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D A Hopkins

Publications and source records attributed to D A Hopkins.

At least 19 recordsLinked to original sources
PubMed1995

Monoamines in the parabrachial nucleus of the cardiomyopathic hamster.

The development of dystrophic cardiac muscle is related to increases in sympathetic nervous system activity but little is known regarding possible central neural mechanisms that may be involved in cardiomyopathy. The inbred cardiomyopathic hamster is an animal model for studying the development and mechanisms of necrosis in cardiac muscle which resemble non-vascular myocardial diseases of man. Because monoamines are known to play a major role in central regulation of the cardiovascular system, we compared the distribution and density of tyrosine hydroxylase (TH) and 5-hydroxytryptamine (5-HT) immunostaining in the brains of cardiomyopathic hamsters (strain CHF-146), a related strain (CHF-148) of non-cardiomyopathic albino hamsters, and golden Syrian hamsters for possible differences in neurochemical organization. At the time of sacrifice, the cardiomyopathic hamsters exhibit small, calcified lesions on the surface of the ventricular cardiac muscle (early necrotic phase). Brain sections from each group were processed identically and simultaneously. The results show that there were significant differences among strains in the parabrachial nucleus with respect to the two neurochemicals examined. In golden Syrian and albino hamsters, TH and 5-HT immunoreactive axons were lightly-to-moderately stained in the lateral parabrachial nucleus. In the cardiomyopathic hamster, there were significantly more densely stained TH and 5-HT immunoreactive axons in the lateral parabrachial nucleus, in particular the inner part of the external lateral subnucleus. Because the lateral parabrachial nucleus, including the external lateral subnucleus, is known to be involved in regulation of the cardiovascular system, the differential distribution of TH and 5-HT in the parabrachial nucleus of cardiomyopathic hamsters in comparison to normal hamsters suggests that the parabrachial nucleus could be involved in sympathetic mechanisms related to the development of necrosis in cardiac muscle of the cardiomyopathic hamster.

Animals↗
PubMed1995

Nitric oxide modulates signaling between cultured adult peripheral cardiac neurons and cardiomyocytes.

To determine whether nitric oxide (NO) modifies cardiomyocytes directly or indirectly via peripheral autonomic neurons, the effects of NO were studied in long-term (3-6 wk) cultures of adult guinea pig ventricular myocytes alone as well as in cocultures with adult extracardiac (stellate ganglion) or intrinsic cardiac neurons. NADPH diaphorase was associated histochemically with cultured intrinsic cardiac and, to a lesser extent, stellate ganglion neurons. The beating frequency of ventricular myocytes cocultured with intrinsic cardiac neurons (M-intrinsic) or stellate ganglion neurons (M-stellate) increased by 20-30% (P < 0.001) after administration of the NO donor S-nitroso-N-acetylpenicillamine (SNAP); this effect was abolished by the guanylate cyclase inhibitor LY-83583. The beating frequency of noninnervated myocyte cultures was not affected by SNAP. The precursor of NO, L-arginine, also increased the beating rate (approximately 20%; P < 0.05) of M-intrinsic cocultures, not affecting that of M-stellate cocultures or noninnervated myocyte cultures. Augmentor effects induced by SNAP were no longer elicited in the presence of tetrodotoxin and were unaffected by beta-adrenergic or muscarinic receptor blockade. It is concluded that 1) NO-sensitive neurons are present in stellate and intrinsic cardiac ganglia, and these neurons increase the beating rate of cardiomyocytes in the presence of NO; 2) more NO-synthesizing neurons are present in M-intrinsic than M-stellate cocultures, since L-arginine increased the beating frequency of myocytes significantly only in M-intrinsic cocultures; and 3) the beating rate of noninnervated myocyte cultures is not directly affected by NO.

Animals↗
PubMed1995

Modulation of intrinsic cardiac neuronal activity by nitric oxide donors induces cardiodynamic changes.

