PubMed Health⌕ Search

Biomedical subjects

D Barutzki

Publications and source records attributed to D Barutzki.

18 recordsLinked to original sources

Oocysts of Neospora caninum, Hammondia heydorni, Toxoplasma gondii and Hammondia hammondi in faeces collected from dogs in Germany.

Faecal samples of 24,089 dogs were examined coproscopically in two veterinary laboratories in Germany between March 2001 and October 2004. In 47 dogs, oocysts of 9-14 microm size were found. Their morphology was similar to those of Hammondia heydorni and Neospora caninum. Samples of 28 of these dogs were further examined by inoculation into gerbils: seven isolates induced a specific antibody response against antigens of N. caninum NC-1 tachyzoites. This response suggests that the isolates contained N. caninum. In addition to H. heydorni (12 times isolated), Toxoplasma gondii occysts (twice) and Hammondia hammondi oocysts (twice) were observed in dog faeces. The latter findings suggest that coprophagia with a subsequent intestinal passage by dogs plays a role in the dissemination of coccidian parasites for which cats are definitive hosts. Five of the seven N. caninum (NC-GER2, NC-GER3, NC-GER4, NC-GER5, NC-GER6) and the two T. gondii isolates (TG-dgGER1, TG-dgGER2) were successfully passaged into cell culture and are now available for detailed characterization. In contrast to oocysts of other parasites, N. caninum oocysts were predominantly found between January and April (Fisher exact; P=0.038). In the sera of dogs shedding N. caninum, no reactions against the immunodominant antigens with apparent molecular weights of 19, 29, 30, 33 and 37 kDa of N. caninum tachyzoites were observed 3-5 weeks after shedding. However, the animals recognized a 152-kDa N. caninum antigen. Compared with those identified as H. heydorni, T. gondii or H. hammondi, N. caninum oocyst isolates were significantly smaller in length with the 75th percentiles <or=10.7 microm when measured in concentrated sucrose solution and smaller length-width ratios with the 75th percentiles <or=1.06. It may thus be possible to develop criteria for a preliminary identification of N. caninum in dog faeces based on the oocyst morphology.

Animals↗

Endoparasites in dogs and cats in Germany 1999-2002.

Infections with endoparasites in dogs and cats have been determined by analysing the results of faecal examinations (Flotation, MIFC, sedimentation, Baermann, smear, ProSpecT Giardia Microplate Assay). Samples of 8438 dogs and 3167 cats from the years 1999 until 2002 have been included in the investigation. 2717 dogs (32.2%) and 771 cats (24.3%) have been infected with endoparasites. In the infected dogs the following parasites have been identified: Class Nematodea: Toxocara canis: 22.4%, Toxascaris leonina: 1.8%, Ancylostomatidae: 8.6%, Trichuris vulpis: 4.0%, Capillaria spp.: 2.3%, Crenosoma vulpis: 0.9%, Angiostrongylus vasorum: 0.3%; Class Cestodea: Taeniidae: 1.2%, Dipylidium caninum: 0.4%, Diplopylidium/Joyeuxiella: 0.1%, Mesocestoides: 0.2%, Diphyllobothrium latum: < 0.1%; Class Sporozoea: Sarcocystis spp.: 9.0%, Cystoisospora spp.: 22.3%, C. canis: 8.0%, C. ohioensis: 17.0%, Hammondia/Neospora: 1.7%; Class Zoomastigophorea: Giardia spp.: 51.6%. In the 771 infected cats the following prevalences of parasites have been found: Class Nematodea: Toxocara mystax: 26.2%, Ancylostoma tubaeforme: 0.3%, Capillaria spp.: 7.0%, Aelurostrongylus abstrusus: 2.7%; Class Cestodea: Taeniidae: 2.6%, Dipylidium caninum: 0.1%; Class Sporozoea: Sarcocystis spp.: 2.2%, Cystoisospora spp.: 21.9%, C. felis: 15.3%, C. rivolta: 7.9%, Toxoplasma/Hammondia: 4.5%; Class Zoomastigophorea: Giardia spp.: 51.6%.

Animals↗

[Migration and transposition of Oesophagostomum quadrispinulatum larvae from feces].

