Biomedical subjects

D Barettino

Publications and source records attributed to D Barettino.

22 records · Page 2Linked to original sources

PubMed1989

Identification of the spI products of Balbiani ring genes in Chironomus thummi.

The spI fraction of high molecular weight secretory proteins was analysed in Chironomus thummi. These proteins are encoded by giant Balbiani ring (BR) genes which develop specifically in salivary gland cells. Each component of the spI fraction was studied electrophoretically from early and middle 4th instar larvae and prepupae, as well from galactose-treated larvae where changes in the relative puffing pattern of BR1 and BR2 are known to occur. The spI fraction consists of at least two bands with electrophoretic mobilities slower than those of the spI components of Camptochironomus. The slow migrating component remains throughout the 4th larval instar, while the amount of the faster component changes, being abundant in early 4th instar and prepupae, but not present (or very weak) in middle 4th instar. The correlated shifts in BR puffing pattern during these developmental stages suggest that the slow and fast components are encoded by BR2 and BR1. The spI fraction is modified by galactose treatment, the fast component being induced in parallel with a decrease in the slow component. These changes are correlated with changes in the steady-state levels of RNA: an increase in BR1 RNA and a decrease in BR2 RNA, and of proteins. These proteins could correspond to the spIb and spIa fractions allocated to BR2 and BR1, respectively, in Camptochironomus. After galactose treatment a new faster band sometimes appears, that could correspond to the spIc fraction of Camptochironomus. A possible spId equivalent was also identified. In conclusion the main features of the spI family in C. thummi are similar to those of spI in Camptochironomus.

Animals↗

PubMed1988

Telomeric DNA sequences differentially activated by heat shock in two Chironomus subspecies.

The patterns of puffing, transcription and protein synthesis under heat shock were analysed in polytene nuclei of Chironomus thummi piger, in comparison with those obtained in the closely related subspecies C. th. thummi. Most chromosomal heat shock puffs, as well as heat shock induced polypeptides, in C. th. piger paralleled those previously reported for C. th. thummi. Nevertheless, we found a striking difference in behaviour in the induction of telomeric Balbiani rings by heat shock in the two subspecies. Although homologous sequences were present at all the telomeres in both subspecies, they were not always transcriptionally activated by heat shock. The most frequently puffed telomeres were that of chromosome III R in C. th. thummi and that of chromosome IV R in piger. Transcription of the same sequences from both telomeric Balbiani rings (T-BR-III and T-BR-IV) occurred under heat shock. The enigmatic behaviour of telomeres and the functional significance of T-BRs are discussed in relation to possible equivalents in other Diptera.

Animals↗

PubMed1988

Induction of the heat-shock response by carbon dioxide in Chironomus thummi.

The effects of a set of stress treatments on gene expression of Chironomus thummi salivary gland cells have been analyzed. Among the treatments assayed, only during recovery from carbon dioxide have we observed a response similar to that previously described after heat-shock treatment: induction of the heat-shock puffs and synthesis of the heat-shock polypeptides. In these conditions, puffing and transcription of telomeric regions were observed, which led to the appearance of the temperature-inducible telomeric Balbiani ring T-BR-III. Other treatments failed to induce the heat-shock response, despite promoting real stress conditions to C. thummi larvae or salivary gland cells.

Animals↗

PubMed1988

Correlation between the activity of a 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole-insensitive puff and the synthesis of major heat-shock polypeptide, hsp70, in Chironomus thummi.

The induction of puff III-A3b, a major heat-shock puff in Chironomus thummi salivary cells, was insensitive to the transcription inhibitor 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole (DRB), whereas no transcriptional activity could be detected at the other heat-shock puffs in the presence of this drug. In these conditions, a polypeptide with the same Mr and isoform pattern as those of the major heat-shock polypeptide, hsp70, was synthesized. These results suggest that hsp70 is encoded by locus III-A3b. In addition to DRB insensitivity, incorporation of [3H]UTP on puff III-A3b took place in an in vitro transcription assay under low-salt conditions (100 mM NaCl); no labelling could be detected at the other heat-shock puffs under these conditions. Although DRB has been reported as a specific inhibitor of RNA polymerase II-directed transcription, and although the low-salt conditions were not propitious for the activity of this enzyme, RNA polymerase II was detected on puff III-A3b and on the other heat-shock puffs by immunofluorescence with anti-RNA polymerase II antibodies.

Animals↗