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D Babić

Publications and source records attributed to D Babić.

At least 37 records · Page 2Linked to original sources
PubMed1997

Glutathione S-transferase activity as an early marker for malignant tumors of corpus uteri.

Glutathione (GSH) concentrations and glutathione S-transferase (GST) activities were determined in 30 paired malignant human corpus uteri tumor samples and in samples of adjacent normal tissues. For GSH concentrations no difference was found between normal (126.0 +/- 52.4 nmol/mg protein) and tumor tissues (110.1 +/- 46.4 nmol/min/mg protein; p = 0.219). The GST activities were significantly higher in tumor tissues (322.4 +/- 135.54 nmol/min/mg protein) than in corresponding normal tissues (224.6 +/- 95.64 nmol/min/mg protein; p = 0.005). This activities were independent on the pathohistological and clinical factors, except for positive lymphovascular invasion and myometrial invasion (over 50%), where significantly lower GST activities were found. For normal tissues the positive correlation between GSH concentrations and GST activities was found (correlation coefficient = 0.50, p = 0.005), but not for tumor tissue (correlation coefficient = 0.20, p = 0.281). The prognosis of patients (according to the well established prognostic factors, such as tumor type, myometrial invasion and grades) who had lower GSH concentration and GST activity in normal tissue was similar to those with higher GSH concentration and GST activity. In conclusion, higher GST activities found in tumor of corpus uteri suggest, that GST activity could be used as a tumor marker for the early stages of these malignant tumors.

Adult↗
PubMed1996

Prognostic parameters for survival of patients with malignant mesenchymal tumors of the uterus.

BACKGROUND: Malignant mesenchymal uterine neoplasms are the most aggressive type of primary uterine tumors, with most patients dying within a few years of diagnosis. Thus, it would be very important to define prognostic factors for predicting the malignancy potential of at least some of their subtypes. METHODS: Flow cytometric cell cycle analysis (proliferative activity, DNA ploidy, and DNA index) was performed on archival paraffin embedded blocks from 80 patients with malignant mesenchymal uterine neoplasms (endometrial stromal sarcomas, malignant smooth muscle tumors, and malignant Müllerian mixed tumors). The Cox proportional hazards regression model was used to assess relative effects of the following factors on patient survival: clinical stage, mode of therapy, DNA+proliferative activity, DNA index, histologic type, cellularity, degree of atypia, mitotic activity, and depth of myometrial invasion. RESULTS: There were 9 low grade stromal sarcomas, 17 high grade stromal sarcomas, 8 smooth muscle neoplasms with uncertain malignant potential, 23 leiomyosarcomas, and 16 homologous and 7 heterologous malignant Müllerian mixed tumors. In univariate analysis for stromal sarcomas, statistical significance was found for DNA ploidy+proliferative activity (P < 0.001), histologic type (P = 0.005), and DNA index (P < 0.001). In multivariate analysis, DNA index appeared to be the only significant parameter influencing patient survival (P = 0.005). In univariate analysis for malignant smooth muscle neoplasms, statistical significance was detected for mitotic activity (P = 0.049) and International Federation of Gynecology and Obstetrics classification (P = 0.021), but in multivariate analysis, clinical stage appeared to be the only significant parameter influencing patient survival (P = 0.032). In univariate analysis for malignant Müllerian mixed tumors, statistical significance was found for the depth of myometrial invasion (P = 0.039), DNA index (P = 0.037), and clinical stage (P = 0.013), but in multivariate analysis, only the depth of myometrial invasion (P = 0.036) and clinical stage (P = 0.025) were of statistical significance. CONCLUSIONS: The most powerful prognostic indicator for stromal sarcomas was the DNA index, for malignant smooth muscle neoplasms it was the clinical stage, and for malignant Müllerian mixed tumors it was the depth of myometrial invasion.

Analysis of Variance↗
PubMed1994

Tumor cell cycle in patients with stage I endometrial carcinoma.

Flow cytometric cell cycle analysis was performed on paraffin-embedded blocks from 49 patients with stage I endometrial carcinoma. Care was taken to separate tumor tissue from normal tissue in each specimen; normal tissue was used as a control for each individual specimen. DNA index, proliferative activity, and cell DNA aneuploidy were correlated with known parameters of tumor malignancy. Increased DNA index corresponded well with the DNA aneuploid tumors, poor tumor differentiation (G3), myometrial invasion of more than one-third, more malignant histologic type of tumor, and low concentration of estrogen (< or = 10 fmole/mg) and progesterone (< or = 25 fmole/mg) receptors. Similar results were obtained for tumor cell proliferative activity (percentage of cells in S + G2/M phases) and for DNA aneuploid tumors. Since more than 90% of patients with stage I endometrial carcinoma survived the 5-year postoperation period, analyzed parameters could not be checked for survival-related prognostic significance. However, our data indicate that cell cycle analysis may be instrumental for objective ranking of several known prognostic parameters.

