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D Arnoux

Publications and source records attributed to D Arnoux.

42 records · Page 3Linked to original sources
PubMed1980

[Comparative study of normal and psoriatic skin using a micromethod for detection of enzymatic activity].

We used a semiquantitative micromethod for detection of enzymatic activity (APIZYM) which allows a fast and simultaneous study of 67 hydrolase and dehydrogenase activities on dermo-epidermic skin samples. This wide investigated field led to the determination of the enzymatic profile (called zymogram) of the samples under study. This method was applied to normal skin and to psoriatic and uninvolved psoriatic skin; our results exhibited the good reproductibility of the method and the homogeneity of the studied samples. The enzymatic activities of the psoriatic skin are, in mean, twice those of normal skin. The uninvolved psoriatic skin showed reactions close to those of the normal skin. These observed variations of those different activities are perfectly similar to literature datas. The APIZYM method, thanks to its easy utilization and its fiability seems extremely interesting in the study of zymogram of cutaneous samples. The use of APIZYM in observations on naevus and malignant melanoma is to be exposed in a following publication.

Adult↗
PubMed1980

[A case of disseminated histoplasmosis due to Histoplasma capsulatum (author's transl)].

A case is reported of American histoplasmosis due to Histoplasma capsulatum presenting as a hepatic granulomatosis. The patient was a young adult from Haiti and investigations revealed the presence of an ulcerous enterocolitic suggesting tuberculosis or Crohn's disease. The condition became worse after antituberculous therapy and corticoids, and operation was necessary. Extemporaneous histological examination, of the colonic ulcerations showed Histoplasma capsultaum yeasts. The patient, who had a cellular immunity deficiency, died during antifungal treatment with amphotericin B of the disseminated histoplasmosis is rare: 1 case in 5000 American histoplasmosis cases. Digestive tract lesions, usually detected during operation or autopsy, are found in about 20% of cases. In the present observations the diagnosis of American histoplasmosis was based on the country of origin of the patient, but this was not confirmed: the immunological reactions were and remained negative and no yeasts were demonstrated after staining of pre-operative biopsy specimens (colon and liver particularly). Also, serial sections conducted after the diagnosis had been established demonstrated the presence of Histoplasma capsulatum in the colon sample removed during colonoscopy.

Adult↗
PubMedDate not supplied

[Antiphospholipid antibodies: clinical significance and biological diagnosis].

The term "antiphospholipids" (aPLs) refers to an heterogeneous family of antibodies diagnosed either by clotting tests: the lupus anticoagulants or by Elisa: anticardiolipin (aCL) and anti-beta2-glycoprotein I (anti-beta2GP1) especially. aPLS recognize phospholipids, alone or bound to plasma protein cofactor(s), or the cofactors themselves. aPLs have long been described in autoimmune diseases such as SLE, but may also be found in other clinical settings including infections, malignancies and drug administration. Their persistent presence can be associated with venous and/or arterial thrombotic complications and/or recurrent miscarriage, thus defining the "antiphospholipid syndrome" (APS). The heterogeneity of aPLs makes a comprehensive approach to laboratory investigation essential. Detection of lupus anticoagulants relies on increased clotting times in phospholipid-dependent tests. Their 4 step diagnosis includes: 1) screening (by at least two different tests); 2) demonstration of an inhibitory activity; 3) evidence of its phospholipid dependence; 4) exclusion of an associated coagulopathy. Among the aPLs detected by Elisa, IgG aCL are the most frequently investigated. However, other antibodies may represent useful biological tools. Among them, anti-beta2GP1 are thought to be more closely associated with a history of thrombosis than aCL and testing for anti-beta2 GP1 should now be systematically included in the biological diagnosis of APS. The Elisa used for aCL and anti-beta2GP1 are not fully standardized, and a number of methodological parameters may account for the interlaboratory discrepancies often observed. The clinical importance of other antibodies such as antiphosphatidylethanolamine, antiprothrombin or antiannexin V is being evaluated. An appropriate laboratory investigation of APS should, in all cases, combine the use of clotting and immunological assays, and assess the persistence of autoantibodies over time.

Adult↗