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D Anaise

Publications and source records attributed to D Anaise.

69 records · Page 4Linked to original sources
PubMed1984

Enhanced resistance to the effects of hypothermic ischemia in the preserved canine kidney.

Addition of trifluoperazine (TFP), a powerful calmodulin inhibitor to Collins' flush solution has exerted a significant protective effect on the cold-preserved kidney, with successful autotransplantation of 80% of preserved kidneys (4 of 5) after 72 hr of storage. In contrast, use of Collins' solution alone resulted in successful autotransplantation of only 33% (2 of 6) of kidneys after a similar period of preservation. In an attempt to analyze the significance of this result, the microcirculation of preserved kidneys was studied with injections of technetium-labeled microspheres into the kidneys, followed by study with a noninvasive radionuclide scintiphotography (RNS) technique that does not interfere with subsequent transplantation of the kidney. Such studies demonstrate that prolonged cold preservation after flush-cooling with Collins' solution is associated with a progressive deterioration of the integrity of the microcirculation, resulting in poor flow to the renal cortex. In contrast, when TFP is added to the Collins' solution, there are uniform increases in renal cortical flow in kidneys stored for 48 and 72 hr, with preservation of the integrity of the renal microcirculation. Biological testing shows a clear-cut correlation between these observations and the capacity of the tested kidneys to sustain life after retransplantation. Further experiments suggest that the decreases observed in cortical flow in kidneys preserved in the cold for 72 hr are a consequence of cellular swelling, and not of a vasospastic response. The data support the conclusion that TFP exerts its protective effect on the basis of its membrane stabilizing capacity as a calmodulin inhibitor, and not through direct vasodilatation.

Animals↗
PubMed1984

Scintigraphic evaluation of the viability of cold-preserved kidneys before transplantation.

Renal microcirculation studies utilizing microspheres can provide a highly reliable index of the viability of preserved kidneys. We describe a noninvasive method that permits subsequent transplantation of each tested kidney. Fresh canine kidneys were removed and perfused with either Collins' solution or Collins' solution with 5 mg/l of trifluoperazine (TFP), and preserved at 4 degrees to 6 degrees C in their respective perfusates for 5, 24, 48, or 72 hr. At the end of each period, the preserved kidney was perfused with 50,000 Tc-99m-labeled microspheres. Uniform cortical activity occurred in all kidneys preserved for 5 hr, with moderate decreases in cortical activity at 24 hr. Kidneys perfused with Collins' solution showed progressively decreasing cortical flow with sparse cortical activity after 72 hr. In kidneys perfused with Collins' solution containing TFP, however, cortical flow remained relatively well preserved at 48 and 72 hr. Direct correlation between viability of the cortical microcirculation and the capacity of the corresponding kidney to sustain life upon retransplantation into the original host was observed.

Animals↗
PubMed1982

Treatment of cutaneous malignant melanoma in Israel, 1960 to 1970.

A retrospective study was performed of 512 patients suffering from primary cutaneous malignant melanoma of the skin who had been treated in Israel between 1960 and 1970. The patients were randomly admitted to six hospitals and treated according to different protocols, based on the policy of each hospital. Our conclusions are that in stage I of the disease, radical prophylactic lymph-node dissection (RPLND) improved the prognosis in all three levels of tumor invasion into the skin; in stage II of the disease, RPLND did not improve the prognosis when compared to wide excision of the tumor and excision of the clinically involved nodes; and in stage I of the disease, there is no statistically significant difference in prognosis between excisional biopsy and wide excision of the tumor.

Female↗