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D Barraud

Publications and source records attributed to D Barraud.

6 recordsLinked to original sources

Restricted genetic defects underlie human complement C6 deficiency.

Complement C6 homozygous deficiency (C6D) has been rarely observed in Caucasians but was reported at higher prevalence among African-Americans. We report on the molecular basis of C6D in seven unrelated black individuals of North or Central Africa descent who live in France. These patients have presented Neisseria meningitidis infection (four cases), focal and segmental glomerulosclerosis with hyalinosis (one case), systemic lupus erythematosus (one case) or Still's disease (one case). All patients exhibited undetectable antigenic C6 by using a sensitive ELISA assay. An additional four cases of complete C6 deficiency with no associated disease have been characterized after family studies. Exons 6, 7 and 12 have been described recently as the location of molecular defects on the C6 gene in randomly chosen black Americans. Genomic DNA from the seven patients were subjected to direct polymerase chain reaction amplification of these three exons. Nucleotide sequencing analysis of the amplified DNA fragments revealed a homozygous single-base deletion (1936delG) in exon 12 in three cases and four compound heterozygous deletions for a single base in exon 7 (1195delC) or in exon 6 (878delA) associated with the same deletion in exon 12 (1936delG). Our observations further establish the restricted pattern of genetic defects associated with homozygous C6 complement deficiency in individuals of African descent.

Adolescent↗

[Nosocomial infectious: the realities of an endless fight].

Nosocomial infections (N.I.), contracted while patients are being cured, are the direct price of lightning progress in medicine in the course of the last fifty years. Absolute paradox of the end of century, the very place, where the most famous surgical and medical events are achieved, is also the same scene where a patient may contract infections that sometimes lead to a fatal outcome. With 10000 deaths per year and millions of additional hospitalization days, NI have become a real Public Health problem. They reach especially patients but also the staff, and they can be found everywhere a treatment is given (hospital, clinic, patient's home). Paradoxically, the first great myth of anti-NI struggle is the discovery of antibiotics, one of the hugest advancements for humanity. An excessive antibiotherapy prescription, added to a laxism in basic hygiene actions (individual or collective) increased for the last thirty years this phenomenon, which has now become so extensive that international and national authorities are managing this problem. The fight versus NI is an endless struggle because treatments will always exist. The rising emergence of antibiotics bacterial multiresistance has promoted this whole subject called Hospital Hygiene, a discipline which, little by little, regains a dominating place in our Health establishments. Bacteria are liable for 85% of NI, while virus and fungi are responsible for 15%. Omnipresent on a milliards scale, ubiquitous, the bacterial species will be thwarted only if the staff, who is taking care of patients, shows a constant vigilance. Immunosuppressive pathologies, age and nutritional conditions of the patient, new invasive diagnosis or assistance methods, antibiotics selection pressure, and at least a mismanagement of individual Hygiene and/or of the premises, are the main conditions leading to the outbreak of NI. The NI occurs basically within an early period of ten days after the patient's care. Catheter urinary infections, broncho-pneumonic infections with ventilation assistance, post-operative wounds and catheter bacteremia are the main nosocomial pathologies found in France, and in other european countries or in the United States. Staphylococci, Enterococci, Enterobacteria and Pseudomonas are the leaders of responsible bacteria but any kind of bacterium, can generate a NI, due to associated risk factors. Committees against nosocomial infections (Clin) have been organized in France in every public health establishment in 1988, then in every private health establishment in 1999. Since 1992 inter-aeras coordination committees and one national technical committee were instituted. In that way was the struggle officially set up. At last the Health National Agency of Accredidation and Valuation (ANAES), within the scope of an improvement policy concerning the security of and the quality of treatments in every health establishment, accepted about ten "standards" or "criterions" recommending supervision, education, written proceedings, informations diffusion modes, indicators definition in hospital hygiene. 85% of NI are in relation with direct or indirect hand transmission: that is the main point. So the first measure versus NI is just a fact: "HANDWASHING". It is the most effective method, the least expensive but unfortunately the least steady process. Beyond the struggle versus NI, all iatrogenic risks must be fought with a coherent control strategy. This "multivigilance" is a hard job for every health professional because it must be a daily, steady, rigorous and multidisciplinary task. The "Clin" is the privileged place for abilities round-up with an effective and permanent contribution between clinical structures, microbiology labs, pharmacy, administrative and technical sections. Every people belonging to a care unit must be equally involved in the rising of NI which activated a real mobilization of politicians and professionals who have become sensitive, on a socio-economic and an ethical basis. It is a collective and individual problem. Furthermore, the strict use of basic rules in hospital hygiene appears to be the KEYSTONE of the struggle.