Studies were performed to determine 1) whether a specific marker for nitric oxide production is associated with canine intrinsic cardiac neurons, 2) whether the transmembrane properties of these neurons can be altered by nitric oxide donors, 3) whether in situ intrinsic cardiac neurons are sensitive to nitric oxide donors, and 4) whether these neurons are involved in cardiac regulation. Thirty to forty percent of canine intrinsic cardiac neurons were labeled with a selective anatomic marker for nitric oxide production. Nitric oxide donors modified the transmembrane properties of a subpopulation of intrinsic cardiac neurons studied in vitro. The nitric oxide donors nitroglycerine, sodium nitrite, and nitroprusside induced concentration-dependent increases in neuronal activity frequently associated with cardiac augmentation. Similar neuronal responses were elicited by N-methyl-D-aspartate receptor activation as well as when the precursor of nitric oxide, L-arginine, and the exogenous nitric oxide donor, S-nitroso-N-acetylpenicillamine, were administered, indicating that intrinsic cardiac neurons can be modulated by nitric oxide donors. Such neurons apparently are tonically influenced by locally released nitric oxide as local administration of the competitive inhibitor of nitric oxide synthase, NG-nitro-L-arginine methyl ester, suppressed their spontaneous activity. These data indicate that a significant population of nitric oxide-sensitive neurons exists in the canine intrinsic cardiac nervous system that are involved in cardiac regulation.

Animals↗
PubMed1994

Gross and microscopic anatomy of the canine intrinsic cardiac nervous system.

BACKGROUND: A three-dimensional description of the distribution and organization of the canine intrinsic cardiac nervous system was developed in order to characterize its full extent physiologically. METHODS: The anatomy of the canine intrinsic cardiac nervous system was investigated in 67 mongrel dogs by means of visualization following methylene blue staining as well as by light and electron microscopic analyses. RESULTS: Collections of ganglia associated with nerves, i.e., ganglionated plexuses, were identified in specific locations in epicardial fat and cardiac tissue. Distinct epicardial ganglionated plexuses were consistently observed in four atrial and three ventricular regions, with occasional neurons being located throughout atrial and ventricular tissues. One ganglionated plexus extended from the ventral to dorsal surfaces of the right atrium. Another ganglionated plexus, with three components, was identified in fat on the left atrial ventral surface. A ganglionated plexus was located on the mid-dorsal surface of the two atria, extending ventrally in the interatrial septum. A fourth atrial ganglionated plexus was located at the origin of the inferior vena cava extending to the dorsal caudal surface of the two atria. On the cranial surface of the ventricles a ganglionated plexus that surrounded the aortic root was identified. This plexus extended to the right and left main coronary arteries and origins of the ventral descending and circumflex coronary arteries. Two other ventricular ganglionated plexuses were identified adjacent to the origins of the right and left marginal coronary arteries. Intrinsic cardiac ganglia ranged in size from ones comprising one or a few neurons along the course of a nerve to ones as large as 1 x 3 mm estimated to contain a few hundred neurons. Intrinsic cardiac neuronal somata varied in size and shape, up to 36% containing multiple nucleoli. Electron microscopic examination demonstrated typical autonomic neurons and satellite cells in intrinsic cardiac ganglia. Many of their axon profiles contained large numbers of clear, round, and dense-core vesicles. Asymmetrical axodendritic synapses were common. CONCLUSIONS: The canine intrinsic cardiac nervous system contains a variety of neurons interconnected via plexuses of nerves, the distribution of which is wider than previously assumed.

Animals↗
PubMed1994

Chicken interferon gene: cloning, expression, and analysis.

A gene encoding chicken interferon (ChIFN) was cloned from a cDNA library made from primary chick embryo cells that had been "aged" in vitro so as to produce copious amounts of IFN upon induction. The coding region is predicted to produce a signal peptide of 31 amino acids and a mature protein of 162 amino acids with a molecular weight of 18,957. There are four potential N-glycosylation sites and six cysteine residues. Three disulfide bonds are possible, with two being common to most mammalian type I IFNs. A motif of 10 amino acids surrounding Cys-137 is highly conserved: It shows 80% homology with mammalian type I IFNs, but only 30% with a reported fish IFN. The T-rich 3' UTR displays the canonical element AATAAA required for polyadenylation, and contains six repeats of the octamer CTATTTAT that may be involved in down-regulating translation. Northern blots demonstrate that the accumulation of ChIFN mRNA correlates with induction of ChIFN determined by bioassay. Biologically active protein was synthesized in transfected mouse L cells using mRNA prepared in vitro from the cloned sequence. This activity was neutralized by a monoclonal antibody prepared against purified ChIFN. The ChIFN gene shows sequence identity at the amino acid/nucleotide level with consensus mammalian IFNs as follows: alpha (24/23%), beta (20/24%), omega (23/43%), tau (20/43%), gamma (3/31%), and with flatfish IFN (16/35%). The conserved features of the predicted ChIFN protein and the general similarity of predicted secondary structure suggest a molecule that fits the five alpha-helix three-dimensional topology reported for type I mammalian IFNs.(ABSTRACT TRUNCATED AT 250 WORDS)

