Biomedical subjects

D A Kalbhen

Publications and source records attributed to D A Kalbhen.

75 records · Page 5Linked to original sources

PubMedDate not supplied

[Histology of the antiarthritic effect of tribenoside in experimental gonarthrosis].

Animal experiments with chemically induced osteoarthrosis in the knee joint of adult hens have shown that the daily treatment with tribenoside (Glyvenol(R)) using single oral doses of 50 mg/kg or 150 mg/kg, will result in a marked reduction of the intensity and frequency of joint degenerations. This anti-arthrotic effect was quantitatively measured by radiographic and macroscopic methods over a period of 3 months. In the present study we investigated the inhibitory effect of tribenoside on osteoarthrosis by histological techniques. Histological sections of osteoarthrotic articular cartilage of untreated and treated animals were compared and clearly indicated that there were no qualitative differences in alterations of the morphology and histopathology of the tribenoside treated specimen. Only the frequency and intensity of degenerative alterations where markedly reduced in the drug treated animals. Biochemical analysis of joint cartilage demonstrated that tribenoside (150 mg/kg) significantly reduced the loss of proteoglycans in the osteoarthrotic cartilage matrix. Our histological and biochemical findings are in good correlation with the results of our radiographic and macroscopic studies on the effect of tribenoside on experimental osteoarthrosis. The present investigation indicates that tribenoside exerts its antiarthritic effect on the base of its vasotropic properties by stabilization of the capillary system and membrane of the synovial tissue, which provides the transport and exchange of nutritional substrates and metabolites via synovial fluid to or from the articular surface. Our histological findings do not exclude the possibility that tribenoside also has a direct chondroprotective effect.

Animals↗

PubMedDate not supplied

The inhibitory effects of steroidal and non-steroidal antirheumatic drugs on articular cartilage in osteoarthrosis and its counteraction by a biological GAG-peptide complex (Rumalon).

In-vitro studies have confirmed that various corticosteroids and also many nonsteroidal antirheumatic drugs exert inhibitory effects on anabolic processes in connective tissue cells. Depending on dose and at concentrations that in many cases correspond to therapeutic plasma levels, these drugs may lead to a pronounced reduction or complete blockade of synthesis of the proteoglycans and collagen of the cartilage matrix. Our in-vivo animal experiments have shown that single or repeated intraarticular applications of various antiinflammatory antirheumatics into the knee joint of hens or rats can induce progressive joint degeneration within 3-4 months. The degenerative process was monitored by macroscopic, radiological, histological, biochemical, and stereoelectronmicroscopic methods and corresponded very well with the pathology of human osteoarthrosis. Our experimental osteoarthrosis, induced by metabolic lesions or inhibition of chondrocytes, can be influenced therapeutically by intraarticular or intramuscular treatment with the biological GAG-peptide complex (Rumalon) or with its high molecular fraction DAK-16. These compounds significantly reduce or even stop the degenerative progression in the osteoarthrotic knee joints. The chondroprotective and anti-arthritic properties of Rumalon or DAK-16 can be explained by its stimulation of connective tissue anabolism as well as by its inhibitory effect on catabolic enzymes responsible for cartilage breakdown. Our experiments also indicate that catabolic reactions of corticosteroids on articular cartilage can be counteracted or reduced by concomitant administration of the chondroprotective agents. This effect might be desirable and beneficial during treatment of inflammatory phases of osteoarthrosis with intraarticular injections of corticosteroids.

Adrenal Cortex Hormones↗

PubMedDate not supplied

[Roentgenologic determination of degenerative joint diseases in a new animal experiment arthrosis model].

Our previous experiments have shown that intraarticular injections of sodium iodoacetate or phenylbutazone into the knee joint of animals (hens) induce degeneration of the articular tissues. This method proved to be a useful model for experimental osteoarthrosis. After autopsy degenerative processes in the joint can be evaluated by macroscopical, histological and biochemical techniques, and show good correlations with osteoarthrosis in humans. To study the onset and progress of experimentally induced osteoarthrosis in living animals we developed a new X-ray method using special mammography-film (Kodak Definix Medical). By this technique also changes of the joint space can be measured quantitatively. Comparison between the degenerative lesions of cartilage as seen macroscopically during autopsy and the data obtained from the living animal by X-ray techniques show excellent correlations. By using X-ray mammography film in our studies of experimental model it is possible to observe the pathological alterations of the knee joint of the living animals and to investigate the effect of pharmacologically active compounds for the treatment of osteoarthrosis.

Animals↗