Cross Infection↗

[Strategy against nosocomial infections in France].

Because of their extent and due to the complexity of their management, nosocomial infections (NI) have become a public health problem in view of ethical and economical considerations. A decree, issued on 05.06.1988, organizes the supervision and the prevention of NI throughout the country and requires the setting up of a committee against nosocomial infections (CLIN) in every medical establishment. In 1992 five inter-areas coordination committees (C-CLIN) and one national technical struggle committee (CTIN) were established in France. A governmental plan was instituted in 1994. This plan, enacted in the 04.19.1995 text, focuses on the importance of reducing by 30% the frequency of NI by the year 2000. The creation of a NI cell in the Health Ministry and of operational hygiene teams in the hospitals have since then strenghtened the anti-infection system. The CLIN became a compulsory institution in the 1998 for private establishments and a new decree is to be issued at the end of 1999 and to come into force on 12.31.2001 at the latest. Finally, the decree n degrees 98-535 (07.01.1998) sets up the French Agencies for Health Safety and an Institute of Sanitary Supervision. Both structures are in charge of the surveillance system in Health establishments. The CTIN and the C-CLIN, together with groups of experts and learned societies (Superior Council for Public Hygiene in France, French Society for Hospital Hygiene, Health National Agency of Accreditation and Valuation), will edit official guide of technical recommendations. This ambitions strategy, based on education, information, supervision and prevention methods, requires a multidisciplinary mobilization and needs the compulsory support of the extire hospital community.

Cross Infection↗

[Metabolism and kinetics of ampicillin elimination in cirrhosis. Therapeutical consequences. (author's transl)].

In cirrhotic patients, the authors studied the modification of the pharmacokinetics of ampicillin in the plasma and in the ascitic fluid, as well as its concentration in the urine. The influence of the jaundice, the ascites and diuretics were studied. In cirrhosis, dilution and elimination of the antibiotic are modified, as is shown by the increase in T 1/2 alpha and T 1/2 beta. These anomalies seem to be due essentially to modifications in the distribution volume; the degree of hepatocellular insufficiency does not appear to be of importance. The ascites acts as an independent compartment, into which the antibiotic's passage in slight. The practical consequences are the following: less frequent injections, increasing of the fractionated doses, in situ injections of ampicillin in cases of infection of the ascitic liquid.

Ampicillin↗

[Metabolism and kinetics of ampicillin elimination in cirrhosis. Therapeutical consequences (author's transl)].

In cirrhotic patients, the authors studied the modification of the pharmacokinetics of ampicillin in the plasma and in the ascitic fluid, as well as its concentration in the urine. The influence of the jaundice, the ascites and diuretics were studied. In cirrhosis, dilution and elimination of the antibiotic are modified, as is shown by the increase in T 1/2 alpha and T 1/2 beta. These anomalies seem to be due essentially to modifications in the distribution volume; the degree of hepatocellular insufficiency does not appear to be of importance. The ascites acts as an independent compartment, into which the antibiotic's passage is slight. The practical consequences are the following: less frequent injections, increasing of the fractionated doses, in situ injections of ampicillin in cases of infection of the ascitic liquid.

Ampicillin↗