Amino Acid Sequence↗
PubMed1992

Electrophysiological properties of in vitro intrinsic cardiac neurons in the pig (Sus scrofa).

Physiological properties and synaptically mediated responses of 34 ganglionated plexus neurons from the right atrium of the pig heart were studied with in vitro intracellular recording techniques. Whole-cell input resistance of these neurons was lower, time constant was shorter, and threshold for directly evoked action potentials was higher than the same properties in extracardiac autonomic neurons. Long intracellular depolarizing current pulses (400-500 ms) failed to generate more than one or two action potentials. Nicotinic and non-nicotinic synapses were present on neurons in cardiac ganglia and neuronal properties could be modified by norepinephrine. Based on their physiological properties, cardiac ganglionated plexus neurons in the pig appear to represent a distinct population of autonomic neurons that may be capable of intracardiac integration of efferent information to the heart.

Animals↗
PubMed1991

Activity of in vivo atrial and ventricular neurons in chronically decentralized canine hearts.

The spontaneous activity of 175 neurons located in the ventral right atrial and ventral interventricular ganglionated plexi was recorded in eight anesthetized dogs, the hearts of which were chronically decentralized at least 2 wk before recordings were made. Ganglia were subsequently identified anatomically in the immediate vicinity of the recording sites. Spontaneous activity was correlated with the cardiac cycle in 57% of the atrial and 62% of the ventricular neurons and with the respiratory cycle in 29% of the atrial and 28% of the ventricular neurons. Spontaneous cardiovascular-related activity was recorded when systemic arterial pressure was between 80 and 175 mmHg. The activity of 23 atrial and 15 ventricular neurons was altered when discrete regions of the heart were mechanically distorted by gentle touch. These data imply that cardiac ganglionated plexi contain afferent neurons that receive inputs from limited regions of the heart. The spontaneous activity generated by intracardiac neurons was not altered when extracardiac tissues were distorted. These results demonstrate that neurons in ganglia on chronically decentralized hearts can generate spontaneous activity, a large fraction of which is correlated with cardiovascular or respiratory events.

Adipose Tissue↗
PubMed1990

Topography and synaptology of mamillary body projections to the mesencephalon and pons in the rat.

The anterograde and retrograde transport of horseradish peroxidase conjugated to wheat germ agglutinin (WGA-HRP) was used to study the anatomical organization of descending projections from the mamillary body (MB) to the mesencephalon and pons at light and electron microscopic levels. Injections of WGA-HRP into the medial mamillary nucleus resulted in dense anterograde and retrograde labeling in the ventral tegmental nucleus, while injections in the lateral mamillary nucleus resulted in dense anterograde labeling in the dorsal tegmental nucleus pars dorsalis and dense anterograde and retrograde labeling in the pars ventralis of the dorsal tegmental nucleus. Anterogradely labeled fibers in the mamillotegmental tract diverged from the principal mamillary tract in an extensive dorsocaudally oriented swath of axons which extended to the dorsal and ventral tegmental nuclei, and numerous axons turned sharply ventrally and rostrally to terminate topographically in the dorsomedial nucleus reticularis tegmenti pontis and rostromedial pontine nuclei. The anterograde labeling in these two precerebellar relay nuclei was distributed near the midline such that projections from the lateral mamillary nucleus terminated mainly dorsomedial to the terminal fields of projections from the medial mamillary nucleus. In the dorsal and ventral tegmental nuclei, labeled axon terminals contained round synaptic vesicles and formed asymmetric synaptic junctions primarily with small diameter dendrites and to a lesser extent with neuronal somata. A few labeled terminals contained pleomorphic vesicles and formed symmetric synaptic junctions with dendrites and neuronal somata. Labeled axon terminals were also frequently found in synaptic contact with retrogradely labeled dendrites and neuronal somata in the dorsal and ventral tegmental nuclei. These findings indicate that neurons in the dorsal and ventral tegmental nuclei are reciprocally connected with MB projection neurons. In the nucleus reticularis tegmenti pontis and medial pontine nuclei, labeled axon terminals contained round synaptic vesicles and formed asymmetric synaptic junctions primarily with small diameter dendrites. The present study demonstrates that projections from the medial and lateral nuclei of the MB are topographically organized in the mesencephalon and pons. The synaptic morphology of mamillotegmental projections suggests that they may have excitatory influences primarily on the distal dendrites of neurons in these brain regions.

