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Biomedical subjects

D Bang

Publications and source records attributed to D Bang.

62 records · Page 4Linked to original sources
PubMed1987

A histologic study on the fate of intradermally implanted epidermal cells in guinea pigs: a new method for evaluation of skin allograft survival.

The fate of allogeneic (strains 13, 2, and JY-1) and autologous epidermal cell (EC) suspensions injected intradermally was investigated histologically in JY-1 strain guinea pigs. Epidermal cells were found to proliferate actively in the dermis and form EC nests with central keratinization. The significant reject reaction associated with necrosis of the epidermal structures was seen in due time in the animals implanted with allogeneic ECs. We attempted to assess the effect of cyclosporin A (CYA) on skin allograft survival by observing the fate of strain 13 ECs implanted intradermally into the CYA-treated JY-1. Successful prolongation of allograft survival with CYA was clearly demonstrated by this method. This is considered to be a useful experimental way for evaluation of skin allograft survival and to be suitable for routine use.

Animals↗
PubMed1987

Electron microscopic observation on dark endothelial cells in erythema nodosum-like lesions of Behçet's disease with ultrastructural X-ray spectroanalysis.

We used electron microscopy and electron microscopic X-ray spectroanalysis to examine the biopsies taken from erythema nodosum-like lesions of 20 patients with Behçet's disease. Electron-dense degenerated endothelial cells were found in the microcirculatory beds of the dermis and subcutaneous fat. However, electron microscopic X-ray spectroanalysis failed to demonstrate the localization of either chlorine or base metals in those degenerated endothelial cells. Moreover, neither in ultrastructurally normal endothelial cells, nor in infiltrating macrophages of erythema nodosum-like lesions the positive deposit of chlorine or base metals could not be detected. The results of this analysis suggest that environmental factors do not play an etiologic role in this disease. Significance of the degeneration of endothelial cells is not clear present.

Behcet Syndrome↗
PubMed1987

Ultrastructure of lymphocyte-mediated fat-cell lysis in erythema nodosum-like lesions of Behçet's syndrome.

In this study, biopsy specimens from 18 patients with Behçet's syndrome were examined by electron microscopy with particular attention to the appearance of the lymphocyte-macrophage infiltrate into the interlobules of subcutaneous fat in erythema nodosum-like lesions. Electron microscopic evaluation revealed vacuolization changes of fat cells with detachment of their cell membrane from the basal lamina that permitted lymphocytes and macrophages to enter into this developed space. We believe that the detachment of fat cells from the basal lamina precedes invasion by lymphocytes which, in turn, attracts macrophages into the space. This eventually leads to fat-cell lysis accompanied by activation of macrophages, which causes further inflammation, completing the picture of panniculitis in the erythema nodosum-like lesions in Behçet's syndrome.

Adipose Tissue↗
PubMedDate not supplied

Presence of circulating antibodies to a disease-specific antigen on cultured human dermal microvascular endothelial cells in patients with Behçet's disease.

Behçet's disease is a chronic, multisystem disorder characterized by a recurrent inflammatory reaction. Antiendothelial cell antibodies have been detected in the serum from patients with autoimmune diseases with presenting vasculitis and it is assumed that they can induce damage to the endothelial cells. In this study, we detected antiendothelial cell antibodies in the serum from patients with Behçet's disease using an enzyme-linked immunosorbent assay, electrophoresis and immunoblotting. The cytolysis of human dermal microvascular endothelial cells (HDMEC) was measured using a cytotoxicity assay. The serum from 37.4% of Behçet's disease patients showed IgM antibodies against unstimulated HDMEC while the serum from 18.4% of patients showed an increase in IgM antibody titer after IFN-gamma pretreatment. The frequency of vasculitis was higher in the IgM-positive Behçet's disease patients than in the IgM-negative patients. In Western blotting, IgM-positive Behçet's disease serum reacted with the 44 kDa HDMEC surface antigen, whereas IgM-positive systemic lupus erythematosus serum reacted with the 81 kDa HDMEC surface antigen. The reactivity to the 44 kDa protein band was also observed in cultured human umbilical vein endothelial cells but not in fibroblasts, A431 cells or SK-MEL-2 cells. Serum from Behçet's disease patients incubated with human complement or mononuclear cells produced no significant lysis of HDMEC, and cultured HDMEC were resistant to antibody-dependent cell-mediated cytotoxicity. The results suggest that antibodies against antigens on the surface of endothelial cells may play a role in inducing vasculitis in Behçet's disease, not through a direct toxic effect of an antiendothelial cell antibody but by an indirect effect involving the activation of endothelial cells to produce cytokines.

Antibodies↗
PubMedDate not supplied

Electronmicroscopic observation of the basement membrane zone in focal dermal hypoplasia.

A 3-month-old boy had reticulated achromic and atrophic skin lesions on the right buttock and thigh, as well as partial syndactyly of the third and fourth toes of the right foot. With hematoxylin and eosin stain, an epidermal defect, abnormally located fat cell lobules, and absence of the upper and midportions of the dermis were observed, conforming to a typical histopathologic picture of focal dermal hypoplasia. Electron microscopic examination of the atrophic site showed loose collagen bundles, collagen fibers with loss of regular bands, abnormal fibroblasts, and disruption of the basement membrane zone. These defects in the basement membrane zone strongly suggest that abnormal formation of type IV collagen is associated with focal dermal hypoplasia, and that this abnormal formation of collagen is correlated with the clinical sign of skin atrophy.

Atrophy↗
PubMedDate not supplied

Clear cell papulosis: case report and literature review.

Clear cell papulosis is a newly described skin disease characterized by multiple white papules. Histopathologically, diagnostic clear cells were seen among the basal cells of the epidermis. We report clear cell papulosis on the lumbar area and buttocks of a 1-year-old girl.

Diagnosis, Differential↗
PubMedDate not supplied

Behçet's disease-like symptoms induced by the Herpes simplex virus in ICR mice.

Herpes simplex virus (HSV) infection has been suggested to be one of the etiologic or triggering factors in Behçet's disease. To test this hypothesis, 258 ICR mice were inoculated with HSV type 1 (KOS strain). After the induced infection, 86 mice (33.3%) died, 77 (29. 8%) showed Behçet's disease-like symptoms, and 95 (36.8%) had a healthy, normal appearance or just a single symptom. The symptoms included skin ulcers on the earlobe, neck, abdomen, back or face (57. 1%); eye syndromes (39.0%); partial hair loss (33.8%); genital ulcers (19.5%); bullae (11.7%); arthritis (5.2%); gastrointestinal ulcer (5.2%); and tongue ulcers (3.9%). The induced Behçet's disease-like symptoms are similar to the clinical manifestations of ulcers, uveitis, and arthritis which have been significant in diagnosing Behçet's disease in patients.

Animals↗