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Biomedical subjects

D Amels

Publications and source records attributed to D Amels.

5 recordsLinked to original sources
PubMedDate not supplied

Contributions to the study of bisazo dye(s) induced eye anomalies in rats.

Eye anomalies were studied in embryos and foetuses of pregnant Wistar albino rats injected i.p. on day 9 of pregnancy with trypan blue (8-15 mg/100 g) and Niagara sky blue 6B (10-15 mg/100 g). Specimens were obtained by killing or by repeated surgical interventions between the 12th and 20th day of pregnancy. Microscopical changes were recorded in 170 embryos and foetuses of the experimental series and in 50 control specimens. Anophthalmia nad microthalmia (of various degrees) were the main anomalies induced by both dyes used. Other anomalies, less frequent, involved the whole eye or one or more eye components. No degenerative and necrotic changes the whole eye or one or more eye components. No degenerative and necrotic changes were recorded and no features attesting the vascular origin of malformations could be found. The persistence of eye appendages even in the total absence of any eye rudiment was constantly observed. Control specimens showed no microscopical changes of the developing eye. Some problems concerning possible pathogenic pathways are discussed.

Abnormalities, Drug-Induced↗
PubMedDate not supplied

Contributions to the transfer of preimplantation molse embryos into "foster-mothers".

A new method for the transfer of preimplantation stages in mice has been developed by using as recipient the "pregnant empty uterus" obtained by ligature at the utero-oviductal junction. Results obtained with several combinations between Wh, CBAT6T6, C57Bl6 strains varied according to transfer media and strain combination, the best percentage of taking being under the level of those reported by other authors. By using transfer within the RAP strain, taking (controlled at 14 days of pregnancy) was similar to the best results generally obtained by the surgical transfer method. The advantages offered by the transfer method are briefly discussed.

Animals↗
PubMedDate not supplied

6-Aminonicotinamide-induced eye defects in rats.

The pathological changes and structural anomalies induced by 6-aminonicotinamide (6-AN) in the developing eye were studied in rats (Wistar and hooded randombred strain). The substance was administered in aqueous solution intraperitoneally (4 mg/kg on day 9--10 of pregnancy: 5 mg/kg on day 11 of pregnancy; 8 mg/kg on day 13--17 of pregnancy) and in physiological saline intraamniotically (0.01 ml of a 1% solution in physiological saline on day 15 of pregnancy). Embryos and foetuses from experimental series and from untreated control series were macro- and microscopically examined on day 10--20 of pregnancy. Control foetuses from mothers injected with distilled water on day 9--17 of pregnancy were examined on day 20 of pregnancy. The pathological changes and structural anomalies detected at successive developmental stages are presented. They reveal an obvious phase specificity and attest that the same substance may act through both of the main teratogenic pathways hypothetically put forward by Menkes et al. (1970). Based upon the present findings (and some previous results obtained in experiments with bisazo dyes) a working hypothesis is tentatively presented, as to the possible determination of the uni- or/and bilateral distribution of chemically induced developmental defects. In connection with some reversible or transitory pathological changes the role of recovery in teratogenesis is pointed out.

6-Aminonicotinamide↗