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Biomedical subjects

D Beier

Publications and source records attributed to D Beier.

At least 55 records · Page 3Linked to original sources
PubMed1991

Nuclear tRNA(Tyr) genes are highly amplified at a single chromosomal site in the genome of Arabidopsis thaliana.

We have examined the organization of tRNA(Tyr) genes in three ecotypes of Arabidopsis thaliana, a plant with an extremely small genome of 7 x 10(7) bp. Three tRNA(Tyr) gene-containing EcoRI fragments of 1.5 kb and four fragments of 0.6, 1.7, 2.5 and 3.7 kb were cloned from A. thaliana cv. Columbia (Col-O) DNA and sequenced. All EcoRI fragments except those of 0.6 and 2.5 kb comprise an identical arrangement of two tRNA(Tyr) genes flanked by a tRNA(Ser) gene. The three tRNA genes have the same polarity and are separated by 250 and 370 bp, respectively. The tRNA(Tyr) genes encode the known cytoplasmic tRNA(G psi ATyr). Both genes contain a 12 bp long intervening sequence. Densitometric evaluation of the genomic blot reveals the presence of at least 20 copies, including a few multimers, of the 1.5 kb fragment in Col-O DNA, indicating a multiple amplification of this unit. Southern blots of EcoRI-digested DNA from the other two ecotypes, cv. Landsberg (La-O) and cv. Niederzenz (Nd-O) also show 1.5 kb units as the major hybridizing bands. Several lines of evidence support the idea of a strict tandem arrangement of this 1.5 kb unit: (i) Sequence analysis of the EcoRI inserts of 2.5 and 0.6 kb reveals the loss of an EcoRI site between 1.5 kb units and the introduction of a new EcoRI site in a 1.5 kb dimer. (ii) Complete digestion of Col-O DNA with restriction enzymes which cleave only once within the 1.5 kb unit also produces predominantly 1.5 kb fragments. (iii) Partial digestion with EcoRI shows that the 1.5 kb fragments indeed arise from the regular spacing of the restriction sites.(ABSTRACT TRUNCATED AT 250 WORDS)

Base Sequence↗
PubMed1990

[Use of a monoclonal antibody against envelope protein gp51 of bovine leukemia virus in a test for the diagnosis of enzootic bovine leukosis].

A highly specific monoclonal antibody (mAb) directed against envelope protein gp51 and effectively bonding the antigen (Ag) on account of its high affinity from an unpurified Ag preparation was chosen for use in a double-sandwich enzyme immuno-assay (EIA) for diagnosis of bovine leukaemia virus (BLV). The epitopes recognised in bovine sera by the gp51-specific antibodies were at the same time properly exposed. Some parameters of major importance to testing were optimised (Ab and Ag quantities, dilution of bovine sera for testing). Preliminary testing of the double-sandwich EIA on selected bovine sera and comparison with both the immunodiffusion test and anti-BLV EIA confirmed its good diagnostic specificity and sensitivity. Hence, this double-sandwich EIA, developed by means of an mAB against gp51, on account of the possibility to use as Ag culture supernatant of the FLC cell line, is a sensitive, low-cost alternative to the anti-BLV EIA Dessau MTP which had so far been used. The double-sandwich EIA is recommended for use in final sanitation for its high analytical and diagnostic sensitivity.

Animals↗
PubMed1976

[Grass tetany in cattle].

An optimal magnesium supply of dairy cows during pasture period is still a pressing problem in our farms and husbandry. Experiences are described concerning the application of different magnesium concentrated preparations in cases of grass tetany in 1970 to 1975 in the district of Potsdam. In 224 cows with magnesium concentrations values in the serum below 1.8 mg/100 ml a 25% MgSO4-solution, Parevert and Vers. A, a test solution of VEB Tierarzneimittelwerk Leipzig are used as a metaphylactic treatment. Losses by grass tetany could be reduced after application of the named preparations. Also in cases of low dosage a further, at least temporary drop of magnesium values in the serum could be prevented. Metaphylactic measures are recommended for andangered herds.

Animal Feed↗
PubMed1976

[Studies on serum Mg and Ca levels in cattle with grass tetany on hypocalcemia before and following infusion with high Mg and medium or high Ca concentrations].

