Recent studies on the estimation of human pituitary gonadotrophins in urine in clinical conditions.
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Biomedical subjects
Publications and source records attributed to D A Adamopoulos.
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The relationship of seminal plasma Mg, Ca and inorganic phosphate concentration to various parametres of seminal quality was evaluated in 41 normozoospermic and 102 dysspermic men with or without accessory gland infection. Ca concentration in asthenozoospermic and oligozoospermic patients was lower than in normozoospermic men and was positively correlated with Mg concentration irrespectively of seminal quality or the presence of infection. Mg concentration in normozoospermic or in oligozoospermic patients with infection was lower than in normozoospermic men without infection but higher in azoospermic men. Mg was negatively correlated to pH and both Mg and Ca concentrations were related to acid phosphatase levels. Inorganic P was higher in asthenozoospermic but lower in azoospermic patients than in normozoospermic men. In astheno-, oligo- and azoospermic men, P was positively correlated with fructose. In patients with accessory gland infection, successful treatment resulted in a significant concentration rise of all three elements. It is concluded that, from a diagnostic point of view, estimations of these elements, particularly of Mg and P, in seminal plasma reflect satisfactorily the functional capacity of the accessory glands of the genital system and the response to treatment in dysspermia associated with infection.
The effect of testosterone (T) on germinal epithelium development and maturation was studied in prepubertal male rabbits. For this purpose, implants of free T (50 mg) or placebo (PL) were placed intratesticularly in 55-60-day-old animals. Each group was divided into sub-groups additionally treated with 0.9% NaCl, FSH/LH (3.0 IU) or cyproterone acetate (CA, 2.5 mg) over a 45-day period. Initial and final evaluations included measurements of testicular volume, testicular biopsy score count (TBSC) and plasma T, FSH and LH concentrations. At the end of the experiment, increment values (final-initial evaluation) of the parameters examined showed the following differences: (1) mean TBSC in T-implanted, irrespective of additional treatment, was higher than that of PL-implanted rabbits (P less than 0.01); (2) mean plasma T, FSH and LH values were significantly different in T- and PL-implanted rabbits, with higher T (P less than 0.05) but lower FSH and LH increments (P less than 0.01 for both) in the former group; (3) marked differences amongst types of additional treatment, irrespective of the implant used, were found for TBSC, T, FSH and LH (P less than 0.01 for all); animals treated with CA had markedly lower increments than other treatment groups. From these findings, it may be concluded, that in the prepubertal rabbit T plays an important role in development and maturation of the testis, but this effect probably requires the synergistic action of endogenous gonadotrophins.