Adult↗
PubMed1994

Prognostic value of steroid hormone receptors concentration in patients with endometrial carcinoma.

The concentrations of progesterone receptors in endometrial tumors of 160 patients were analyzed with respect to survival and presence of clinicopathologic prognostic factors. The concentration of 25 fmol/mg of proteins for progesterone receptors appeared to be most powerful for prediction of survival. The survival was also significantly correlated to age, depth of myometrial invasion and clinical stage of the tumor. Concentration of estrogen receptors could not be correlated with the probability of patients' actuarial survival.

Actuarial Analysis↗
PubMed1990

Dynamic scintigraphy of the oesophagus in the evaluation of reflux oesophagitis.

A method is presented which combines gastro-oesophageal reflux quantification and oesophageal transit measurement so as to differentiate true reflux from residual oesophageal activity. A group of 33 subjects with gastro-oesophagitis reflux symptoms and endoscopically confirmed reflux oesophagitis and a group of 21 asymptomatic subjects with normal oesophageal, gastric and duodenal endoscopic findings were examined. The subjects were given 37 MBq 99mTc-Sn-colloid in saline orally and then scintiscanned dynamically. The gastro-oesophageal quantification was done after transit measurement and after the oesophageal time activity (to detect residual oesophageal activity) reached its minimum. The difference in the reflux indices between the two groups was highly significant. In low-grade oesophagitis measured reflux was lower than in higher grades of disease. Only 4.7% false-positive results were observed with a specificity of 95%, indicating that this method may be superior to methods published earlier.

Adult↗
PubMed1976

[Abdominal pregnancy].

A 38-year-old woman, after a spontaneous abortion following extrauterine pregnancy, developed abdominal pregnancy which was diagnosed only 6 days before the term of delivery, although she had been regularly examined. In the first trimester of that pregnancy she wanted to interrupt pregnancy and an alleged artificial abortus was performed. By cesarean section a live, at term baby, 3000/50, was born without any visible malformations. The placenta was left in situ because it partly adhered to the colon sigmoideum, mesosigma, and the wall of the small pelvis. Although it is better in abdominal pregnancy with cesaren section to remove the placenta immediately, the authors think that to leave the placenta in situ is safer if its position is such that to remove it would expose the patient to great risk.

Adult↗
PubMedDate not supplied

[The captopril test--an aid in the detection of scarring nephropathy as a cause of arterial hypertension in children].

