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D A Keefer

Publications and source records attributed to D A Keefer.

8 recordsLinked to original sources
PubMed1978

Horseradish peroxidase as a retrogradely-transported, detailed dendritic marker.

A method is described that produces detailed 'Golgi-like' staining in neurons following retrograde transport of horseradish peroxidase (HRP). Of 4 commercially available HRP preparations, which were compared for their ability to produce solid neuronal staining in certain regions of the rabbit thalamus 48 h after striatal injection, two preparations (Miles Laboratories and Serva Biochemical) were found to be most efficient. When dimethylsulfoxide (DMSO) was used as the solvent for the injected HRP, a significant increase in the number of solidly labeled neurons in the thalamus was realized. Approximately 2% DMSO produced the greatest increase when compared with water as the solvent. Dendritic detail visualized by this method is shown to compare favorably with that produced by Golgi staining.

Animals↗
PubMed1978

Estrogen localization in the brain of the lizard, Anolis carolinensis (1).

Estrogen induces reproductive behavior in lizards by acting on specific areas of the brain. Neural areas that selectively concentrate radioactivity after 3-h-estradiol administration were mapped using thawmount autoradiography. Major accumulations of hormone-concentrating cells occur in the medial preoptic area, basal hypothalamus, amygdala (n. vetromedialis telencephali) and in and around the torus semicircularis. Numerous smaller accumulations are also described and the topographical distribution is compared with that of avian and mammalian species.

Animals↗
PubMed1975

Atlas of estrogen-concentrating cells in the central nervous system of the squirrel monkey.

Estrogen is concentrated within cellular nuclei in discrete regions of the monkey brain 30 and 60 minutes following intravenous injection of [3H] estradiol. Chromatographic data is provided to suggest that most of the localized estrogen is in the form of estradiol with lesser amounts of estrone and estriol. Three "major" areas of estrogen accumulation include: (1) preopticostrial accumulation: n. preopticus medialis--n. interstitialis striae terminalis, (2) basal hypothalamic accumulation: n. infundibularis--n. ventromedialis--n. premammillaris ventralis, and (3) the amygdaloid accumulation. Several "minor" areas of estrogen accumulation include the tuberculum olfactorium, insulae Calleja, n. triangularis septi, a. hypothalamica anterior, n. anterior hypothalami, n. paraventricularis, n. supraopticus, n. periventricularis and the substantia grisea centralis. The neocortex, rhombencephalon and spinal cord are essentially unlabeled. The major areas of accumulation are similar in several other mammalian and avian species while these, and some minor areas of accumulation, have been shown in neuroanatomical studies to be interconnected by several pathways, especially the stria terminalis. Lesion, implant, stimulation, recording and morphometric studies, in several species, support the concept that this arrangement provides a neuroanatomical substrate which would allow the integration of the various facets of the neuroendocrine reproductive response.

Amygdala↗
PubMed1975

Simultaneous autoradiographic and immunohistochemical localization of estrogen and gonadotropin in the rat pituitary.

A combined autoradiographic and immunohistochemical technique is introduced which is suitable to simultaneously localize hypophyseal sites of nuclear (3-H)-estradiol uptake and anti-hCG-characterized "gonadotropes". Most, but not all, "gonadotropes" show significant nuclear concentration of radioactivity. A large percentage of non-"gonadotropes" also show nuclear uptake of label.

Animals↗