Investigations on the migration and translation of free-living stages of Oesophagostomum quadrispinulatum, using faeces containing eggs as starting material, revealed that mostly third stage larvae and very few second stage larvae migrated out of faeces, whereas first stage larvae remained in the faeces. The emigration rates depended on ambient relative humidity. Compared with the control, third stage larvae emigrated out of faeces at rates of 0.3%, 1.6% and 12.2% at 50%, 75% and 100% relative humidity, respectively. Offering helminth-free faeces, emigrated third stage larvae returned into faces at rates of 0.4-1.2%, 5.8-17.7%, 39.0-52.7%, and 45.2-60.7% after 1 h, 24 h, 5 days and 14 days, respectively. After a period of 1, 2, 3 or 4 weeks staying out of faeces, emigrated third stage larvae returned into faeces at rates of 23.3%, 8.8%, 22.1% and 6.0%, respectively. An examination of the horizontal translation revealed that most of the third stage larvae migrated distances up to 80 cm and a few even up to 150 cm returning into helminth-free faeces.

Animals↗

[The effect of optical radiation on the migration of third stage larvae of Oesophagostomum quadrispinulatum out of feces].

The influence of optical radiation on the emigration of third stage larvae out of faeces was investigated by exposing faeces containing infective larvae of Oesophagostomum quadrispinulatum to broad-band radiation of a sun-simulating wavelength spectrum corresponding to a sunny day, a cloudy day, dawn, and a full-moon night, as well as to monochromatic radiation of different wavelength spectrum at dawn and to complete darkness. It was demonstrated that third stage larvae of O. quadrispinulatum were able to differentiate between daylight and darkness responding to different irradiances with very high emigration rates at irradiances corresponding to dawn, and significantly lower emigration rates corresponding to full-moon light, and darkness as well as a cloudy and sunny day. Infective larvae reacted to monochromatic radiation of different wave-length spectrum at dawn and showed significantly higher emigration rates at the violet, green, yellow, and red light wavelength compared to darkness.

Animals↗

Ectopic enterobiasis: a case report and review.

Enterobius vermicularis ('pinworm') is rarely found outside the gastro-intestinal tract. We describe a case of extra-intestinal pinworm abscess associated with an inguinal hernia in an adult. A brief review of the literature is given and possible mechanisms of tissue invasion are discussed.

Abscess↗

[Helminth infections in wild boars kept in enclosures in southern Germany: severity of infections and fecal intensity].

To determine the intensity of helminth infection and egg output with regard to age and sex of wild boar, stomachs and intestines of 124, lungs of 62, and livers of 39 animals from 5 enclosures in Southern Germany were examined. The evaluation of the intensity of infection showed 579.4 worms per animal for Hyostrongylus rubidus, 510.4 for Globocephalus longemucronatus, 476.9 for Oesophagostomum dentatum, 476.9 for O. quadrispinulatum, 254.2 for Metastrongylus pudendotectus, 176.9 for M. salmi, 140 for Physocephalus sexalatus, 56.9 for M. apri, 45.4 for Trichuris suis, and 3.3 for Ascaris suum. 33.6 liver flukes per infected wild boar were also found. Additionally 2 metacestodes of Taenia hydatigena on the serous liver coat of an adult sow were found. The worm burden with respect to age of the animals showed no or only minimal age related differences, whereas a large number of O. dentatum and O. quadrispinulatum was found in adults compared to younger wild boars. The evaluation of the intensity of infection with regard to sex of the animals showed higher values only for H. rubidus, O. dentatum, O. quadrispinulatum and P. sexalatus in males compared to female wild boars. The egg output intensity in the samples taken from the rectum was in average 3300 EpG for A. suum, 450.4 EpG for Oesophagostomum, 445.9 EpG for Globocephalus, 220 EpG for Trichuris, 207.9 EpG for Metastrongylus, 190.8 EpG for Hyostrongylus and 67 EpG for Capillaris. The egg output with respect to age showed higher values in younger wild boars than in adults.

Age Factors↗

Eimeria infections of sheep in northwest Germany.

To investigate the incidence of ovine Eimeria spp. and seasonal dynamics in oocyst output, fecal samples of sheep from three different management systems were collected monthly over a 1-year period and examined for oocysts. A total of 10 species of Eimeria were observed. The most frequent species were E. bakuensis, E. ovinoidalis, E. weybridgensis/crandallis, E. parva and E. ahsata whereas E. faurei, E. granulosa, E. intricata and E. pallida were found less often. Lambs passed larger numbers of oocysts in their feces than either ewes or yearlings.