Renal scarring with and without vesicoureteral reflux (VUR) has been now recognized as an important cause of paediatric hypertension for many years [1-5]. However, its pathogenesis has still remained uncleared. The widespread concept implicated the activation of renin-angiotensin system finding a powerfull support in higher peripheral plasma renin activity (PRA) in children with reflux nephropathy than in controls [6, 7] and in beneficial antihypertensive effects of ACE inhibitors. The latter, in form of captopril, has also been used in captopril test and in renal scintigraphy and isotope renography following the administration of captopril to provide evidence for renin dependent hypertension [8, 9]. Published studies of captopril test have centred on the identification of renovascular as opposed to essential hypertension [10-18, 20-22]. The aim of our study was to assess the usefulness of captopril test in differentiation between hypertensive children with renal scarring from those with essential hypertension. We studied blood pressure (BP) and PRA responses to a single dose of captopril in two groups of hypertensive children. Group A consisted of 29 patients, 14 boys and 15 girls, who had renal scaring as demonstrated by renal 99mTc dimercaptosuccinid acid scan (99m Tc DMSA) and/or intravenous pyelography. Group B included 19 patients, 19 boys and 10 girls who had arterial hypertension, while clinical examination excluded renal and other definable causes of BP elevation, and they were therefore considered to have essential hypertension. At the time of the study all patients had normal glomerular filtration rate and were not salt depleted. They did not receive any antihypertensive medication for at least two weeks. The test was performed in the morning in fasting sitting patients. At the start of the test a small vein in the hand or forearm was cannulated to permit blood sampling. BP was measured 10, 20, and 30 minutes before captopril administration to get baseline BP (mean of these three measurements) and to allow the children to become accustomed to the test procedure. A single oral dose of captopril 0.64 +/- 0.04 mg/kg body weight was given to patients from group A and almost the same dose of captopril, 0.63 +/- 0.05 mg/kg body weight, to patients from group B. The patients remained sitting and BP was measured every 15 minutes during an hour. Blood for PRA was drown in the sitting position (17 patients from group A and 16 patients from group B) before and one hour after the dose of captopril. Samples of blood for basal PRA were collected from 16 patients from group A and in 14 patients from in B in lying position after waking up in the morning. PRA was measured by radioimmunoassay using a commercially available kit, SB-REN 2, from CIS Bio International. According to the criteria of Muller et al. [10] the captopril test was positive if the post-captopril PRA (ng/ml/h) was greater than or equal to 12 with an increase of greater than or equal to 10 and relative increase of greater than or equal to 15% (400% if initial PRA was < 3). The results of our study are presented in Tables 1 and 2 and in Graphs 1 and 2. The age of patients, doses of captopril, initial BP and PRA before the use of captopril did not much differ between studied groups. Fall of BP and PRA increase were highly significant (p < 0.001) both in group A and group B. However, the hypotensive reaction of diastolic BP and MAP were more pronounced in group A (14.45 +/- 1.67% and 15.81 +/- 1.62%) than in group B (6.95 +/- 2.21% and 8.96 +/- 1.75%; p < 0.01), but there were no significant differences in PRA and systolic BP changes and positive results of captopril test between the studied groups. Hypotensive responses of diastolic BP and MAP greater than 10% of initial values were found to be more frequent in group A (79.32% and 79.31%) than in group B (26.61% and 31.57 degrees %; p < 0.001 and p < 0.01). Diastolic BP and MAP were directly related to the dose of cap

Adolescent↗
PubMedDate not supplied

[Histopathologic characteristics of bone marrow in patients with aplastic anemia].

INTRODUCTION: Histopathologic findings in bone marrow biopsies in patients are with aplastic anaemia crucial for the diagnosis of the disease. Changes observed by the examination of bone marrow such as cellularity, histopathologic type, presence of irregular cells maturation in all three blood lineages, condition of medullar stroma can be of great prognostic significance [1, 2, 4, 5]. The aim of the study was to make histopathological analyses in patients with aplastic anaemia and to establish parameters significant for the prognosis of the disease. PATIENTS AND METHODS: Thirty-three patients with aplastic anaemia treated in the Institute of Haematology, Clinical Centre of Serbia, from 1988 to 1995 were included in the study. Bone marrow specimens were analysed for: cellularity, number of megakaryocytes, condition of medullar stroma, presence/absence of blood and oedema, lymphoid elements, presence of plasma and reticular cells, and determination of histopathologic type on the basis of bone marrow changes. For data description we used the arithmetic mean and standard deviation for parametric results. Results of analyses were explained by Kaplan-Mayer's method and two factors analysis of variance. RESULTS: In all analysed marrow biopsy specimens a 100% hypocellularity was observed: 1. Weak hypocellularity in two specimens (6%); 2. Mild hypocellularity in six cases (19%); 3. Severe hypocellularity in 24 (75%), In one case (3%) the finding was not known. In 25 specimens (75%) megakaryocytes were not found, in 5 (16%) they were rare; in two cases (6%) they were numerous. Medullar stroma changes were not found in 30 (94%) byopsies, but in two (6%) they were found. Blood and oedema liquid were found in 26 (81%) cases and not in 6 (19%). Lympho-plasmocytic bone marrow infiltration was found in 23 specimens (72%) and not in nine (28%). First histopathologic types two (one-a and one-b) were present in 25 (78%) cases, histopathologic type two in 5 (16%) and type three in 2 (6%) specimens. The two factors analysis of variance regarding proportions between dead and alive patients revealed a significant difference in proportions according to histopathologic types (FD = 7.52; DF = 2; p = 0.002). Significant statistical differences were found between alive and dead patients in all three histopathologic types, tip one (one-a and one-b) (FD = 8; DF = 1; p = 0.06); type two (FD = 4.286; DF = 1; p = 0.041) and type three (FD = 4.762; DF = 1; p = 0.031). Histopathologic type two had the best prognosis regarding survival probability; p = 0.800; in histopathologic type two survival prognosis was worse p = 0.300, and type three it was the worst, p = 0.000. With regard to survival time, there was no statistical difference in patients with weak and mild bone marrow hypocellularity (p = 0.487). We also compared patients with severe bone marrow hypocellularity with patients with weak and mild hypocellularity. The difference in survival was statistically significant (p = 0.026), Consequently, survival was shorter in patients with severe bone marrow hypocellularity. DISCUSSION: Bone marrow biopsy and histopathologic examination are necessary for definitive diagnosis of aplastic anaemia [1-3]. Cellularity estimation in bone marrow based on biopsy and histopathologic examination is more reliable than that made by bone marrow aspiration [7]. Aplastic anaemia is a disease that can be associated with abnormal clones development. Most frequently paroxysmal nocturnal haemoglobinuria, acute leukaemias and myelodysplastic syndrome occur. Therefore, repeated bone marrow biopsies are necessary to follow-up the course of the disease [10, 11]. The presence of megakaryocytes in bone marrow specimens has favourable prognostic significance. Their appearance is a reliable sign of disease improvement [6, 7]. However, lymphocytosis, plasmocytosis and damaged marrow stroma are markers of bad prognosis [7]. (ABSTRACT TRUNCATED)

