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Biomedical subjects

D B Morton

Publications and source records attributed to D B Morton.

At least 91 records · Page 5Linked to original sources
PubMed1984

Pharmacology of the octopamine-stimulated adenylate cyclase of the locust and tick CNS.

The presence of an octopamine-stimulated adenylate cyclase is shown in homogenates of the cerebral ganglion of the locust Schistocerca americana gregaria and the synganglion of the tick Boophilus microplus. A detailed study of the pharmacology of the activation of the enzyme of the two species is reported and compared with the pharmacology of octopamine receptors in other invertebrate preparations.

Adenylyl Cyclase Inhibitors↗
PubMed1984

Structural alterations to Proteus mirabilis as a result of exposure to haemolymph from the larvae of Galleria mellonella.

Scanning and transmission electron microscopy was used to examine Proteus mirabilis, strain 11-1A, for morphologic alterations following exposure to normal and immune cell-free haemolymph obtained from Galleria mellonella larvae. It was found that both normal and immune haemolymph exerted effects on the bacterial cells, with immune haemolymph affecting a much higher percentage of cells than normal haemolymph. The morphological damage observed by scanning electron microscopy (SEM) included abnormal shapes, blebbing of the cell wall, surface erosion and lysis of the cell. Transmission electron microscopy showed evidence of damage to the cell wall and supported the results obtained from SEM.

Animals↗
PubMed1979

The effect on fertility of vaccinating female sheep with biochemically defined fractions of ram spermatozoa.

Sixty sheep were vaccinated with six biochemically defined fractions of ram spermatozoa obtained by differential extraction procedures with 0.1% Triton X-100 and 2 M MgCl2, followed by ion-exchange chromatography. No one group showed a significant reduction in fertility as compared with the controls, but there was some evidence that MgCl2 extracts were not more potent infertility-inducing agents. Some of the mechanisms by which the immune response could influence fertility are also discussed.

Animals↗
PubMed1978

The immune response of the genital tract of the female rabbit following systemic and local immunization.

Female rabbits were immunized with a model antigen, horseradish peroxidase (HRP), either locally in the reproductive tract or systemically via the intramuscular route. A sensitive immunoassay was developed to detect antibodies to the HRP in both serum and reproductive tract flushings. Systemic immunization resulted in circulating and reproductive tract antibodies of the IgG class, but none of the IgA class. Local immunization produced IgA and IgG antibodies in the reproductive tract, and also IgG antibodies in serum. Cell-mediated immunity was examined by circulating lymphocyte transformation and skin sensitivity to HRP. Cell-mediated immune responses occurred after systemic immunization, but not after local immunization. The relevance of the results to immunological methods of fertility control is discussed.

Animals↗
PubMedDate not supplied

The effect on fertility of immunizing female sheep with ram sperm acrosin and hyaluronidase.

Female sheep were injected with highly purified and partially purified preparations of ram sperm acrosin and hyaluronidase. The fertility and immune response of the sheep were monitored. Fertility was not significantly reduced in any single group, though a positive correlation was observed between high antibody titres against acrosin and reduced fertility. Studies on the direct action of sera from the ewes on ejaculated ram spermatozoa did not show any evidence of sperm agglutination or immobilization. Similar studies with denuded spermatozoa (detergent induced 'acrosome reaction') sometimes resulted in agglutination and enzyme inhibition was also seen; there was no correlation between any of these parameters and pregnancy.

Acrosin↗