Biomedical subjects

D Arenholt-Bindslev

Publications and source records attributed to D Arenholt-Bindslev.

23 records · Page 2Linked to original sources

PubMed1987

The growth and structure of human oral keratinocytes in culture.

Human keratinocytes derived from explants of cheek (buccal) mucosa grow vigorously in culture and can be subcultivated twice. The structure of the oral keratinocytes in vitro is the same in primary cultures and subcultures. The cells stratify, are characterized by well-developed tonofibrillar-desmosomal complexes, and rarely exhibit signs of terminal differentiation. Unique features of the culture system that favor keratinocyte growth are: incubation at 34 degrees C, inclusion of 0.5% dimethyl sulfoxide in the culture medium, and initiating subcultures as 5.0 mm colonies containing 100,000/20 microliter of medium. One primary culture can yield 6 first-passage subcultures, which subsequently achieve confluence in 10-12 days. Such cultures are a useful source of human keratinocytes that stratify but generally do not undergo terminal differentiation.

Cell Division↗

PubMed1987

Angiogenic activity of malignant oral epithelial cells: a preliminary study.

We investigated the angiogenetic capacity of normal and malignant oral epithelial cells by intradermal injection of cells into nude mice. Cells investigated: 1) a tumorigenic cell line from rat palate; 2) a non-tumorigenic epithelial cell line from rat palate; 3) cells from second passage of rat palate epithelium explant cultures; 4) cells from epidermis of neonatal mice. A 0.1 ml suspension containing 10(5) epithelial cells was injected into the flank region. Mice were killed 8 days later to evaluate angiogenesis. Under a dissection microscope, the number of vessels in the skin adjacent to the injection site was counted. The tumorigenic cell line demonstrated the strongest angiogenetic capacity and the non-tumorigenic cell line was more angiogenic than any of the other non-tumorigenic cell types. The greater angiogenic activity of neoplastic oral epithelial cells may be a useful feature in the evaluation of oral malignant development.

Animals↗

PubMed1987

Toxic effects of two dental materials on human buccal epithelium in vitro and monkey buccal mucosa in vivo.

Confluent cultures of human buccal epithelial cells were exposed to graded dilutions of Gluma Bond or 3M Etching Liquid for 5 min. The cytotoxic effects induced by this treatment were observed (epithelial cell damage, growth inhibition). In vivo, monkey buccal mucosa was exposed to Gluma Bond or 3M Etching Liquid for 5 min. Biopsies were taken after 24 h, and the buccal epithelium processed for light microscopic examination. The toxic reactions to Gluma Bond were far more pronounced compared with the toxic reactions to 3M Etching Liquid in both models. Data obtained suggest that the in vitro model may be useful in assessing mucosal toxicity and in studying mechanisms of toxic action.

Aldehydes↗

PubMedDate not supplied

Antagonistic effect of selenite on tumor promoter induced cell proliferation in cultures of rat tongue epithelium.

In cultures of rat tongue epithelial cells, cell proliferation following incubation with different doses of the potent tumor promoter TPA has been studied by using a stathmokinetic method counting colchicine arrested metaphases. It was demonstrated that 24 h incubation with concentrations higher than 5 ng TPA/mL medium caused inhibition, whereas below 5 ng TPA/mL medium caused stimulation of the mitotic activity reaching a maximum around 30 h from the start of the incubation period. Based on the evidence of the anticarcinogenic effect of selenium in several animal models, experiments have been performed elucidating the influence of an atoxic dose (1/1.000.000M) of selenite on the observed TPA-induced cell proliferation. Our results indicate that addition to the culture medium of an atoxic dose of selenite, not affecting the mitotic activity of control cultures, inhibits the TPA-induced stimulation of cell proliferation.

Animals↗

PubMedDate not supplied

Effect of folic acid on human oral epithelium in vitro.

The cytomorphological effects of folic acid were studied using in vitro established human oral epithelium. It was demonstrated that a concentration twice that used clinically (200 micrograms/ml) did not induce marked cytotoxic reaction in the cultured cells. The most pronounced changes were observed in cultures exposed to 200 micrograms/ml folic acid both in primary culture and subculture. The cultures displayed areas of degenerating cells showing oedema and increased translucency of the cytoplasm, flattened cells with distinct tonofilaments and atypical mitotic figures. Identical changes were found in cultures exposed to 50 and 100 micrograms/ml folic acid but to a lesser extent than in 200 micrograms/ml. These changes indicated that folic acid increased the number of cells undergoing terminal differentiation. From this study we suggested that folic acid when applied topically may play a role in local stimulation of epithelial cell differentiation leading to enhanced healing of oral ulcers.

Cells, Cultured↗