Analysis of Variance↗
PubMedDate not supplied

[20 years' experience in the treatment of children with terminal renal insufficiency in Yugoslavia].

The first specialized haemodialysis (HD) paediatric centre in former Yugoslavia was established at the University Children's Hospital in Belgrade in January 1980. A total of 194 children (F: 98, M: 96), aged less than 19 years (10.12 +/- 4.23), were treated for renal replacement therapy (RRT) over 20 years. Average annual incidence rate was 1.59 per million of child population (pmcp) aged less than 19 years for the period 1980-1990 (former Yugoslavia) and 2.85 pmcp aged less than 19 years for the period 1990-2000 (present Yugoslavia). Reflux nephropathy was the most frequent underlying disease and accounted for 37.06% of total cases, while other primary renal diseases were: glomerulonephritis (GN) 17.26%, cystic/hereditary familial nephropathy 12.69%, congenital disease 11.68%, interstitial nephritis 5.58%, non-recovered tubular necrosis 3.55%, secondary GN 1.52% and 10.66% remained with doubtful diagnosis. HD was the first RRT in 84.02%, peritoneal dialysis (PD) in 14.43% and pre-emptive transplantation in 1.55% of all patients. A total of 53 patients (27.3% of total terminal renal failure (TRF) patients) received 56 kidney transplants (58.93% live related, 37.50% cadaveric, 3.57% live-non related). Actual survival in RRT was 64.53% 5 in years; 51.68% in 10 and 48.23% in 15 years. Patient survival in HD was significantly better over the last ten-year period than in the first ten-year period (35.88% vs. 75.75%; p < 0.005) as well as the survival of transplanted patients in the same two periods (67.62% vs. 95.45%). Graft survival was 79.85% in 5 and 70.50% in 10 years. Cardiovascular complications were the most common cause of death of patients on RRT (56.10 posto) followed by infection (24.39). On December 31, 1999, 54 patients on RRT were alive less than 19 years: 75.92% in HD; 22.22% with functioning graft and 1.85% on automatic PD. This is the first national-wide long-term study of incidence and aetiology of paediatric TRF and outcome of paediatric RRT in Yugoslavia.

Adolescent↗
PubMedDate not supplied

[Clear cell carcinoma of the female genital tract].

From 1980 to 1989 clear-cell carcinoma was diagnosed in 66(4.2%) out of 1562 adenocarcinomas of the female genital tract: in the ovary, 2.9% in the endometrium, 9.8% in the uterine cervix; 4.5% in the vagina, and 1.9% in the Fallopian tube. The diagnosis was made at clinical stage I in 26.9% of cases located in the ovary, 62.5% in the endometrium, and 42.8% in the uterine cervix. At stages III and IV the highest percentage of cases was diagnosed in the ovary -42.3% and 26.9%, respectively. The material was divided into the histological subtypes, and the glandular papillary was found in 54.5%, the tubulocystic in 31.8% and the solid in 13.6% of cases. The tubulocystic subtype showed the best prognosis, with the 50% five-year survival rate, while the glandular papillary subtype had 38.1%, and the solid subtype a 14.3 five-year survival rate was 66.6% for the ovary, 85% for the endometrium and 75% for the uterine cervix. For all other stages together (II, III, IV), the five-year survival rate was 0% for the ovary, 20% for the endometrium and 0% for the uterine cervix.

Adenocarcinoma↗