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Cortino Sukotjo

Publications and source records attributed to Cortino Sukotjo.

5 recordsLinked to original sources
PubMed2003

Oral fibroblast expression of wound-inducible transcript 3.0 (wit3.0) accelerates the collagen gel contraction in vitro.

Wounds of the oral mucosa show faster closure with less scar formation than skin wounds in other areas. A differentially expressed cDNA, wound-inducible transcript 3.0 (wit3.0), was isolated from oral mucosal wound in rats (Sukotjo, C., Abanmy, A. A., Ogawa, T., and Nishimura, I. (2002) J. Dent. Res. 81, 229-235). The purpose of this study was to characterize the wit3.0 gene structure and the function of its deduced peptide. Human and rat genome databases revealed that the gene for wit3.0 was located in human chromosome 12p11.23 and rat chromosome 4q44. Its human and rat gene structures were well conserved, composed of 7 exons spread over 20 kb. Exon 5 was alternatively spliced generating two transcripts encoding deduced peptides of 215 and 253 amino acids (wit3.0 alpha and wit3.0 beta, respectively). The protein families data base of alignments (Pfam) analysis suggested the wit3.0 peptide sequence shared similarity with a portion of the myosin II coiled-coil domain consensus sequence. Fibroblasts isolated from the rat oral wound up-regulated wit3.0 expression and exhibited greater ability to contract collagen gel in vitro than fibroblasts isolated from untreated oral mucosa/gingiva. NIH3T3 and rat oral fibroblasts transfected with expression vector containing the coding sequences of wit3.0 alpha or wit3.0 beta increased in vitro collagen gel contraction. When treated with TGF beta-1, NIH3T3 fibroblast expression of wit3.0 showed no significant change, whereas alpha smooth muscle actin was increased in a dose-dependent manner. These data suggest that there may be a novel wound healing pathway involving wit3.0 underlying the favorable early wound closure characteristics of oral mucosa.

Actins↗
PubMed2002

Modulated bone matrix-related gene expression is associated with differences in interfacial strength of different implant surface roughness.

PURPOSE: The objective of this study was to examine the effect of implant surface topography on biomechanical strength and expression patterns of bone extracellular matrix (ECM)-related genes during implant healing. MATERIALS AND METHODS: Cylinder implants with turned or dual acid-etched (DE) surface were placed into the femurs of female Sprague-Dawley rats. At 2 and 4 weeks after the implantation, the implant push-in test was performed to measure the load-bearing strength of the bone-tissue interface. T-shaped hollow implants were separately placed and the total RNA was extracted from the ingrown tissue within the hollow chamber. The RNA was subjected to reverse transcriptase polymerase chain reaction to analyze the expression pattern of selected bone extracellular matrix-related genes. RESULTS: The push-in value of the DE implant is approximately 300% greater than that of the turned implant at weeks 2 and 4 (p=.0090). The expression of collagen II, osteocalcin and biglycan was higher in the week 2 DE implant group than the turned implant group. At week 4 the expression of collagen II and IX was 7- and 26-fold, respectively, greater in the DE implant group than in the turned implant group. CONCLUSIONS: The surface topography of implants may induce the phenotypic alteration of wound healing cells. The increased interfacial strength of the DE implant may be associated with the modulated expression of the selected set of bone extracellular matrix genes.

Acid Etching, Dental↗