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Biomedical subjects

D A Hunt

Publications and source records attributed to D A Hunt.

23 records · Page 2Linked to original sources
PubMed1980

Work induced myocardial glycogen resynthesis.

Work induced myocardial glycogen repletion was studied in an isolated perfused heart preparation. After 15 min of low or high workload perfusion with substrate free buffer, glycogen was depleted by 29% (p < .05) and 61% (p < .001) respectively. During the resynthesis phase with 11 mM glucose as substrate, glycogen was increased by 23% (p < .05) and 44% (p < .01) for the moderately and severely depleted hearts. The rate of glycogen resynthesis was 2.4 fold greater in the more severely depleted hearts. Providing the substrate depleted myocardium with 5.5 mM, 11 mM or 22 mM glucose during the recovery period resulted in glycogen resynthesis of 9.01, 9.95 and 16.53 mg/hr/gm dry wt. When severely depleted hearts were provided with 11 mM glucose or a combination of 11 mM glucose and 5 mM sodium pyruvate, glycogen repletion was 9.95 and 16.50 mg/hr/gm dry wt. These experiments indicate that an isolated perfused heart can be used to study work induced glycogen repletion which is responsive to a change in the preceding workload, substrate concentration and the availability of an alternative carbon source.

Animals↗
PubMed1978

Comparison of two rapid test procedures with the standard EC test recovery of fecal coliform bacteria from shellfish-growing waters.

A study was conducted to compare recovery and enumeration capability of two 24-hr multitube fermentation tests with the standard EC test for fecal coliform levels in shellfish-growing waters. The 2 tests were the A-1 test developed by Andrews and Presnell, specifying 24-hr incubation in A-1 medium at 44.5 degree C; and a modification of the A-1 test requiring a 3-hr resuscitation of 35 degree C before incubation at 44.5 degree C for 21 hr. Fifteen State, Federal, and Provincial laboratories examined 10 routine shellfish-growing area samples per month in parallel by the 3 methods for 1 year. IMViC tests (indole, methyl red, Voges-Proskauer, and citrate) were conducted on all gas-positive tubes. The modified A-1 test recovered higher levels of fecal coliforms than the A-1 test. Although there were seasonal and geographic variations in recovery and enumeration by the modified A-1 test, overall there was good correlation of the modified A-1 test with the EC test. Both the A-1 and modified A-1 tests were more specific for Escherichia coli than the EC test. Results of the study indicate that the 24-hr modified A-1 test can be used as an alterantive test for the standard 72-hr EC test as an adjunct to the sanitary survey for the classification and control of shellfish-growing areas waters.

Bacteriological Techniques↗
PubMedDate not supplied

Femoral capital physeal fractures in 25 foals.

The medical records of 25 horses 1 year of age or younger affected with femoral head and neck fractures during an 18 year period were reviewed. Each fracture involved the capital physis. The foals were 11 days to 12 months of age (mean, 5 months). No femoral capital physeal fractures occurred in horses older than 1 year of age during the same period. The history in each case included acute onset of severe unilateral hindlimb lameness, 3 hours to 2 months (mean, 12 days) before presentation. Injuries observed were violent falls, struggles, and kicks. Crepitation, swelling, pain with manipulation or palpation or both, and apparent fracture fragment displacement were inconsistently noted. Tentative clinical diagnoses were confirmed by radiography in 24 foals and by necropsy alone in one foal. Twenty-one foals were euthanatized due to poor prognosis. One foal sent home for stall rest was lost to follow-up. Surgical repair was attempted in three foals. Two fractures were repaired with multiple intramedullary pins and the foals were euthanatized within 2 weeks due to surgical failure and, in one case, contralateral limb breakdown. The third fracture was repaired with a compressing screw and plate device; the animal was pasture sound at month 20.

Animals↗
PubMedDate not supplied

Evaluation of an interfragmentary compression system for the repair of equine femoral capital physeal fractures.

Femoral neck and proximal epiphyseal lengths were measured in 37 femurs from 19 cadaver foals that were 1 day to 12 months old to determine the applicability of a human interfragmentary compression system to equine femoral capital physeal fractures. Because components of the implant system are available only in fixed sizes, its use was possible in foals older than 5 weeks of age, but not in younger foals. The 135 degree angle plate conformed best to the equine femur. Femoral capital physeal fractures were created surgically and repaired with the implant system in three foals. Fracture stability was evident clinically and radiographically in all three foals until euthanasia at month 3. At necropsy, the treated femurs were 4, 8, and 27 mm shorter than their mates. Epiphyseal viability was verified in all three foals by tetracycline deposition and new appositional bone growth comparable with that in the contralateral control epiphyses. The treated capital physis was open but reduced in thickness in one foal, disorganized in one foal, and closed in one foal. Fixation by compression with the implant system resulted in stability sufficient for fracture healing and maintenance of epiphyseal viability, although it was associated with reduced longitudinal femoral growth.

Animals↗