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D B Alymbaeva

Publications and source records attributed to D B Alymbaeva.

7 recordsLinked to original sources
PubMed1995

[Morphological changes in the gastric mucosa in workers with chronic mercury poisoning].

The authors studied morphologic changes in gastric lining of patients facing chronic mercuric intoxication. The examinees are former burners at metallurgic plant of Khaidarkansk mercuric enterprise. The patients demonstrated significant destruction of the superficial epithelial cells and the accumulation of Campylobacter piloridis. The parietal and zymogen cells appeared to have depressed functional activities. The cells in gastric antrum had ultrastructure characterized by vacuoles with dense filaments which could be a morphologic marker of mercuric intoxication.

Aged↗
PubMedDate not supplied

[Experimental hepatitis E infection in piglets].

A sample of patient's faeces containing virus-like particles (VLP) of 27-34 nm was obtained during an outbreak of hepatitis E in the Kirghiz SSR. The identity of the VLP to hepatitis E virus and etiological association with the disease were demonstrated by immune electron microscopy and infection of Macaca fascicularis monkeys. This isolate of hepatitis E virus is able to induce experimental infection in domestic piglets which was very similar to experimental hepatitis E in primates when infected orally, intravenously and by the combined routes. The clinical manifestations included acute biochemical and histological hepatitis, excretion of hepatitis E virus in faeces, icteric sclerae and skin, hepatitis virus presence in the material from mesenteric lymph nodes. Immunosuppression aggravated hepatitis E infection in piglets. Piglet-to-piglet transmission of hepatitis E virus was demonstrated. During passages of the virus in piglets a shortening of the incubation period and the absence of jaundice was observed.

Animals↗
PubMedDate not supplied

[Characteristics of electrophoretic types of rotaviruses isolated from children with acute gastroenteritis].

In an analysis of sporadic cases of gastroenteritis in children, rotaviruses were detected in 61 out of 363 specimens examined. Studies of rotavirus isolates by polyacrylamide gel electrophoresis (EP) of RNA revealed 9 different EP types. In one region, during the entire epidemic season one "long" EP type was prevalent. In one patient, a mixture of a "short" and "long" EP types was detected. In another region, "atypical" rotaviruses were found not belonging to the antigenic group A of rotaviruses.

Acute Disease↗
PubMedDate not supplied

[Influence of mycoplasma on the production of the oncogenic leukemia virus HTLV by HUT-102 T-lymphoblastoid cells].

A suspension culture of human T-lymphoblasts HUT-102 producing type C oncovirus (HTLV) was examined electron microscopically. Control decontaminated HUT-102 cells spontaneously contaminated with M. orale and exogenously infected with M. pneumoniae were examined in ultrathin sections. The variation statistics method demonstrated that in the mycoplasma-infected cells the number of HTLV virions was significantly higher than in control cultures. It is assumed that the increased production of HTLV is due to mycoplasma-induced changes of physico-chemical properties of the cell membrane which facilitate virion budding.

Cell Line↗
PubMedDate not supplied

[Further study of experimental hepatitis E in piglets].

Experimental infection of piglets with a fecal pool containing hepatitis E virus (HEV) strains Osh-25 and Osh-228 confirmed previous results obtained with HEV strain Osh-205 on susceptibility of these animals to hepatitis E. A biphasic increase of transaminase activities, histopathologic changes in the liver, virus excretion with feces were observed in the animals. The genome of viral particles in piglet feces conformed, at least partially, to human HEV RNA. Ultrastructural abnormalities of piglet hepatocytes resembled those in primates with experimental hepatitis E. The number of positive results (during follow-up of the particles and HEV RNA in various organs of experimental animals) was the highest on day 10 after infection. Both the particles and HEV RNA were present in the lymphoid tissue over the entire follow-up period, from day 5 to day 15 postinfection.

Animals↗
PubMedDate not supplied

[An experimental infection in lambs by the hepatitis E virus].

Lambs were experimentally infected with a pool of 10% hepatitis E (HE) patient fecal suspension containing HE virus (HEV) isolates Osh-225 and Osh-228 which caused an infection closely resembling experimental HE in primates. Clinical manifestations consisted of acute biochemical and histological hepatitis, virus shedding in feces, the presence of virus-like particles in the peripharyngeal lymph nodes and the contents of small intestine, of HEV RNA in the parenchymal organs of lambs. Susceptibility of lambs to HEV derived from the infected piglets and the possibility of passaging the piglets' HEV in lambs was demonstrated. This passaging led to shortening of the incubation period.

Animals↗