Afferent Pathways↗
PubMed1990

Activity of in vivo canine ventricular neurons.

The spontaneous activity of 113 neurons located in the ganglionated plexus in the epicardial fat overlying the cranial portion of the ventral intraventricular groove and the origin of the circumflex coronary artery was recorded in ten anesthetized dogs. Ganglia that contained varying numbers of neurons, some with two or more nucleoli, were subsequently identified anatomically in the vicinity of the recording sites. Spontaneous activity was correlated with the cardiac cycle in 81% and with the respiratory cycle in 17% of the identified neurons. The spontaneous cardiovascular-related activity occurred in relation to specific phases of the cardiac cycle when arterial pressure was between approximately 80 and 175 mmHg. When systolic pressures fell below approximately 80 mmHg or increased above approximately 175 mmHg, neurons displaying cardiovascular-related activity were inactive. The activity of 62% of all identified neurons was altered when discrete regions of the heart or pulmonary tissue were mechanically distorted by gentle touch. In many instances mechanical distortions of tissues were still able to alter neuronal activity following acute decentralization. Single stimuli or trains of stimuli delivered to the vagosympathetic complexes, stellate ganglia, or cardiopulmonary nerves generated bursts of activity in ganglionic neurons. Spontaneous activity occurred whether the ganglia were connected to the central nervous system or acutely decentralized. It is concluded that some neurons located on the canine ventricle display spontaneous activity that is related to cardiovascular or respiratory dynamics. The results also demonstrate that ventricular neurons can be influenced by sympathetic or parasympathetic efferent axons as well as cardiac mechanoreceptors.

Adipose Tissue↗
PubMed1990

Activity of canine in situ left atrial ganglion neurons.

The responses of 135 spontaneously active neurons were recorded from ganglionated plexi located in the three epicardial fat pads on the ventral surface of the left atrium of ten dogs. Ganglia, some of which were adjacent to the recording sites, containing varying numbers of neurons were identified throughout these fat pads. Spontaneous activity in 50% of the identified neurons was correlated with specific phases of the cardiac cycle when arterial systolic pressure was between approximately 70 and 180 mmHg and in 28% it was correlated with the respiratory cycle. More neurons displaying cardiovascular-related activity were recorded when systolic pressure was increased after administration of positive inotropic agents or aortic occlusion. However, when systolic pressure increased above approximately 150 mmHg the number of active neurons decreased, and when pressure reached approximately 180 mmHg no activity was recorded. The activity of 36% of identified neurons was altered when discrete regions of the heart, great thoracic vessels, lungs, neck, upper limb, chest wall, or abdominal wall were mechanically distorted by gentle touch. After acute decentralization of the intrathoracic nervous system some neurons still displayed spontaneous cardiovascular- or respiratory-related activity. Single stimuli or trains of stimuli delivered to the vagosympathetic complexes, stellate ganglia, or cardiopulmonary nerves activated neurons in intact or acutely decentralized preparations. It is concluded that ventral left atrial ganglionated plexi neurons display activity related to cardiovascular or respiratory dynamics, and that these neurons are influenced by sympathetic and parasympathetic efferent axons, as well as by cardiac and other mechanoreceptors.

Adipose Tissue↗
PubMed1990

Cardiac responses to electrical stimulation of discrete loci in canine atrial and ventricular ganglionated plexi.

