elements led through the diagnosis of tumor adenosis. The importance of recognition of the tumor adenosis is found in the possibility of wrong interpretation of this with tubular invasive carcinoma, these two entities being often mistaken, mainly at the frozen-section examination.

Adenocarcinoma↗

PubMedDate not supplied

[The detection of dysplastic lesions of the gallbladder mucosa in polypoid cholelithiasis].

AIM: To investigate the hyperplastic and/or dysplastic lesions in the gallbladder mucosa. METHODS: We used the immunohistochemical Ardeleanu-Hsu-Bussolati and Gugliotta methods, with avidin and peroxide biotin, adapted in the laboratory of University Hospital "St. Spiridon" Iaşi. There were studied ten cases of polypoid cholelithiasis, with the following markers: Ki67, PCNA, and p53. RESULTS: The immunohistochemical methods using Ki67, PCNA and p53 protein allowed the evaluation of the abnormal proliferation abilities characteristic for some hyperplastic or dysplastic lesions, found in our cases of cholelithiasis. CONCLUSIONS: The utilization of these markers permitted us to establish a good correlation between the high activity of Ki67 and the morphological aggression indicators in hyperplastic and dysplastic gallbladder epithelium. There is also a correlation between the percentage of the cells marked with anti-PCNA antibody and the existence of p53 protein in these lesions.

Biomarkers, Tumor↗