Behaviour of Mg and Ca content in the blood serum of 28 cattle with grass tetany and hypocalcaemia was evaluated at short time intervals after infusion of 500 ml of a solution A (containing 12 g Mg-adipat and 5 g Ca-gluconate/100 ml aqua dest.) and a solution B (containing 12 g Mg-adipate and 12 g Ca-gluconate/100 ml) respectively. After treatment with the last-named solutions there was evident a considerable increase of Mg-values in the blood serum for a longer period (up to 6 h), also in the case of distinct hypomagnesaemia while Ca-values of the circulating blood declined more quickly. Efficacy of treatment was the better the earlier infusion was performed after occurence of clinical signs. Application of solution B for treatment of grass tetany and paresis with tetany like symptoms is recommended for several reasons. Necessity of assurance the Mg and Ca supply after infusion is emphasized.

Animals↗
PubMed1975

[Phosphoric acid ester preparations used in cattle, swine and sheep with special reference to cholinesterase activity. 3. Use of phosphoric acid ester preparations and their effect on acetylcholinesterase activity in swine].

The use of organophosphorus preparations in the treatment of ectoparasites and endoparasites of pigs is discussed. Treatment of 43 store pigs with preparations produced in the German Democratic Republic by washing and by the pour-on method resulted in considerable inactivation of acetylcholinesterase, which implies good systemic action. Overdosage increased this effect, but without producing signs of toxicity. The oral dosages for pigs cited in the literature were confirmed. Repeated treatment was recommended for the best results, as pigs rapidly break down the preparations. It is necessary to take into account adverse effects of oral administration on palatability of the ration and on weight gain. Addition of a preparation to a batch of food for a group of pigs is likely to be less successful than individual dosing, which is the best method for sows.

Acetylcholinesterase↗
PubMed1975

[Phosphoric acid ester preparations used in cattle, swine and sheep with special reference to cholinesterase activity. 4. Use of phosphoric acid esters and their effect on acetylcholinesterase activity in sheep].

The use of organophosphorus preparations for the control of ectoparasites and endoparasites of sheep, particularly systemic application, is discussed. Experiments on 13 groups of sheep with five preparations produced in the German Democratic Republic in various formulations and concentrations showed that external application had good contact activity, but little was absorbed. Acetylcholinesterase activity was not inhibited, except by pour-on application of doses several times the normal dose. The preparations were arranged in order of cholinesterase inhibition. In contrast to cattle, diminished cholinesterase activity was unreliable as in indicator of systemic toxicity of organophosphorus preparations in sheep.

Acetylcholinesterase↗
PubMed1975

[Use of the latex test in the serodiagnosis of enzootic leukosis of cattle].

Two antigenic extracts, prepared by different methods, were tried on 317 serum samples in the microscope-slide agglutination, sedimentation and tube agglutination tests. In comparison with the haematological findings, both antigens and all the tests gave a proportion of non-specific positive results, which made it impossible to obtain specific results. Various ways of reducing the proportion of non-specific reactions were tried, without success.

Animals↗
PubMed1975

[Comparative studies with various anticoagulant stabilizer combinations for the preservation of blood specimen in leukosis diagnosis. 1. Studies on storage life of dry-preserved blood vials for the hematologic diagnosis of leukosis].

Four anticoagulants in current use were compared in blood samples used to prepare dried specimens. The most suitable was a mixture of one part of heparin solution (5000 IU strength) and four parts of distilled water. Blood samples treated in this way must be process at the laboratory within 16 hours. Specimens dried in tubes could be stored for a long time, and this was the best way of dealing with samples from individual or export cattle.

Animals↗
PubMed1975

[Comparative studies with various anticoagulant stabilizer combinations for the preservation of blood specimen in leukosis diagnosis. II. Testing the application possibilities of new anticoagulant stabilizer combinations for the hematologic diagnonsis of leukosis].

The anticoagulant Chelaplex III in aqueous solution with two new stabilizers (methenamine and tannin) was tested for the preservation of blood samples, together with another combination composed of oxalic acid and methenamine solution. The eventual aim was to test their suitability for use with blood specimens dried in the tube, but this was not done in the present experiments. It was shown that a combination of 5% oxalic acid with 36% methenamine was at least as good as the conventional Chelaplex III-formaldehyde combination for inhibiting coagulation, and it was relatively cheap.

Animals↗