The purpose of the present study was to examine cardiac effects induced by electrical stimulation (1-4 V, 1 ms, 200 Hz) of discrete loci within the ganglionated plexi located on canine atria and ventricles. When 20 loci in the right atrial ventral ganglionated plexi of 11 anesthetized open-chest dogs were stimulated, bradycardia and/or right and left atrial force suppression occurred when, on average, 15% of these loci were stimulated. Bradycardia and atrial force suppression were elicited when, on average, 8% of 15 loci in the left atrial ventral ganglionated plexi of eight dogs was stimulated. When these loci were restimulated after acute decentralization, cardiac responses were attenuated or occasionally eliminated. After atropine (1 mg/kg iv) administration, repeat stimulation of loci in the right but not left atrial ganglionated plexus induced tachycardia. Stimulation of loci in the right ventricular ganglionated plexus after the subsequent administration of desipramine (1 mg/kg iv) in six dogs resulted in an increase in right ventricular conus intramyocardial pressure. After hexamethonium administration (10 mg/kg iv, followed by a continuous infusion of 1 mg.kg-1.min-1), sympathetic responses were no longer elicited from one of the five dogs in which loci in the right atrial ganglionated plexi and from two of the six dogs in which loci of the right ventricular ganglionated plexus had elicited responses. We conclude that atrial and ventricular ganglionated plexi contain efferent parasympathetic, efferent sympathetic, and afferent neurons.

Animals↗
PubMed1989

Mamillary body in the rat: topography and synaptology of projections from the subicular complex, prefrontal cortex, and midbrain tegmentum.

The retrograde and anterograde transport of horseradish peroxidase conjugated to wheat germ agglutinin (WGA-HRP) has been used to trace afferent connections of the rat mamillary body (MB) at the light and electron microscopic levels. Injections of WGA-HRP into different parts of the MB resulted in heavy retrograde labeling in the subicular complex, medial prefrontal cortex, and dorsal and ventral tegmental nuclei. Injections of WGA-HRP into each of these brain regions, respectively, resulted in anterograde labeling with specific distributions and characteristic synaptic organizations in the MB. Projections from the rostrodorsal and caudoventral subiculum terminated in a topographically organized laminar fashion in the medial mamillary nucleus bilaterally, whereas afferent projections from the presubiculum and parasubiculum terminated only in the lateral mamillary nucleus. Labeled axon terminals which originated from the subicular complex were characterized by round vesicles and formed asymmetric synaptic junctions with small-diameter dendrites and dendritic spines in the medial and lateral mamillary nuclei. Projections from the prefrontal cortex originated mainly in the infralimbic area and to a lesser degree in the prelimbic and anterior cingulate areas. Injections of tracer into these brain regions gave rise to dense labeling of axon terminals in the medial mamillary nucleus, pars medianus, and in the anterior dorsomedial portion of the pars medialis. The labeled terminals were characterized by round vesicles and formed asymmetric synaptic junctions with small-diameter dendrites and dendritic spines. Projections from the dorsal tegmental nucleus terminated in the ipsilateral lateral mamillary nucleus, whereas afferent projections from the anterior and posterior subnuclei of the ventral tegmental nucleus terminated topographically in the medial mamillary nucleus. The ventral tegmental nucleus, pars anterior projected to the midline region of the medial nucleus and the dorsolateral and ventromedial subdivisions of the pars posterior projected to medial and lateral parts of the medial nucleus, respectively. In contrast to the synaptic morphology of subicular complex and medial prefrontal cortex axon terminals in the MB, labeled axon terminals in the MB which originated from the midbrain tegmentum were characterized by pleomorphic vesicles and formed symmetric synaptic junctions with neuronal somata and proximal dendrites as well as distal dendrites and dendritic spines.(ABSTRACT TRUNCATED AT 400 WORDS)

Afferent Pathways↗
PubMed1989

Viscerotopic representation of the upper alimentary tract in the rat: sensory ganglia and nuclei of the solitary and spinal trigeminal tracts.

