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D Besluau

Publications and source records attributed to D Besluau.

9 recordsLinked to original sources
PubMed1988

Immune response to H-2 class I antigens on platelets. I. Immunogenicity of platelet class I antigens.

Purified allogeneic murine platelet suspensions were found unable to induce primary anti-H-2 class I antibody or T cell proliferative responses. In contrast, the same platelet suspensions could elicit secondary anti-class I responses. The secondary responses were not due to contaminating leucocytes. Possible explanations, the lack of acolyte determinants (class II or non- H-2) on platelets or inappropriate layout and/or structure of their class I antigens, are discussed. These findings emphasize the importance of sufficient leucocyte depletion before platelet transfusion in the human.

Animals↗
PubMed1988

Immune response to H-2 class I antigens on platelets. II. Specific decrease of H-2 class I-specific antibody response induced by treatment with allogeneic platelets.

Mice pretreated with injections of allogeneic platelets were found to mount a decreased antibody response upon challenge by lymphocytes of the same donor strain. This decrease was mediated by platelets themselves, and not by leucocytes and red cells contaminating the platelet suspension. It affected specifically antibodies reactive with H-2 class I antigens present on donor platelets. This phenomenon may be related to the lack of class II or some non-H-2 antigens on platelets, and/or to properties of their class I antigens (soluble molecules adsorbed from the plasma). These findings emphasize the potential usefulness of purified platelet transfusions preceding organ transplantation in man.

Animals↗
PubMed1979

The protective effect of some phenothiazine derivatives against streptolysin O.

1 Chlorpromazine (Cpz) and promethazine (Pmz) protected mice against lethal doses of streptolysin O (SLO) given intravenously. Three other phenothiazine derivatives had similar but lesser effects. 2 The protective effect developed slowly; maximal protection was obtained 4 h after subcutaneous injection of Cpz and Pmz. 3 The haemolytic activity of SLO in vitro was also inhibited by Cpz.

Animals↗
PubMed1977

[Effect of Corynebacterium parvum on the immune response in guinea pigs. I. Mode of enhancement of the anamnestic response and development of delayted hypersensitivity after treatment with Corynebacterium parvum].

The effect of Corynebacterium parvum (C. parvuum) on the immune response of the guinea pig to ovalbumin varies with the protocol of immunization. The marked effect of C. parvum on the anamnestic response in the rabbit has been confirmed in the guinea pig when immunization is carried out intradermally with a mixture of C. parvum and ovalbumin. When C. parvum is given intravenously or subcutaneously or intradermally but separately from the antigen, this effect is not observed. Whatever the route of injection guinea pigs treated with C. parvum show skin reactions of delayed type hypersensitivity at the site of an intradermal booster when the latter is given at least 27 days after primary immunization.

Animals↗
PubMed1977

[Effect of Corynebacterium parvum on the immune response in guinea pigs. II. Passive transfer of enhanced anamnestic response and delayed hypersensitivity observed after treatment with Corynebacterium parvum].

After intradermal immunization with a mixture of Corynebacterium parvum (C. parvum) and ovalbumin guinea pigs show a markedly increased anamnestic response to an intradermal booster of ovalbumin as compared to controls treated with ovalbumin only. At the same time a reaction of delayed type hypersensitivity is observed in the treated animals, but not in controls. The enhanced anamnestic response as well as the posivitive skin reaction were transferred to strain 2 histocompatible guinea pigs by peripheral blood leukocytes as well as by peritoneal exudate cells. Passive transfer was not obtained after prior irradiation of donor animals.

Animals↗
PubMedDate not supplied

H-2 influence on the production of real but not of apparent H-2-specific antibodies induced by the association of syngeneic class I heavy chains with bovine beta 2-microglobulin.

Immunogenic properties of class I molecules resulting from the association of mouse class I heavy chains with a xenogeneic beta 2-microglobulin (beta 2-m) were investigated by studying the antibody response of mice of injections to syngeneic Con A lymphoblasts, induced in conditions allowing the replacement of endogenous beta 2-m by exogenously added bovine beta 2-m provided by fetal calf serum (FCS-Con A blasts). Lymphocytotoxic antibodies were regularly produced and according to their specificities they could be divided into two types: antibodies whose reactivity was (1) dependent on and (2) independent of the presence of bovine beta 2-m on target cells. Although both types displayed an H-2 dependent polymorphic reaction pattern, only antibodies recognizing class I molecules without bovine beta 2-m can be considered as real H-2-specific antibodies. The others are only apparent H-2-specific antibodies: their polymorphic reaction pattern is dependent both on the presence of bovine beta 2-m on the surface of target cells and on their H-2 haplotype. A comparison of the antibody response of mice with various H-2 haplotypes to injections of syngeneic FCS-Con A blasts showed no significant difference in the induction of bovine beta 2-m-dependent antibodies (apparent H-2-specific) among the mice from all strains tested (H-2b, H-2p, H-2q, and H-2s). Unexpectedly, for most strains more than 60% of the immunized mice produced also beta 2-m-independent antibodies (real H-2-specific), with the exception of H-2q mice, in which only 30% of sera were positive. The real H-2-specific antibody response is of two types: some mice (H-2p and H-2s) produced antibodies only reactive with allogeneic target cells whereas others (H-2b and H-2q) produced in addition antibodies that were reactive with syngeneic cells. Thus H-2 appears to play an important role in the induction and specificity of the lymphocytotoxic H-2-specific antibodies induced upon immunization with cells expressing syngeneic class I heavy chains associated with bovine beta 2-m.

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