D A Gooray | PubMed Health

PubMed1986

Pneumoperitoneum associated with mechanical ventilation.

The true incidence of pneumoperitoneum associated with mechanical ventilation is unknown, but current estimates range from rare to 7 percent of intubated intensive care unit patients. This syndrome should be strongly suspected when pneumoperitoneum develops in a mechanically ventilated patient with a combination of (1) peak inspiratory pressure (PIP) greater than 40 cm H(2)O; (2) positive end expiratory pressure (PEEP) greater than 6 cm H(2)O; and (3) coexistent evidence of significant pulmonary barotrauma. An illustrative case report is presented where this entity was recognized and the patient managed without laparotomy. Controlled prospective studies should determine the true importance of this problem.

Adolescent↗

PubMed1978

Determination of 8-methoxypsoralen in plasma by scanning fluorometry after thin-layer chromatography.

A rapid and sensitive method is described for determining 8-methoxypsoralen in plasma. Plasma samples are acidified with 6 mol/liter and heated in a boiling water bath to release the plasma-bound drug nondestructively. It then is extracted into a solvent mixture consisting of benzene/ethyl acetate (9/1 by vol). The solvent phase is separated, evaporated, and an aliquot of the dissolved residue is thin-layer chromatographed, with benzene/ethyl acetete (9/1) as developing solvent. The plate is dried and the spots, made visible under ultraviolet light (320-400 nm), are scanned. The smallest amount detectable is 20 ng; the overall analytical recovery from plasma is 84%. We used the method to determine the drug in the plasma of rabbits after intravenous and oral administration of 10 mg, and in one patient after an oral dose of 30 mg.

Animals↗