Chemical modification of timothy grass pollen antigen B--I. Loss of antibody binding properties.
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Animals↗
Biomedical subjects
D Begley
Publications and source records attributed to D Begley.
Association of melanoma tumor antigen activity with beta2-microglobulin.
The majority of melanoma tumor antigen activity present in melanoma extracts derived from fresh tumor tissue binds to a Sepharose-anti-beta2-microglobulin adsorbent. Removal of HLA antigens from the extracts of melanoma tissue by using a KBr flotation technique did not reduce either the tumor antigen activity of the extracts or the binding of melanoma tumor antigen (MTA) activity to the Sepharose-anti-beta2-microglobulin adsorbent. The complete blocking of MTA activity by pretreating the anti-beta2-microglobulin adsorbent with beta2-microglobulin and the lack of detectable MTA binding to a Sepharose anti-normal human serum adsorbent demonstrated the specificity of the binding of MTA to the anti-beta2-microglobulin adsorbent.
Antigens, Neoplasm↗
Preparation of high titered rabbit anti-mouse IgE sera.
A method for the consistent production of rabbit anti-mouse IgE sera is described. Rabbits were immunized intravenously with IgE-anti-normal mouse serum mixtures over 8 to 12 weeks. Ten microliters of the anti-mouse IgE sera completely neutralized all the mouse IgE in the serum (50 microliter) possessing a passive cutaneous anaphylaxis titer of 20,000.
Animals↗
The chemical characterization of a major allergen of timothy pollen.
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Allergens↗
Preliminary characterization of a major allergen of timothy grass pollen.
Previous studies with timothy pollen extracts demonstrated that a low molecular weight dialyzable fraction, antigen D, possessed the allergenic determinant of the major allergen of timothy pollen (allergen B). Bio-gel P-2 gel-filtration of the dialysate fraction resulted in the isolation of a fragment, antigen D3 with a molecular weight near 1000. The relationship of antigen D3 to the antigenic and allergenic determinants of allergen B was evaluated by passive cutaneous anaphylaxis (PCA) in guinea pigs sensitized with rabbit antitimothy sera and by allergen-induced histamine release from monkey lung tissue passively sensitized with the serum of timothy-sensitive patients. These studies demonstrated that 10 units of antigen D3 gave 1) a 70% inhibition of PCA reactions in guinea pigs challenged with allergen B, and 2) a 73% inhibition of allergen-induced histamine release from monkey lung tissue sensitized with timothy reagin and challenged with the crude pollen extract (WST). Although chemical characterization studies of the antigen D3 fragment are still being carried out it appears that the major components fo this fragment are 1) a flavonoid pigment - quercitin, 2) a disaccharide moiety - cellobiose, and 3) the amino acid threonine linked together in an )O-glycosidic type linkage.
Allergens↗