[Aging and oxidative stress. Which potential markers?].
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Aged↗
Biomedical subjects
D Bonnefont-Rousselot
Publications and source records attributed to D Bonnefont-Rousselot.
[Study of oxidative stress in the elderly].
OBJECTIVES: Determine the oxidative stress status and its significance in elderly subjects. METHODS: Six parameters marking oxidative stress evaluated in 52 elderly patients (mean age 85 +/- 6 years; range 74 to 98) admitted to a medium-term and long-term nursing home (n = 30) or a hospital ward (n = 22) were compared with those in 30 disease-free young subjects (age range 20-40 years). Plasma levels of thiobarbituric acid reactive substances vitamin E and selenium and activity of free-radical protective enzymes (erythrocyte superoxide dismutase, plasma and erythrocyte glutathione peroxidase) were assessed. RESULTS: Thiobarbituric acid reactive substances were higher and superoxide dismutase, erythrocyte and plasma glutathione peroxidase, and plasma selenium were lower in elderly patients than in young controls. There was no difference in vitamin E levels. In the nursing home population, erythrocyte superoxide dismutase was correlated with erythrocyte glutathione peroxidase and vitamin E, plasma glutathione peroxidase with erythrocyte glutathione peroxidase, vitamin E and selenium and erythrocyte g peroxidase, vitamin E and selenium and erythrocyte glutathione peroxidase with vitamin E. Only the correlation between erythrocyte and plasma glutathione peroxidase was found in the hospitalized population. These levels remained unchanged for a 30 day period in individual patients. CONCLUSION: "Oxidative stress" assessed by six parameters was thus observed in the elderly population and could be considered as a "biological marker of ageing". Supplementation with selenium or other anti-oxidants could be proposed.
Adult↗
[Evaluation of lipid peroxidation by measuring thiobarbituric acid reactive substances].
Malondialdehyde assay is the most generally used test in the appreciation of the role of oxidative stress in disease. Malondialdehyde is one of several products formed during the radical induced decomposition of polyunsaturated fatty acids. Most often, malondialdehyde assay used its reactivity at high temperature and low pH, towards thiobarbituric acid. This reaction is very sensitive but its specificity, even with improvement of pre-analytical (sampling, preservatives), and analytical stages (fluorescence, HPLC) is still a matter of debate. At present, the concept of "thiobarbituric acid reactive substances" (TBARS) have merged and progressively replaced the initial malondialdehyde assay. In this review, we presented the main results concerning the assays of TBARS and malondialdehyde in blood and different biological medium. In the future, oxidative stress appreciation will need the precise analytical determination of different molecules triggered by free radicals. The TBARS assay should be considered as a global test, allowing a global approach of lipoperoxidation whereas specific determination of malondialdehyde can only appreciate one of the end-product formed during oxidative stress.
Animals↗