N-terminal ten amino acid sequence determined for B-cell differentiation factor derived from rabbit breast milk cell supernatant.
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Amino Acid Sequence↗
Biomedical subjects
D A Axelrod
Publications and source records attributed to D A Axelrod.
Polyclonal B-cell activation of rabbit Peyer's patch.
This study delineates the culture conditions and cellular requirements for pokeweed mitogen (PWM)-induced polyclonal B-lymphocyte activation of rabbit Peyer's patch cells for immunoglobulin secretion. This system was optimal with a cell density of 3 X 10(5) cells/ml and a culture duration of 7 days, in round-bottom containers. Although glass-plate-adherent cells did not appear to influence this process, the immunoglobulin responses to PWM were T cell dependent. This study supplies a foundation for further work on the mechanisms of polyclonal activation of B cells in the rabbit Peyer's patch.
Animals↗
Primary and secondary in vitro immune response of the rabbit Peyer's patch and spleen to RDEC-1 pili.
The culture conditions and cellular requirements for antigen-induced B-cell activation of the rabbit Peyer's patch and spleen using the RDEC-1 pilus antigen were delineated. It was found that optimal conditions for both primary and secondary in vitro immunization are similar except for culture duration. Optimal cell density was 3 X 10(6) cells/ml and optimal antigen dose was 30 to 100 ng/ml. Antibody production was best carried out in flat-bottom culture plates. Both T cells and glass-plate-adherent cells (macrophages) were required for antigen induced antibody production for both the primary and secondary responses. Of note was the inability to induce a primary antibody response in the spleen cell cultures. This study demonstrates the effectiveness of the system in elucidating some of the complex in vitro events associated with primary and secondary, antigen-specific B-cell activation of the rabbit Peyer's patch to RDEC-1 pili and the ability of rabbit spleen cell cultures to respond to RDEC-1 pilus antigen only after in vivo challenge.
Animals↗
Effects of nifedipine therapy on pulmonary Raynaud's in primary Sjögren's syndrome.
A case of Raynaud's phenomenon of the pulmonary vascular bed in Primary Sjögren's syndrome and subsequent response to calcium channel blockade is presented. Pulmonary Raynaud's phenomenon in Primary Sjögren's syndrome has not been previously described.
Adult↗
Rheumatoid factor isotypes following immunization.
Serum from 422 healthy subjects prior to and four weeks after multiple immunizations was studied for rheumatoid factors (RF) by an enzyme-linked immunosorbent assay (ELISA). Increase in IgM RF was detected in 22% of subjects by ELISA, but was not detectable by a standard sheep-cell agglutination test. Immunization may be a cause of RF positivity by a sensitive assay such as ELISA; it is not likely to be detected by standard clinical methods.
Adult↗