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D Arndt

Publications and source records attributed to D Arndt.

At least 73 records · Page 4Linked to original sources
PubMed1976

The peripheral kinetics of human radiolabelled eosinophils.

Peripheral kinetics of eosinophils were studied in three subjects with normal hematological conditions after a puls labelling with 0.1 mu Ci 3HTdR per g bodyweight. The computer recorded labelling index curves (LI) presented an undulating course in all three subjects. Eosinophils proved to disappear randomly. The emergence time of 13 h was followed by the first LI peak of 17%. 80 h p.i. a massive immigration of highly labelled cells with a LI value of 58% was encountered. 50% of eosinophils remained labelled up to 11th day p.i. Cells with different labelling intensities arrayed to grain groups presented a clear synchronism of their LI-curves which become less clear with time. The mean t/2-value for all labelling intensities amounted to 8 h. Cells with low labelling intensities had a longer and the highly labelled eosinophils a much shorter t/2-values. These effects suggest the existence of two proliferating eosinophil precursor pools: a rapid and a slow one. The early influx of low labelled as well as the late emergence of great number of highly labelled eosinophils are in good accord with this assumption. The time span for S-phase indirectly calculated revealed 13 h. The myelocyte generation time was estimated with 34 h. Both values were found to well agree with the corresponding data for other granulocytes as reported on, in the literature.

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PubMed1975

[5-Aminoacridine hydrochloride for fixation of human blood basophils (author's transl)].

5-aminoacridine hydrochloride (0.4 percent in 50 percent aqueous ethanol) proved to be a useful fixative for the preservation of human blood basophils pretreated with methanol-formaldehyde (9:1 V/V). Leaching of the water-soluble basophil granules or other artificial cell alterations common to fixatives in current use were not observed. The organic compound used did not induce any chemographic artifacts in nuclear tracers, in contrast to most of the other fixatives hitherto applied. 5-aminoacridine hydrochloride is therefore particularly suitable for autoradiography of the human blood basophils.

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PubMed1975

An autoradiographic study of human blood basophils.

The aim of this paper is the selective visualization of human blood basophils in autoradiographs. [(3)H]Thymidine-labelled basophils in buffy coat smears were fixed in methanol-formaldehyde follwed by 5-aminoacridine hydrochloride and stained with basic Aldehyde Fuchsin prior to autoradiographic processing. The technique described represents a simple method for quantitative autoradiographic studies on basophil kinetics and the interaction of these cells with IgE-mediated atopias.

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PubMed1975

[Autoradiographic demonstration of human basophils].

A new technique is described which enables a selective tinctorial visualization of human blood basophils in autoradiographic preparations. Consecutive administration of 5-aminoacridine hydrochloride and aldehyde fuchsin rendered a sufficient preservation of basophils preventing leaching of their water soluble granules. Extensive control tests provided sufficient evidence to exclude chemographic artifacts produced by other histochemical precedures. The technique eliminates the obstacles to study the kinetics of human blood basophils and their realtionshp to atopic hypersensitivity reactions applying tritiated thymidine and radioiodine labeled IgE or corresponding antisera.

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PubMed1975

[Fatal skin epithelioma in a stomatologist professionally exposed to x-rays].

An epithelioma of the volar face of the right index caused the death of a stomatologist whose skin had been exposed to X-rays for many decades when keeping dental films in place. The casuistics of the clinical picture are dealt with, and arguments are given for its recognition as an occupational disease. The evaluated X-rays dose (the accumulated dose was approx. 2,100 rem, i.e. relatively low) and modern knowledge on radio-medicine are commented with regard to dose-effect relationship.

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PubMedDate not supplied

Liposomal bleomycin: increased therapeutic activity and decreased pulmonary toxicity in mice.

Conventional and sterically stabilized liposomes derived from phosphatidylcholine or the antitumor agents, hexadecylphosphocholine and octadecyl-(1,1-dimethyl-4-piperidino-4-yl)-phosphate, as bilayer forming constituents, containing bleomycin, were developed and tested. Liposomal encapsulation of bleomycin enhanced strongly the antitumor activity against P388 leukemia and the Lewis lung carcinoma. This effect was clearly dependent on the size and lipid composition of the bleomycin-containing liposomes. The therapeutic effects were nearly equal for liposomal and free bleomycin in the B16 melanoma. The partial replacement of phosphatidylcholine by alkylphospholipids and the inclusion of polyethylene glycol modified lipids for sterical stabilization did not further improve the therapeutic efficacy but increased, in some cases, the toxicity of liposomes. Bleomycin-induced lung injury was not observed if liposomal bleomycin was administered.

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