The aim of this study was to map the viscerotopic representation of the upper alimentary tract in the sensory ganglia of the IXth and Xth cranial nerves and in the subnuclei of the solitary and spinal trigeminal tracts. Therefore, in 172 rats 0.5-65 microliters of horseradish peroxidase (HRP), wheat germ agglutinin-HRP, or cholera toxin-HRP were injected into the trunks and major branches of the IXth and Xth cranial nerves as well as into the musculature and mucosa of different levels of the upper alimentary and respiratory tracts. The results demonstrate that the sensory ganglia of the IXth and Xth nerves form a fused ganglionic mass with continuous bridges of cells connecting the proximal and distal portions of the ganglionic complex. Ganglionic perikarya were labeled in crude, overlapping topographical patterns after injections of tracers into nerves and different parts of the upper alimentary tract. After injections into the soft palate, pharynx, esophagus, and stomach, anterograde labeling was differentially distributed in distinct subnuclei in the nucleus of the tractus solitarius (NTS). Palatal and pharyngeal injections resulted primarily in labeling of the interstitial and intermediate subnuclei of the NTS and in the paratrigeminal islands (PTI) and spinal trigeminal complex. Esophageal and stomach wall injections resulted in labeling primarily of the subnucleus centralis and subnucleus gelatinosus, respectively. The distribution of upper alimentary tract vagal-glossopharyngeal afferents in the medulla oblongata has two primary groups of components, i.e., a viscerotopic distribution in the NTS involved in ingestive and respiratory reflexes and a distribution coextensive with fluoride-resistant acid-phosphatase-positive regions of the PTI and spinal trigeminal nucleus presumably involved in visceral reflexes mediated by nociceptive or chemosensitive C fibers.

Animals↗
PubMed1989

Ganglionic distribution of afferent neurons innervating the canine heart and cardiopulmonary nerves.

The ganglionic distribution of the perikarya of afferent axons in cardiopulmonary nerves or the heart was studied in 64 dogs by injecting horseradish peroxidase into physiologically identified cardiopulmonary nerves or different regions of the heart. In 6 additional dogs, horseradish peroxidase was injected into the aortic arch, pericardial sac, left ventricular cavity or the skin. After injections into cardiopulmonary nerves, retrogradely labeled perikarya were found in the ipsilateral nodose ganglion and the ipsilateral C7-T7 dorsal root ganglia. After injections into different regions of the heart, retrogradely labeled neurons were found in the nodose ganglia bilaterally and in the C6-T6 dorsal root ganglia bilaterally. Many more retrogradely labeled neurons were found in the nodose ganglia in comparison to the dorsal root ganglia. The largest numbers of retrogradely labeled perikarya in the dorsal root ganglia occurred in the T 2-4 ganglia following nerve or heart injections. Following injections into specific regions of the heart or individual physiologically identified cardiopulmonary nerves, regional distributions of labeled neurons could not be identified within or among ganglia with respect to the structures injected. Perikarya in dorsal root ganglia which were labeled after heart injections ranged in area from 436-3280 microns 2 (X = 1279 +/- 51 S.E.M.) while after skin injections labeled perikarya ranged in area from 224-5701 microns 2 (X = 1631 +/- 104 S.E.M.). The results show that the afferent innervation of the canine heart is provided by neurons located throughout the nodose ganglia and to a lesser degree in the C6-T6 dorsal root ganglia bilaterally. The bilateral distribution of cardiac afferent neurons raises questions regarding mechanisms underlying unilateral symptoms frequently associated with heart disease.

Animals↗
PubMed1988

Mamillary body in the rat: a cytoarchitectonic, Golgi, and ultrastructural study.

