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Biomedical subjects

D B Wheldon

Publications and source records attributed to D B Wheldon.

12 recordsLinked to original sources

A simple and safe volumetric alternative to the method of Miles, Misra and Irwin for counting viable bacteria.

A rapid and simple method for counting viable bacteria is described. The technique involves the use of a semi-automatic micropipette and disposable glass-capillary tubes. Viable counts obtained by this method were not significantly different from those obtained by the Miles, Misra and Irwin method or by a pour-plate method. The micropipette procedure is less hazardous than techniques involving the use of calibrated dropping pipettes.

Bacteriological Techniques↗

Multiplication of contaminant bacteria in urine and interpretation of delayed culture.

A prospective study of the bacterial populations of non-infected urine was mounted in an attempt to define the length of delay between voiding and analysis during which culture would not give false positive results due to the multiplication of contaminant bacteria present at the time of voiding. The findings suggest that culture of urine within four hours of voiding is likely to give a true indication of the presence or absence of infection. With further delay the interpretation of a heavy growth of bacteria in urine becomes progressively more unreliable, even if that growth is in pure culture.

Bacteria↗