Animals↗

[Parasitic diseases in sheep and goats in Germany].

A review is presented on the species spectrum, biology and epidemiology of endo- and ectoparasites of sheep and goats in Germany. Current therapeutic, prophylactic and metaphylactic measures are given and discussed.

Animals↗

[Helminth infections in wild boars in enclosures in southern Germany: species spectrum and infection frequency].

To determine the helminth fauna of wild boars, stomachs and intestines of 124, lungs of 62, and livers of 39 animals from 5 enclosures in Southern Germany were examined. The evaluation of the species composition revealed at least 13 species: Metastrongylus apri, M. salmi, M. pudendotectus, Physocephalus sexalatus, Hyostrongylus rubidus, Globocephalus longemucronatus, Ascaris suum, Trichuris suis, Oesophagostomum dentatum, O. quadrispinulatum, Fasciola hepatica, C. tenuicollis and Capillaria. The most frequently isolated species was M. salmi with 91.9%, followed by G. longemucronatus with 91.1%, M. apri with 88.7%, M. pudendotectus with 87.1%, P. sexalatus with 51.6%, O. dentatum with 46%, T. suis with 30.6%, H. rubidus with 14.5%, A. suum with 12.1% and O. quadrispinulatum with 8.1%. Differences in the prevalence of infection, determined for animals up to 1 year old, 1-2 year-old and adults, were minimal for Metastrongylus spp. and G. longemucronatus. P. sexalatus, T. suis and A. suum, however, occurred more often in 1 year old, whereas H. rubidus, O. dentatum and O. quadrispinulatum were isolated more often from adults. A correlation of the infection rate with the sex of the animals was not found.

Animals↗

[Subcutaneous dirofilariasis: infection with Dirofilaria repens].

A female patient resident in Germany is described, who had developed dirofilariasis presenting as a hard subcutaneous nodule at the glabella. Dirofilaria repens was isolated after surgical removal of the skin lesion. She was treated with diethylcarbamazine (Hetrazan) for 4 weeks. Exposures related to infection with Dirofilaria repens are discussed.

Animals↗

[The use of fenbendazole for controlling helminths in small zoo ruminants].

By means of coproscopical examination the anthelmintic efficacy of 5.0 mg fenbendazole/kg body weight and 0.714 mg/kg body weight, administered for seven consecutive days, was determined in three series of trials on small ruminants in the zoological gardens of Munich. Both doses proved to be highly effective against trichostrongylids; the egg output was reduced to an average of 100% and 80-100%, respectively. Despite of a partly lower efficiency after the multiple administration of a low level doses (7 x 0.714 mg fenbendazole/kg body weight) this is a sufficient and economical treatment for the control of the infestation with endoparasites in the - in this respect - difficult housing systems of zoological gardens.

Animals↗

[Endoparasitic infestation of wild hedgehogs and hedgehogs in human care with a contribution to therapy].

In order to confirm the prevalence of endoparasites fecal samples from 127 hedgehogs living outdoors as well as from 85 in an animal home and from 542 hedgehogs hibernating in private homes were examined. 52.0%-72.3% of the animals from natural surroundings proved to be infested with the lung worm and 72.3%-74.0% with Capillaria species of the intestine, respectively. Capillaria aerophila were found in 15.1%-40.7%, whereas coccidia (1.4%-12.9%) were less frequent. In animal homes and private care hibernating hedgehogs excreted larvae of Crenosoma striatum (23.5% and 21.0%, respectively), eggs of Capillaria species of the intestine (47.1% and 37.1%), and eggs of Capillaria aerophila (7.1% and 19.4%), but oocysts of Isospora rastegaievae were found to be predominant (44.7% and 32.3%). Proglottides of Hymenolepis erinacei and eggs of Brachylaemus erinacei appeared only in the faeces of 3 and 2 hedgehogs, respectively. Helminths of the lung and gut were already found in May, therefore it must be concluded that these parasites are able to survive the winter in the host during the hibernation period. Even young hedgehogs (400-500 g) were infected with Crenosoma and/or Capillaria spp. of the intestine, however, compared with the adults the excretion of eggs and larvae was rather low. The antiparasitic agent Ivermectin (0.3 mg/100 g body-weight) was effective against Crenosoma striatum (efficacy: 95.9%) and Capillaria spp. (100%); therefore it can be recommended as a new, well tolerated anthelmintic against nematodes of the hedgehog.

Animals↗