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Biomedical subjects

D B Krivulis

Publications and source records attributed to D B Krivulis.

10 recordsLinked to original sources
PubMed1978

[Effect of hypothermia on metabolism in the liver during its preservation].

Twenty experiments were conducted on dogs. The effect of hypothermia of different degree (from 18 to 20 degrees C and from 4 to 6 degrees C) on the carbohydrate metabolism and the extent of solubilization of hepatic enzymes (lactate dehydrogenase, glutamate dehydrogenase, urokaninase, DNA-ase, glucose-6-phosphatase) in prefusion-free preservation of the liver was studied. The preservation efficacy was assessed during the subsequent two-hour normothermic perfusion. A marked solubilization of the enzymes under study followed preservation of the liver at 18--20 degrees C; this indicated the loss of intactness of the cell membranes during the preservation. A moderate expenditure of the glycogen stores in the liver, and of sugar in the perfusate followed preservation of the liver at a temperature of 4--6 degrees C; this suggested an even suppression of hepatic metabolism and the prevalence of normal tissue respiration over glycolysis in the restoration of circulation in the liver.

Animals↗
PubMed1976

[Absorptive-excretory function of isolated dog liver during its perfusion].

Twenty experiments were performed on mongrel dogs. The absorptive and excretory function of the liver in the intact organism and during its isolated perfusion through the portal vein with the use of extracorporeal circulation was studied by means of the bromsulfaline test. The absorptive function of the perfused liver proved to decrease by 50-60 per cent. The rate of bile elimination and plasma clearance was 5-6 times higher at the beginning of the experiment in the intact organism. Later on, the difference in the degree of retention of the hepatic excretory function was reduced. Hypoxia connected with denervation, hepatectomy and the isolated perfusion proper apparently served as the principal cause of reduction of the absorptive-excretory function of the isolated liver.

Animals↗
PubMed1976

[Solution for liver preservation].

The author suggested a solution for preservation of the liver. The specific feature of the solution is that it contains special substances of carbohydrate and protein nature which, requiring no complicated metabolic transformations, can easily be involved in the liver metabolism during the period of its preservation. Observations have demonstrated that the preserving substance suggested by the author is much better than analogous solutions employed in control experiments.

Animals↗
PubMed1975

[The function of the isolated heterologous liver perfused with the human blood (author's transl)].

The metabolic changes and their influence on the isolated liver were studied in 21 experiments on pig liver, perfused with human blood during the period of 3 hrs. It was detected, that rather active carbohydrate and nitrogenic metabolism has been maintained in the liver during 1.5-2 hrs. But after the 2nd hr of perfusion the phenomens of perfusion the phenomens of hypoxia increased, that caused the inhibition of metabolic processes and leaded to deterioration of liver function. The experience of clinical perfusions showed, that the functional activity of assisted liver over the period of 90 min was so depressed, that the organ couldn't provide positive clinical effect.

Animals↗
PubMed1975

Aspects of anaesthetic management of heterologous extracorporeal hepatic support in patients with acute liver failure.

Some anaesthetic aspects of heterologous extracorporeal hepatic support for patients with acute liver failure have been investigated in animals and men. Two types of anaesthesia during hepatectomy in pigs were performed in 20 experiments divided into two groups. A convenient anaesthetic technique was found to be the combined use of neuroleptanalgesics, gamma-OH, small doses of thiopental sodium and nitrous oxide--oxygen mixture. The necessity for care in the procedure of temporary liver support for 'poor-risk' patients is outlined. A minimal use of drugs which are metabolized by the liver, and avoidance of potent analgesics, narcotics, hypotension and hypoxia are the main principles for safe extracorporeal hepatic assistance to critically ill patients.

Adult↗
PubMedDate not supplied

Intensive therapy for hepatic coma.

Of 45 patients observed in the ICU with severe acute hepatic insufficiency, 15 patients were in hepatic coma. All patients received combined treatment consisting of standard conservative methods (drug therapy) and surgical methods for temporary support of liver function (hemodialysis, exchange blood transfusion, pig liver perfusion). Intensive therapy which began during the early phase of hepatic coma enabled us to bring six patients out of the coma, four of whom completely recovered. These studies showed that of the diseases causing hepatic coma, the worst results were obtained with viral hepatitis. This may be explained both by the extensive liver damage and marked metabolic disturbances, which led to failure of other vital organs and systems. At present, the combined therapy contributes to a greater percentage of recovery of patients with hepatic failure and coma.

Adrenal Cortex Hormones↗
PubMedDate not supplied

[A possible complication of therapeutic plasmapheresis].

Possibility of citric intoxication as a complication of therapeutic plasmapheresis is being discussed on the basis of two clinical observations. Hemodynamic reactions to sodium citrate administration have been noted during reinfusion of auto red cell mass. It is suggested that the development of collapsoid reaction, accompanied by a significant decrease in the total peripheral resistance at a stable cardiac output and cardiac index, is determined by the transition of the ionized calcium of the blood into calcium citrate, and thus the violation of the processes regulating the excitement and constriction of the muscular cells in the peripheral arteries. On the basis of further observations (360 therapeutic plasmapheresis procedures) gradual injections of small calcium quantities are recommended for the prevention of citric reactions during reinfusion of auto red cell mass.

Adult↗
PubMedDate not supplied

[Treatment of terminal forms of icterohemorrhagic leptospirosis].

Complex therapy, using hemosorption in combination with hemodialysis and hyperbaric oxygenation, was suggested for the treatment of patients with terminal stages of icterohemorrhagic leptospirosis. Such complex therapy decreased the mortality rate of patients with terminal stages of icterohemorrhagic leptospirosis from 76-96% to 18-22% and reduced the in-hospital stay from 60-80 to 35-40 days.

Hemoperfusion↗