Some quantitative investigations of the binding to and the displacement of bishydroxycoumarin from human serum albumin.
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Binding, Competitive↗
Biomedical subjects
D A Nelson
Publications and source records attributed to D A Nelson.
Zeta sedimentation ratio (ZSR), a replacement for the erythrocyte sedimentation rate (ESR).
The zeta sedimentation ratio (ZRS) was evaluated and found to have advantages over the erythrocyte sedimentation rate (ESR). The normal range for the ZSR proved to be 41 to 54%. Elevated ESR's obtained with the modified Westergren technic were also associated with an increased ZSR, except when amenia spuriously elevated the ESR. Reproducibility after storage was slightly better for the ZSR than for the ESR, But for optimal results the test should be performed within two hours of phlebotomy. Increased concentrations od EDTA anticoagulant may falsely elevate the ZSR, especially if the ZSR is initially elevated.
Adult↗
Predictive value of in vitro colony and cluster formation in acute nonlymphocytic leukemia.
Granulocytic-monocytic colony-forming activity of marrow cells was evaluated at presentation in 20 patients with acute nonlymphocytic leukemia (ANLL). A double-layer agar culture was employed to determine colony- and cluster-forming activity. All patients underwent induction chemotherapy with cytosine arabinoside and daunorubicin. Five patients died during induction. Eight of the 11 patients entering complete remission had absent or decreased colony and cluster growth of marrow cells. Four patients who failed to obtain a complete remission produced increased or only cluster growth in culture. Our data support the conclusions of others that decreased colony and cluster formation in vitro is associated with a more favorable response to induction chemotherapy for ANLL.
Acute Disease↗
Re-examination of the effects of periodic and aperiodic stimulation on the auditory-evoked vertex response.
It has been hypothesized that aperiodic stimulation will produce larger auditory-evoked vertex responses than periodic stimulation. Attentiveness toward an acoustic stimulus by human subjects has been shown in the past to enhance the auditory-evoked response. The unpredictability of aperiodic intersignal intervals has been assumed by previous investigators to produce more attentive listening behaviour and has therefore been used clinically to obtain 'enhanced' evoked responses. In order to examine this question further, auditory-evoked vertex responses were obtained with variable-interval (aperiodic) and with fixed-interval (periodic) schedules of stimulus presentation that had geometric mean intersignal intervals of 5 or 10 s. The evoked responses were obtained from normally-hearing adults while they were engaged in a counting task and while they were reading. Tone bursts were presented at 30 and 60 dB sensation level. No significant differences in peak-to-peak amplitude measures of components with latencies shorter than 300 ms could be attributed to aperiodic stimulation. When the time-base on the signal averager was extended to include components with latencies longer than 300 ms, no significant changes in component amplitudes could be attibuted to aperiodic stimulation, even though peak-to peak amplitudes during an attentive state (counting) were nearly double those obtained during an inattentive state (reading). It was concluded that aperiodicity in intersignal intervals is not a useful technique to employ in order to produce larger and more identifiable evoked responses for clinical diagnosis.
Acoustic Stimulation↗
Comparison of platelet glass bead retention techniques in patients with clinical bleeding disorders.
Platelet glass bead retention by the Salzman and infusion pump techniques were compared in 36 control individuals, 10 patients with von Willebrand's syndrome and four patients with thrombocytopathies. No significant differences between the results of the two assays were observed. Eleven percent or less of the control individuals had diminished platelet glass bead retention. The mean percentage platelet retention of the von Willebrand's group and the thrombocytopathy group was less than the mean platelet retention of the control group. However, at least 50 percent of the patients with von Willebrand's syndrome had normal platelet retention by both methods. In the thrombocytopathy group, glass bead retention was more consistently diminished. Although an inverse correlation existed between the duration of bleeding time and the platelet retention assays, consistently diminished platelet retention results were observed only when the bleeding time was 18 minutes or longer. It has been concluded by us that while the glass bead retention assays may be helpful in characterizing certain types of platelet disorders, they are not useful screening assays in the diagnosis of von Willebrand's syndrome.
Blood Coagulation Disorders↗