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Biomedical subjects

D Biou

Publications and source records attributed to D Biou.

5 recordsLinked to original sources
PubMed1992

Evaluation of the hepatotoxicological effects of a drug in an in vivo/in vitro model.

Both in vivo and in vitro models have certain disadvantages for the study of the chronic hepatotoxicity of drugs. The aim of this work was to evaluate a new approach based on an in vivo/in vitro model. After chronic in vivo treatment of rats with Vincamine and Vindeburnol (an eburnamenine derivative which exhibits hepatotoxic properties in man) liver cells were isolated, and functional and metabolic disorders (metabolic utilization of fructose and protein biosynthesis) were studied to determine injury. The results showed no modification of blood parameters, but a direct relationship between the dose of Vindeburnol administered in vivo and the metabolic disorders observed in vitro, evidencing the high sensitivity and reliability of this model.

Animals↗
PubMed1992

Analysis of carbohydrate-mediated heterogeneity and characterization of N-linked oligosaccharides of glycoproteins by high performance capillary electrophoresis.

Capillary zone electrophoresis (CZE) has been investigated as an alternative method to analyze the carbohydrate moieties of glycoproteins. Carbohydrate-mediated microheterogeneity of the recombinant plasminogen activator (rt-PA) was examined. The glycoprotein was resolved in multiple electrophoretic species using CZE but the separation was complicated by adsorption of the molecules to the wall of the capillary. The influence of several parameters, such as pH, molarity of the buffer and addition of a cationic additive, on the separation of glycopeptides was investigated. High resolution and reproducible separations of rt-PA glycopeptides carrying hybrid and complex type chains were obtained using either a 100 mM phosphate buffer, pH 6.6, or a 100 mM Tricine buffer, pH 8.2, containing 1.25 mM of putrescine. N-Oligosaccharides from fetuin, t-PA and alpha 1-acid glycoprotein were separated within 20 min on the basis of both their sialic acid content and their structure. The use of an oligosaccharide fingerprinting technique, such as the present one, could have many applications in biotechnology to assess, for example, the consistency of production of a glycoprotein or for analytical glycoprotein chemistry.

Animals↗
PubMed1979

[Isolation and identification of psychotropic drugs by circular chromatography (author's transl)].

The "SelectaSol Solvent System" manufactured by SCHLEICHER and SCHULL is convenient for practical circular chromatography. These new possibilities are suitable to the rapid determination of psychotropic drugs in biological fluids of patients in emergency clinical toxicology. We are comparing circular and linear techniques with regard to the choice of solvent systems, diffusion time, isolation and characterization of implicated drugs.

Antidepressive Agents, Tricyclic↗
PubMed1977

[Laurell electroimmunodiffusion. Concerns and limits of various technics for the study of immunoaffinity chromatographic eluants].

Monodimensional and bidimensional electroimmunodifusion techniques were applied to the study of immunochromatography eluates. They were obtained with human sera as samples, and rabbit anti human sera alpha1AGP antibodies coupled to CNBr Sepharose 4B packed in a chromatography column. By monodimensional electroimmunodiffusion alpha1AGP could be quantitated. By tandem bidimensional immunoelectrophoresis the eluate purity could be shown, as well as its identity to alpha1AGP. Besides, this last technique was used to the critical study of elution conditions, and to put forward the influence of some parameters (such as pH ionic strength) on alpha1AGP denaturation and its transformation into a protein which is still antigenic but has lost a part of its electrophoretic mobility at pH 8.6. This phenomenon could explain the alpha1AGP loss observed after column elution. The precautions in the interpretation, the interest and the sensitiveness of the electroimmunodiffusion techniques are exposed.

Antibodies↗