The present study provides a comprehensive light and electron microscopic analysis of the anatomical organization of the rat mamillary body. The cytoarchitecture and morphology of mamillary neurons were investigated with the aid of Nissl-stained and Golgi-impregnated sections cut in transverse, horizontal, and sagittal planes. The ultrastructural features of the mamillary nuclei were correlated with observations made on Golgi material. The mamillary body is comprised of a lateral and a medial nucleus, the latter being subdivided into five major subnuclei: pars lateralis, pars basalis, pars medialis, pars medianus, and pars posterior. The perikarya are medium-sized or small with the proportions of each differing among subnuclei. The largest perikarya are found in the lateral mamillary nucleus (cell area 257.0 microns2) and have 2-5 radially oriented aspiny dendrites that are often beaded. Small cells predominate in the pars lateralis (cell area 116.3 microns2) and pars basalis (cell area 118.3 microns2), whereas the pars medialis (cell area 196.7 microns2), pars medianus (cell area 136.5 microns2), and pars posterior (cell area 154.6 microns2) contain mainly medium-sized cells. The dendrites of most cells in the medial nucleus are radially oriented and exhibit a variety of spines including numerous short stubby spines, spines with thin necks that end in spherical swellings, and long thin spines. Neuronal somata are often closely apposed with no intervening glial processes and contain eccentrically located nuclei with one or more invaginations of the nuclear envelope. Two main classes of axon terminals were identified in the mamillary body. One type contains round vesicles and forms asymmetric synaptic junctions (RA) with dendrites and dendritic spines. RA terminals rarely contact neuronal somata and proximal dendrites in the MB. The second type contains pleomorphic vesicles and forms mainly symmetric synaptic junctions (PS) with neuronal somata as well as dendrites and spinous processes. Dense-cored vesicles were frequently seen in both types of terminals. Both types of terminals often synapse with two adjacent dendrites and are also found near or adjacent to each other on the same dendrite. A quantitative analysis indicated that the numbers of RA terminals in the medial nucleus almost equals the numbers of PS terminals, whereas the lateral mamillary nucleus contains considerably more PS (64%) than RA terminals (36%).

Animals↗
PubMed1988

Activity of in vivo canine cardiac plexus neurons.

The activity of 394 spontaneously active neurons located in the ganglionated plexus of the ventral epicardial fat pad overlying the right atrium and pulmonary veins was recorded. Ganglia that contained various numbers of neurons, many with two or more nucleoli, were identified adjacent to the recording sites. Spontaneous activity was correlated with the cardiac cycle in 39% and with the respiratory cycle in 8% of the identified neurons. Neuronal activity occurred in specific phases of the cardiac cycle when arterial pressure was between approximately 70 and 175 mmHg. During increases in systolic pressure induced by positive inotropic agents or aortic occlusion, responses of neurons that displayed cardiovascular-related activity were enhanced. These responses persisted after acute decentralization. The activity of 14% of all identified neurons was altered when discrete regions of the heart, great thoracic vessels, or lungs were mechanically distorted by gentle touch. Trains of stimuli, but not single stimuli, delivered to the vagosympathetic complexes, stellate ganglia, or cardiopulmonary nerves activated ganglionic neurons in intact or acutely decentralized preparations. It is concluded that the activity of some cardiac ganglion neurons is related to cardiovascular or respiratory dynamics and that some of these neurons receive inputs from sympathetic and parasympathetic efferent axons as well as from cardiac mechanoreceptors.

Adipose Tissue↗
PubMed1987

Viscerotopic representation of the upper alimentary tract in the medulla oblongata in the rat: the nucleus ambiguus.

The nucleus ambiguus has been reported to innervate various thoracic and abdominal viscera in addition to the musculature of the upper alimentary tract. However, the literature is contradictory as to how different regions of the nucleus ambiguus innervate specific organs. Therefore, a systematic investigation of the viscerotopic organization of the nucleus ambiguus was undertaken. In 102 rats, 0.5-10.0 microliter of HRP, WGA-HRP, cholera toxin-HRP or fluorescent tracers were injected into the IXth, Xth, and XIth cranial nerves and the major branches of the Xth as well as organs supplied by them. The results demonstrate that the nucleus ambiguus in the rat is made up of two major longitudinal divisions: a dorsal division comprised of three rostrocaudally aligned subdivisions representing the special visceral efferent component, and a ventral division comprised of at least two subdivisions representing the general visceral efferent component. The dorsal division corresponds to the nucleus ambiguus in the narrow sense and comprises a rostral esophagomotor compact formation, an intermediate pharyngolaryngomotor semicompact formation, and a caudal laryngomotor loose formation. Each of these formations displays a characteristic dendroarchitecture. The stylopharyngeal and cricothyroid motoneurons are displaced rostrad from the main pharyngeal and laryngeal motoneuronal pools. Thyropharyngeal (lower constrictor) motoneurons occupy the rostral half of the semi-compact formation and hyopharyngeal (middle constrictor) motoneurons its entire length. The ventral division of the nucleus ambiguus corresponds to the external formation, extends along the entire length of the medulla oblongata, and contains preganglionic neurons innervating the heart and supradiaphragmatic structures innervated by the glossopharyngeal and the superior laryngeal nerves.

Animals↗